RT Journal Article SR Electronic(1) A1 Okahashi, Nobuo A1 Sasakawa, Chihiro A1 Okada, Nobuhiko A1 Yamada, Masatoshi A1 Yoshikawa, Masanosuke A1 Tokuda, Masayuki A1 Takahashi, Ichiro A1 Koga, ToshihikoYR 1990 T1 Construction of a NotI restriction map of the Streptococcus mutans genome JF Microbiology, VO 136 IS 11 SP 2217 OP 2223 DO https://doi.org/10.1099/00221287-136-11-2217 PB Microbiology Society, SN 1465-2080, AB Streptococcus mutans and Streptococcus sobrinus are the major causative organisms of human dental caries. Pulsed-field gel electrophoresis (PFG) showed that the restriction enzyme NotI produced ten and six DNA fragments from the genomes of S. mutans strain MT8148 and S. sobrinus strain 6715, respectively. The sizes of the chromosomes of S. mutans and S. sobrinus were each estimated to be about 2200 kb. The NotI restriction map of S. mutans MT8148 genome was constructed by Southern blot analysis with probes that overlapped two adjacent NotI fragments. Several virulence-associated genes of S. mutans were placed on the NotI restriction map. In addition, unique ‘fingerprints’ of S. mutans chromosomal DNA digested with NotI were produced by PFG, and these may be useful for epidemiological studies., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-136-11-2217