SUMMARY: The structure and location of nopaline catabolism (noc) genes were examined in various strains by plasmid transfer and by Southern hybridization analysis. In a pathogenic strain, PyTS3, the genes were identified both on the Ti-plasmid, pTiPyTS3, and on the cryptic plasmid, pCr140. Plasmid pCr140 was transmitted to another strain at a higher frequency than was pTiPyTS3. Southern hybridization analysis, using Ti-plasmid-derived genes as a probe, indicated that the genes of pTiPyTS3 differed structurally from those of pCr140. Three non-pathogenic strains possessed plasmids that hybridized to the probe, and all showed identical patterns of hybridization after restriction enzyme cleavage. However, this pattern was different from those of pTiPyTS3 and pCr140. Only pCr140 showed partial hybridization to the probe. A nonpathogenic strain, PyON8, that grew on both nopaline and octopine, showed no hybridization of its plasmid or chromosome to the probe. Since nopaline-utilizing transconjugants were not obtained from PyON8, the genes in PyON8 may be chromosomally located. These results indicate that contains diverse genes which differ in both organization and cellular localization.


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