1887

Abstract

The decay rate of the potential to synthesize proteins after inhibition of transcription with rifampicin (Rif) was analysed at different times of energy and nutrient starvation for the marine sp. S14. The decline of protein synthesis following Rif treatment is due to the instability of mRNA and permits an estimate of the functional mRNA half-life. The half-life of the mRNA pool was found to increase 6-fold (from 1·7 to 10·3 min) during a period of 24 h of total energy and nutrient starvation. To resolve whether the increase in the mean mRNA half-life was a result of the stabilization of the entire pool or if proteins expressed during starvation were translated from very stable mRNAs, the half-lives of specific mRNAs were measured. It was found that the half-lives of mRNAs common to both growing and starving cells were increased between 2- and 3-fold during a period of 24 h starvation, and that some starvation-specific proteins were encoded by extremely long-lived mRNAs (up to 70 min). The possible role of stabilization of mRNA as a mechanism to economize protein synthesis during starvation conditions is discussed

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-11-2195
1990-11-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/11/mic-136-11-2195.html?itemId=/content/journal/micro/10.1099/00221287-136-11-2195&mimeType=html&fmt=ahah

References

  1. Albertson N. H., Jones G. W., Kjelleberg S. 1987; The detection of starvation-specific antigens in two marine bacteria. Journal of General Microbiology 133:2225–2231
    [Google Scholar]
  2. Albertson N. H., Nyström T., Kjelleberg S. 1990a; Exoprotease activity of two marine bacteria during starvation. Applied and Environmental Microbiology 56:218–223
    [Google Scholar]
  3. Albertson N. H., Nyström T., Kjelleberg S. 1990b; Macromolecular synthesis during recovery of the marine Vibrio sp. S14 from starvation. Journal of General Microbiology 136:2195–2199
    [Google Scholar]
  4. Belasco J. G., Chen C. -Y. A. 1988; Mechanism of puf mRNA degradation: the role of an intercistronic stem-loop structure. Gene 72:109–117
    [Google Scholar]
  5. Belasco J. G., Higgins C. F. 1988; Mechanisms of mRNA decay in bacteria: a perspective. Gene 72:15–23
    [Google Scholar]
  6. Belasco J. G., Nilsson G., Von Gabain A., Cohen S. N. 1986; The stability of E. coli gene transcripts is dependent on determinants localized to specific mRNA segments. Cell 46:245–251
    [Google Scholar]
  7. Collins J. J., Roberts G. P., Brill W. J. 1986; Posttranscriptional control of Klebsiella pneumoniae nif mRNA stability of the nifL product. Journal of Bacteriology 168:173–178
    [Google Scholar]
  8. Erickson J. W., Vaughn V., Walter W. A., Neidhardt F. C., Gross C. A. 1987; Regulation of the promoters and transcripts of rpoH, the Escherichia coli heat shock regulatory gene. Genes and Development 1:419–432
    [Google Scholar]
  9. Von Gabain A., Belasco J. G., Schottel J. L., Chang A. C. Y., Cohen S. N. 1983; Decay of mRNA in Escherichia coli: investigation of the fate of specific segments of transcripts. Proceedings of the National Academy of Sciences of the United States of America 80:653–657
    [Google Scholar]
  10. Gorski K., Roch J. -M., Prentki P., Krisch H. M. 1985; The stability of bacteriophage T4 gene 32 mRNA: a 5′ leader sequence that can stabilize mRNA transcripts. Cell 43:461–469
    [Google Scholar]
  11. Ingraham J. K., Maaløe O., Neidhardt F. C. 1983 Growth of the Bacterial Cell Sunderland, USA: Sinauer Associates Inc;
    [Google Scholar]
  12. Kennell D., Simmons C. 1972; Synthesis and decay of messenger ribonucleic acid from the lactose operon of Escherichia coli during amino-acid starvation. Journal of Molecular Biology 70:451–462
    [Google Scholar]
  13. Kurath G., Morita R. Y. 1983; Starvation-survival physiological studies of a marine Pseudomonas sp. Applied and Environmental Microbiology 45:1206–1211
    [Google Scholar]
  14. Mårdén P., Nyström T., Kjelleberg S. 1987; Uptake of leucine by a Gram-negative heterotrophic bacterium during exposure to starvation conditions. FEMS Microbiology Ecology 45:233–241
    [Google Scholar]
  15. Melefors Ö., Von Gabain A. 1988; Site-specific endonucleolytic cleavages and the regulation of stability of E. coli ompA mRNA. Cell 52:893–901
    [Google Scholar]
  16. Morita R. Y. 1985; Starvation and miniaturisation of heterotrophs, with special emphasis on maintenance of the starved viable state. In Bacteria in Their Natural Environments pp. 111–130 Fletcher M. M., Floodgate G. D. Edited by London: Academic Press;
    [Google Scholar]
  17. Morita R. Y. 1988; Bioavailability of energy and its relationship to growth and starvation survival in nature. Canadian Journal of Microbiology 34:436–441
    [Google Scholar]
  18. Nelson D. R., Zusman D. R. 1983; Evidence for long-lived mRNA during fruiting body formation in Myxococcus xanthus . Proceedings of the National Academy of Sciences of the United States of America 80:1467–1471
    [Google Scholar]
  19. Nilsson G., Belasco J. G., Cohen S. N., Von Gabain A. 1984; Growth-rate dependent regulation of mRNA stability in Escherichia coli . Nature; London: 31275–77
    [Google Scholar]
  20. Nyström T., Kjelleberg S. 1989; Role of protein synthesis in the cell division and starvation induced resistance to autolysis of a marine Vibrio during the initial phase of starvation. Journal of General Microbiology 135:1599–1606
    [Google Scholar]
  21. Nyström T., Mårdén P., Kjelleberg S. 1986; Relative changes in incorporation of leucine and methionine during starvation survival of two bacteria isolated from marine waters. FEMS Microbiology Ecology 38:285–292
    [Google Scholar]
  22. Nyström T., Albertson N., Kjelleberg S. 1988; Synthesis of membrane and periplasmic proteins during starvation of a marine Vibrio sp. Journal of General Microbiology 134:1645–1651
    [Google Scholar]
  23. Nyström T., Albertson N., Kjelleberg S. 1989; Physiological and molecular adaptation to non-growth by marine vibrios. In Recent Advances in Microbial Ecology pp. 80–84 Hattori T. Others Edited by Tokyo: Japan Scientific Societies Press;
    [Google Scholar]
  24. Pato M. L., Von Meyenburg K. 1970; Residual RNA synthesis in Escherichia coli after inhibition of initiation of transcription by rifampicin. Cold Spring Harbor Symposia on Quantitative Biology 35:497–504
    [Google Scholar]
  25. Pato M. L., Bennett P. M., Von Meyenburg K. 1973; Messenger ribonucleic acid synthesis and degradation in Escherichia coli during inhibition of translation. Journal of Bacteriology 116:710–718
    [Google Scholar]
  26. Pedersen S., Reeh S., Friesen J. D. 1978; Functional mRNA half lives in E. coli . Molecular and General Genetics 166:329–336
    [Google Scholar]
  27. Petersen C. 1987; The functional stability of the lacZ transcript is sensitive towards sequence alterations immediately downstream of the ribosome binding site. Molecular and General Genetics 209:179–187
    [Google Scholar]
  28. Reeve C. A., Amy P. S., Matin A. 1984; Role of protein synthesis in the survival of carbon-starved Escherichia coli K-12. Journal of Bacteriology 160:1041–1046
    [Google Scholar]
  29. Schneider E., Blundell M., Kennell D. 1978; Translation and mRNA decay. Molecular and General Genetics 160:121–129
    [Google Scholar]
  30. Stanssens P., Remaut E., Fiers W. 1986; Inefficient translation initiation causes premature transcription termination in the lacZ gene. Cell 44:711–718
    [Google Scholar]
  31. Zusman D. R., Cumsky M. G., Nelson D. R., Romeo J. M. 1986; Myxobacterial hemagglutinin: a developmentally induced lectin from Myxococcus xanthus . In Microbial Lectins and Agglutinins pp. 197–215 Mirelman D. Edited by New York: John Wiley;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-11-2195
Loading
/content/journal/micro/10.1099/00221287-136-11-2195
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error