SUMMARY: The decay rate of the potential to synthesize proteins after inhibition of transcription with rifampicin (Rif) was analysed at different times of energy and nutrient starvation for the marine sp. S14. The decline of protein synthesis following Rif treatment is due to the instability of mRNA and permits an estimate of the functional mRNA half-life. The half-life of the mRNA pool was found to increase 6-fold (from 1 ·7 to 10·3 min) during a period of 24 h of total energy and nutrient starvation. To resolve whether the increase in the mean mRNA half-life was a result of the stabilization of the entire pool or if proteins expressed during starvation were translated from very stable mRNAs, the half-lives of specific mRNAs were measured. It was found that the half-lives of mRNAs common to both growing and starving cells were increased between 2- and 3-fold during a period of 24 h starvation, and that some starvation-specific proteins were encoded by extremely long-lived mRNAs (up to 70 min). The possible role of stabilization of mRNA as a mechanism to economize protein synthesis during starvation conditions is discussed


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error