1887

Abstract

A hydrogenase operon was cloned from chromosomal DNA isolated from Miyazaki F with the use of probes derived from the genes encoding [NiFe] hydrogenase from Hildenborough. The nucleic acid sequence of the cloned DNA indicates this hydrogenase to be a two-subunit enzyme: the gene for the small subunit (267 residues; molecular mass = 28763 Da) precedes that for the large subunit (566 residues; molecular mass = 62495 Da), as in other [NiFe] and [NiFeSe] hydrogenase operons. The amino acid sequences of the small and large subunits of the Miyazaki hydrogenase share 80% homology with those of the [NiFe] hydrogenase from . Fourteen cysteine residues, ten in the small and four in the large subunit, which are thought to co-ordinate the iron-sulphur clusters and the active-site nickel in [NiFe] hydrogenases, are found to be conserved in the Miyazaki hydrogenase. The subunit molecular masses and amino acid composition derived from the gene sequence are very similar to the data reported for the periplasmic, membrane-bound hydrogenase isolated by Yagi and coworkers, suggesting that this hydrogenase belongs to the general class of [NiFe] hydrogenases, despite its low nickel content and apparently anomalous spectral properties.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-10-2021
1990-10-01
2021-05-10
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/10/mic-136-10-2021.html?itemId=/content/journal/micro/10.1099/00221287-136-10-2021&mimeType=html&fmt=ahah

References

  1. Bankier A.T., Barrell B.G. 1983; Shotgun DNA sequencing. In Techniques in the Life Sciences pp 1–34 Limerick, Ireland: Elsevier Scientific Publishers Ireland Ltd.;
    [Google Scholar]
  2. Bencze W.L., Schmid K. 1957; Determination of tyrosine and tryptophan in proteins. Analytical Chemistry 29:1193–1196
    [Google Scholar]
  3. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  4. Eidsness M.K., Scott R.A., Prickril B., Dervartanian D.V., Legall J., Moura I., Moura J.J.G., Peck H.D.Jr 1989; Evidence for selenocysteine coordination to the active site nickel in the [NiFeSe] hydrogenase from Desulfovibriobaculatus. Proceedings of the National Academy of Sciences of the United States of America 86:147–151
    [Google Scholar]
  5. Ford C.M., Garg N., Garg R.P., Tibelius K.H., Yates M.G., Arp D.J., Seefeldt L.C. 1990; The identification, characterization, sequencing and mutagenesis of the genes (hupSL) encoding the small and large subunits of the H2-uptake hydrogenase of Azotobacterchroococcum. Molecular Microbiology 4: in the Press
    [Google Scholar]
  6. Gow L.A., Pankhania I.P., Ballantine S.P., Boxer D.H., Hamilton W.A. 1986; Identification of a membrane-bound hydrogenase of Desulfovibrio vulgaris (Hildenborough). Biochimica et BiophysicaActa 851:57–64
    [Google Scholar]
  7. Hatchikian E.C., Bruschi M., Legall J. 1978; Characterization of the periplasmichydrogenase from Desulfovibriogigas. Biochemical and Biophysical Research Communications 82:451–461
    [Google Scholar]
  8. Higuchi Y., Yasuoka N., Kakudo M., Katsube Y., Yagi T., Inokuchi H. 1987; Single crystals of hydrogenase from Desulfovibrio vulgaris Miyazaki F. Journal of Biological Chemistry 262:2823–2825
    [Google Scholar]
  9. Kissinger C.R., Adman E.T., Sieker L.C., Jensen L.H. 1989; The crystal structure of the three-iron ferredoxin II from D. gigas. FEBS Letters 242:447–450
    [Google Scholar]
  10. Leclerc M., Colbeau A., Cauvin B., Vignais P.M. 1988; Cloning and sequencing of the genes encoding the large and the small subunits of the H2 uptake hydrogenase(hup) of Rhodobactercapsulatus. Molecular and General Genetics 214:97–108
    [Google Scholar]
  11. Li C., Peck H.D.Jr Legall J., Przybyla A.E. 1987; Cloning, characterization and sequencing of the genes encoding the large and small subunits of the periplasmic [NiFe] hydrogenase of Desulfovibriogigas. DNA 6:539–551
    [Google Scholar]
  12. Lissolo T., Choi E.S., Legall J., Peck H.D.Jr 1986; The presence of multiple intrinsic membrane nickel containing hydrogenase in Desulfovibrio vulgaris (Hildenborough). Biochemical and Biophysical Research Communications 139:701–708
    [Google Scholar]
  13. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  14. Menon N.K., Peck H.D.Jr. Legall J., Przybyla A.E. 1987; Cloning and sequencing of the genes encoding the large and small subunits of the periplasmic (NiFeSe) hydrogenase of Desulfovibriobaculatus. Journal of Bacteriology 169:5401–5407
    [Google Scholar]
  15. Menon N.K., Robbins J., Peck H.D.Jr. Chatelus C.Y., Choi E.-S., Przybyla A.E. 1990; Cloning and sequencing of a putative Escherichia coli [NiFe] hydrogenase-1 operon containing six open reading frames. Journal of Bacteriology 172:1969–1977
    [Google Scholar]
  16. Messing J., Vieira J. 1982; A new pair of of M13 vectors for selecting either DNA strand of double digest restriction fragments. Gene 19:269–276
    [Google Scholar]
  17. Moura J.J.G., Moura I., Huynh B.-H., Kruger H.-J., Texeira M., Duvarney R.C., Dervartanian D.V., Xavier A.V., Peck H.D.Jr LeGall J. 1982; Unambiguous identification of the nickel EPR signal in 61 Ni enriched Desulfovibriogigashydrogenase. Biochemical and Biophysical Research Communications 108:1388–1393
    [Google Scholar]
  18. Niviere V., Forget N., Bovier-Lapierre G., Bonicel J., Hatchikian C. 1988; Isolation, amino acid analysis and N-terminal sequence determination of the two subunits of the nickel-containing hydrogenase of Desulfovibriogigas. Biochimie 70:267–271
    [Google Scholar]
  19. Prickril B.C., Czechowsi M.H., Przybyla A.E., Peck H.D.JR. 1986; Putative signal peptide on the small subunit of the periplasmichydrogenase from Desulfovibrio vulgaris. Journal of Bacteriology 167:722–725
    [Google Scholar]
  20. Sanger F., Nicklen S., coulson A.R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 74:5463–5467
    [Google Scholar]
  21. Sayavedra-SOTO L.A., Powell G.K., Evans H.J., Morris R.O. 1988; Nucleotide sequence of the genetic loci encoding subunits of Bradyrhizobiumjaponicum uptake hydrogenase. Proceedings of the National Academy of Sciences of the United States of America 85:8395–8399
    [Google Scholar]
  22. Staden R. 1982; Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing. Nucleic Acids Research 10:4731–4751
    [Google Scholar]
  23. Staden R. 1984; a.A computer program to determine the function of nucleic acid sequences. Nucleic Acids Research 12:499–503
    [Google Scholar]
  24. Staden R. 1984; b. Graphic methods to determine the function of nucleic acid sequences. Nucleic Acids Research 12:521–538
    [Google Scholar]
  25. Staden R., Mclachlan A.D. 1982; Codon preference and its use in identifying protein coding regions in long DNA sequences. Nucleic Acids Research 10:141–156
    [Google Scholar]
  26. Vandongen W., Kaan A., Vandenberg W., Veeger C. 1989; hydy, a gene from Desulfovibrio vulgaris (Hildenborough) encodes a polypeptide homologous to the periplasmichydrogenase. FEMS Microbiology Letters 58:217–222
    [Google Scholar]
  27. Tamura A., Kawate T., Ogata M., Yagi T. 1988; Interaction of cellular hydrogenase, cytochrome c3 and desulfoviridin in Desulfovibrio vulgaris Miyazaki with their antibodies. Journal of Biochemistry 104:722–726
    [Google Scholar]
  28. Uffen R.L., Colbeau A., Richaud P., Vignais P.M. 1990; Cloning and sequencing the genes encoding uptake-hydrogenase subunits of Rhodocyclusgelatinosus. Molecular and General Genetics 221:49–58
    [Google Scholar]
  29. Vieira J., Messing J. 1982; The pUC plasmids, an M13mp7- derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
    [Google Scholar]
  30. Voordouw G., Brenner S. 1985; Nucleotide sequence of the gene encoding the hydrogenase from Desulfovibrio vulgaris (Hildenborough). European Journal of Biochemistry 148:515–520
    [Google Scholar]
  31. Voordouw G., Walker J.E., Brenner S. 1985; Cloning of the gene encoding the hydrogenase from Desulfovibrio vulgaris (Hildenborough) and determination of the NH2-terminal sequence. European Journal of Biochemistry 148:509–514
    [Google Scholar]
  32. Voordouw G., Hagen W.R., Kruse-WOLTERS M., Vanberkel-ARTS A., Veeger C. 1987; Purification and characterization of Desulfovibrio vulgaris (Hildenborough) hydrogenase expressed in Escherichia coli. European Journal of Biochemistry 162:31–36
    [Google Scholar]
  33. Voordouw G., Menon N.K., Legall J., Choi E.-S., Peck H.D.JR. 1989a.; Analysis and comparison of nucleotide sequences encoding the genes for [NiFe] and [NiFeSe] hydrogenase from Desulfovibriogigas and Desulfovibriobaculatus. Journal of Bacteriology 171:2894–2899
    [Google Scholar]
  34. Voordouw G., Strang J.D., Wilson F.R. 1989b; Organization of the genes encoding [Fe] hydrogenase in Desulfovibrio vulgaris subsp. oxamicus Monticello. Journal of Bacteriology 171:3881–3889
    [Google Scholar]
  35. Yagi T. 1970; Solubilization, purification and properties of particulate hydrogenase from Desulfovibrio vulgaris. Journal of Biochemistry 68:649–657
    [Google Scholar]
  36. Yagi T., Kimura K., Daidoji H., Sakai F., Tamura S., Inokuchi H. 1976; Properties of purified hydrogenase from the particulate fraction of Desulfovibrio vulgaris Miyazaki. Journal of Biochemistry 79:661–671
    [Google Scholar]
  37. Yagi T., Kimura K., Inokuchi H. 1985; Analysis of the active centre of hydrogenase from Desulfovibrio vulgaris Miyazaki by magnetic measurements. Journal of Biochemistry 97:181–187
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-10-2021
Loading
/content/journal/micro/10.1099/00221287-136-10-2021
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error