Inhibition of the dimorphic transition of Candida albicans by the ornithine decarboxylase inhibitor 1,4-diaminobutanone: alterations in the glycoprotein composition of the cell wall
Hyphal development in Candida albicans was selectively blocked by the ornithine decarboxylase competitive inhibitor 1,4-diaminobutanone (DAB). Inhibition of hyphal development required DAB during both yeast inoculum growth and subsequent incubation at 37°C to induce mycelial growth. This effect was not due to general growth inhibition since DAB did not inhibit yeast growth, and reduced protein synthesis by 30% at most. Moreover, protein synthesis was unaffected by DAB when cells were pre-grown in drug-containing media. Since DAB inhibited dimorphic transition at 37°C, morphology-and temperature-dependent protein synthesis could be distinguished. DAB stimulated the synthesis of several yeast wall-proteins, irrespective of morphology or growth temperature, and two at 37°C only, but it inhibited the synthesis of a single mycelial-specific glycoprotein species.
AndersonM.L., OddsF.C.1985; Adherence of Candida albicans to vaginal epithelia: significance of morphological form and effect of ketoconazole. Mykosen 28:2035–2047
DuboisM., GillesK.A., HamiltonJ.K., RebersP.A., SmithF.1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
ElorzaM.V., MurguiA., SentandreuR.1985; Dimorphism in Candida albicans : contribution of mannoproteins to the architecture of yeast and mycelial cell walls. Journal of General Microbiology 131:2209–2216
ElorzaM.V., MormeneoS., Garcia De La CruzF., GimenoC., SentandreuR.1989; Evidence for the formation of covalent bonds between macromolecules in the domain of the wall of Candida albicans mycelial cells. Biochemical and Biophysical Research Communications 162:1118–1125
FarrelS.M., HawkinsD.F., RyderT.A.1983; Scanning electron microscope study of Candida albicans invasion of cultured human cervical epithelial cells. Sabouraudia 21:251–254
InderliedC.B., CihlarR.L., SypherdP.S.1980; Regulation of ornithine decarboxylase during morphogenesis of Mucor racemosus. Journal of Bacteriology 141:699–706
KimW.K.1971; Folate and polyamine content of indifferentiated and differentiated wheat stem rust uredosporelings. Canadian Journal of Botany 49:1119–1122
KimuraL.H., PearsallN.N.1980; Relationship between germination of Candida albicans and increased adherence to human buccal epithelial cells. Infection and Immunity 28:464–468
LeeK.L., BuckleyH.R., CampbellC.1975; An amino acid liquid synthetic medium for development of mycelial and yeast forms of Candida albicans. Sabouraudia 13:148–153
MartinezJ.P., GilM.L., CasanovaM., RicoH., SentandreuR., Ruiz-HerreraJ.1989; Characterization of a proteinaceous extracellular coat synthesized by the ‘slime’ variant of Neurospora crassa. Archives of Microbiology 152:25–32
Martinez-PachecoM., RodriguezG., ReynaG., Calvo-MendezC., Ruiz-HerreraJ.1989; Inhibition of the yeast-mycelial transition and the phorogenesis of Mucorales by diamino-butanone. Archives of Microbiology 151:10–14
MennucciL., RojasS., CamargoE.P.1975; Polyamines and ornithine decarboxylase activity during growth and differentiation in Blastocladiella emersonii. Biochimica et Biophysica Acta 404:249–256
Ruiz-HerreraJ., Calvo-MendezC.1987; Effect of ornithine decarboxylase inhibitors on the germination of sporangiospores of Mucorales. Experimental Mycology 11:287–296
SmithC.B.1985; Candidiasis: pathogenesis, host resistance and predisposing factors. In Candidiasis pp 53–70BodeyG. P., FainsteinV. Edited by New York: Raven Press;
SobelJ.D., ObedeanuN.1983; Effects of subinhibitory concentrations of ketoconazole on in vitro adherence of Candida albicans to vaginal epithelial cells. European Journal of Clinical Microbiology 2:445–452
SollD.R.1985; Candida albicans. In Fungal Dimorphism: with Emphasis on Fungi Pathogenic to Humans pp 167–195SzanisloP.J. Edited by New York: Plenum Publishing Co;
StevensL., MckinnonI.M., WintherM.D.1976; Polyamine metabolism during the germination of conidia of Aspergillus nidulans. Biochemical Journal 158:235–241
SundstromP.M., NicholsE.J., KennyG.E.1987; Antigenic differences between mannoproteins of germ tubes and blastospores of Candida albicans. Infection and Immunity 55:616–620
Inhibition of the dimorphic transition of Candida albicans by the ornithine decarboxylase inhibitor 1,4-diaminobutanone: alterations in the glycoprotein composition of the cell wall