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Mutants of the obligatorily aerobic yeast Rhodotorula glutinis, obtained by treating wild-type cells with N-methyl-N′-nitro-N-nitrosoguanidine, were selected on fructose medium containing 1% 2-deoxy-d-glucose. One mutant, designated R33, displayed a significantly decreased initial rate of uptake of d-glucose, 2-deoxy-d-glucose, d-galactose and d-xylose, and was unable to accumulate the latter three sugars. However, the residual monosaccharide transport was still energy-dependent. The mutant displayed significantly altered transport parameters for d-glucose and d-xylose. These results are evidence for the isolation of a mutant defective in the glucose carrier.
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