1887

Abstract

Cyanophycin, the nitrogen reserve compound in cyanobacteria, has a dynamic metabolism during transitions between the metabolic states of nitrogen deficiency and nitrogen repletion and vice versa. Cyanophycin was transiently synthesized when ammonia-grown or 6308 consumed a limited amount of ammonia. It was synthesized during the phase of declining ammonia concentration, and its degradation was complete by the time the external ammonia had been completely consumed. When nitrogen-starved cells of or 6308 were given a usable source of fixed nitrogen, cyanophycin again accumulated transiently. This synthesis of cyanophycin was triggered by the suddenly renewed availability of fixed nitrogen, and not by the subsequent decline in the external concentration of fixed nitrogen. The cyanophycin was degraded when balanced exponential growth was again possible. Transient accumulations of cyanophycin with similar kinetics were also observed when ammonia was added to nitrogen-fixing It is suggested that cyanophycin serves as a dynamic reservoir which separates the environmental supply of fixed nitrogen from the metabolic demands of the cells, and that this provides a mechanism which enables cyanobacteria to maximize their share of any available fixed nitrogen. This would give cyanobacteria a competitive advantage over other organisms.

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1990-10-01
2024-12-05
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References

  1. Allen M-B., Arnon D.I. 1955; Studies on nitrogen-fixing blue- green algae. I. Growth and nitrogen fixation by Anabaena cylindrica Lemm. Plant Physiology 30:366–372
    [Google Scholar]
  2. Allen M.M. 1968; Simple conditions for the growth of unicellular blue-green algae on plates. Journal of Phycology 4:1–4
    [Google Scholar]
  3. Allen M.M. 1984; Cyanobacterial cell inclusions. Annual Review of Microbiology 38:1–25
    [Google Scholar]
  4. Allen M.M., hawley M.A. 1983; Protein degradation and synthesis of cyanophycin granule polypeptide in Aphanocapsa sp. Journal of Bacteriology 154:1480–1484
    [Google Scholar]
  5. Allen M.M., Hutchison F. 1980; Nitrogen limitation and recovery in the cyanobacterium Aphanocapsa 6308. Archives of Microbiology 128:1–7
    [Google Scholar]
  6. Allen M.M., Smith A.J. 1969; Nitrogen chlorosis in blue-green algae. Archives of Microbiology 69:114–120
    [Google Scholar]
  7. Allen M.M., Hutchison F., Weathers P.J. 1980; Cyanophycin granule polypeptide formation and degradation in the cyanobacterium Aphanocapsa 6308. Journal of Bacteriology 141:687–693
    [Google Scholar]
  8. Bergersen F.J. 1980; Measurement of nitrogen fixation by direct means. In Methods for Evaluating Biological Nitrogen Fixation, pp 66–110 Bergersen F.J. Edited by Chichester: John Wiley;
    [Google Scholar]
  9. Boussiba S., Resch C.M., Gibson J. 1984; Ammonia uptake and retention in some cyanobacteria. Archives of Microbiology 138:287–292
    [Google Scholar]
  10. Carr N.G. 1983; Biochemical aspects of heterocyst differentiation and function. In Photosynthetic Prokaryotes: Cell Differentiation and Function pp 265–280 Papageorgiou G.C., Packer L. Edited by New York Amsterdam: Elsevier;
    [Google Scholar]
  11. Carr N.G. 1988; Nitrogen reserves and dynamic reservoirs in cyanobacteria. In Biochemistry of the Algae and Cyanobacteria (Annual Proceedings of the Phytochemical Society of Europe) pp 13–21 Rogers L.J., Gallon J.R. Edited by Oxford: Clarendon Press;
    [Google Scholar]
  12. Cohen D., Parnas H. 1976; An optimal policy for the metabolism of storage materials in unicellular algae. Journal of Theoretical Biology 56:1–18
    [Google Scholar]
  13. Daday A., Mackerras A.H., Smith G.D. 1988; A role for nickel in cyanobacterial nitrogen fixation and growth via cyanophycin metabolism. Journal of General Microbiology 134:2659–2663
    [Google Scholar]
  14. Dawes E.A., Senior P.J. 1973; The role and regulation of energy reserve polymers in micro-organisms. Advances in Microbial Physiology 10:135–266
    [Google Scholar]
  15. Flores E., Ramos J.L., Herrero A., Guerrero M.G. 1983; Nitrate assimilation by cyanobacteria. In Photosynthetic Prokaryotes: Cell Differentiation and Function pp 363–387 Papageorgiou G.C., Packer L. Edited by New York Amsterdam: Elsevier;
    [Google Scholar]
  16. Gupta M., Carr N.J. 1981; Enzyme activities related to cyanophycin metabolism in heterocysts and vegetative cells of Anabaena spp. Journal of General Microbiology 125:17–23
    [Google Scholar]
  17. Grillo J.F., Gibson J. 1979; Regulation of phosphate accumulation in the unicellular cyanobacterium Synechococcus. Journal of Bacteriology 140:508–517
    [Google Scholar]
  18. Koch A.L. 1988 Why can’t a cell grow infinitely fast? Canadian Journal of Microbiology 34:421–426
    [Google Scholar]
  19. Lawry N.H., Simon R.D. 1982; The normal and induced occurrence of cyanophycin inclusion bodies in several blue-green algae. Journal of Phycology 18:391–399
    [Google Scholar]
  20. Lehmann M., Wober G. 1976; Accumulation, mobilization and turn-over of glycogen in the blue-green bacterium Anacystis nidulans. Archives of Microbiology 111:93–97
    [Google Scholar]
  21. Mackerras A.H., Youens B.N., Weir R.C., Smith G.D. 1990; Is cyanophycin involved in the integration of nitrogen and carbon metabolism in the cyanobacteria Anabaena cylindrica and Gloeothece grown on light/dark cycles?. Journal of General Microbiology 136:2049–2056
    [Google Scholar]
  22. Meeks J.C., Wolk C.P., Thomas J., Lockau W., Shaffer P.W., Austin S.M., Chien W.-S., Galonsky A. 1977; The pathways of assimilation of 13NH+4 by the cyanobacterium, Anabaena cylindrica. Journal of Biological Chemistry 252:7894–7900
    [Google Scholar]
  23. Mur L.R. 1983; Some aspects of the ecophysiology of cyanobacteria. Annales de Microbiologie 134B:61–72
    [Google Scholar]
  24. Parnas H., Cohen D. 1976; The optimal strategy for the metabolism of reserve materials in micro-organisms. Journal of Theoretical Biology 56:19–55
    [Google Scholar]
  25. Rowell P., stewart W.D.P. 1976; Alanine dehydrogenase of the N2-fixing blue-green alga, Anabaena cylindrica. Archives of Microbiology 107:115–124
    [Google Scholar]
  26. Rowell P., Enticott S., Stewart W.D.P. 1977; Glutamine synthetase and nitrogenase activity in the blue-green alga Anabaena cylindrica. New Phytologist 79:41–54
    [Google Scholar]
  27. Schwinghamer E.A. 1980; A method for improved lysis of some gram-negative bacteria. FEMS Microbiology tetters 7:157–162
    [Google Scholar]
  28. Simon R.D. 1973; Measurement of the cyanophycin granule polypeptide contained in the blue-green alga Anabaena cylindrica. Journal of Bacteriology 114:1213–1216
    [Google Scholar]
  29. Simon R.D. 1976; The biosynthesis of multi-L-arginyl-poly- (L-aspartic acid) in the filamentous cyanobacterium Anabaena cylindrica. Biochimica et Biophysica Acta 422:407–418
    [Google Scholar]
  30. Simon R.D. 1987; Inclusion bodies in the cyanobacteria: cyanophycin, polyphosphate, polyhedral bodies. In The Cyanobacteria pp 199–225 Fay P., Van Baalen C. Edited by Amsterdam New York: Elsevier;
    [Google Scholar]
  31. Stevens S.E., Balkwill D.L., Paone D.A.M. 1981; a.The effects of nitrogen limitation on the ultrastructure of the cyanobacterium Agmenellum quadruplicatum. Archives of Microbiology 130:204–212
    [Google Scholar]
  32. Stevens S.E., Paone D.A.M., Balkwill D.L. 1981; b.Accumulation of cyanophycin granules as a result of phosphate limitation in Agmenellum quadruplicatum. Plant Physiology 67:716–719
    [Google Scholar]
  33. De Vasconcelos L., Fay P. 1974; Nitrogen metabolism and ultrastructure in Anabaena cylindrica. I.The effect of nitrogen starvation. Archives of Microbiology 96:271–279
    [Google Scholar]
  34. Wanner G., Henkelmann G., Schmidt A., Kost H.-P. 1986; Nitrogen and sulfur starvation of cyanobacterium Synechococcus 6301.An ultrastructural, morphometrical and biochemical comparison. Zeitschrift für Naturforschung 41 c:741–750
    [Google Scholar]
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