1887

Abstract

The structure and location of nopaline catabolism (noc) genes were examined in various strains by plasmid transfer and by Southern hybridization analysis. In a pathogenic strain, PyTS3, the genes were identified both on the Ti-plasmid, pTiPyTS3, and on the cryptic plasmid, pCr140. Plasmid pCr140 was transmitted to another strain at a higher frequency than was pTiPyTS3. Southern hybridization analysis, using Ti-plasmid-derived genes as a probe, indicated that the genes of pTiPyTS3 differed structurally from those of pCr140. Three non-pathogenic strains possessed plasmids that hybridized to the probe, and all showed identical patterns of hybridization after restriction enzyme cleavage. However, this pattern was different from those of pTiPyTS3 and pCr140. Only pCr140 showed partial hybridization to the probe. A nonpathogenic strain, PyON8, that grew on both nopaline and octopine, showed no hybridization of its plasmid or chromosome to the probe. Since nopaline-utilizing transconjugants were not obtained from PyON8, the genes in PyON8 may be chromosomally located. These results indicate that contains diverse genes which differ in both organization and cellular localization.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-1-97
1990-01-01
2021-08-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/1/mic-136-1-97.html?itemId=/content/journal/micro/10.1099/00221287-136-1-97&mimeType=html&fmt=ahah

References

  1. Akiyoshi D. E., Klee H., Amasino , Nester E. W., Gordon M. P. 1984; T-DNA of Agrobacterium tumefaciens encodes an enzyme of cytokinin biosynthesis. Proceedings of the National Academy of Sciences of the United States of America 81:5994–5998
    [Google Scholar]
  2. Bomhoff G., Klapwijk P. M., Kester H.C.M., Schilperoort R. A. 1976; Octopine and nopaline synthesis and breakdown genetically controlled by a plasmid of Agrobacterium tumefaciens. Molecular and General Genetics 145:177–181
    [Google Scholar]
  3. Chilton M.-D., Currier T. C., Farrand S. L., Bendich A. J., Gordon M. P., Nester E. W. 1974; Agrobacterium tumefaciensDNA and PS8 bacteriophage DNA not detected in crown gall tumors. Proceedings of the National Academy of Sciences of the United States of America 71:3672–3676
    [Google Scholar]
  4. Chilton M.-D., Drummond M. H., Sciaky D., Montoya A. L., Gordon M. P., Nester E. W. 1977; Stable incorporation of plasmid DNA into higher plant cells. Cell 11:263–271
    [Google Scholar]
  5. Currier T. C., Nester E. W. 1976; Isolation of covalently closed circular DNA of high molecular weight from bacteria. Analytical Biochemistry 76:431–441
    [Google Scholar]
  6. Depicker A., De Wilde M., De Vos G., De Vos R., Van Montagu M., Schell J. 1980; Molecular cloning of overlapping segments of the nopaline Ti-plasmid pTiC58 as a means to restriction endonuclease mapping. Plasmid 3:193–211
    [Google Scholar]
  7. Ellis J. G., Kerr A. 1979; Arginine catabolism: a new function of both octopine and nopaline Ti-plasmids of Agrobacterium. Molecular and General Genetics 173:263–269
    [Google Scholar]
  8. Ellis J. G., Kerr A., Petit A., Tempe J. 1982; Conjugal transfer of nopaline and agropine Ti plasmids - the role of agrocinopines. Molecular and General Genetics 186:269–274
    [Google Scholar]
  9. Holsters M., De Waele D., Depicker A., Messens E., Van Montagu M., Schell J. 1978; Transfection and transformation of Agrobacterium tumefaciens. Molecular and General Genetics 163:181–187
    [Google Scholar]
  10. Holsters M., Silva B., Van Vliet F., Genetello C., De Block M., Dhaese P., Depicker A., Inze D., Engler G., Villaroel R., Van Montagu M., Schell J. 1980; The functional organization of the nopalineA. tumefaciens plasmid pTiC58. Plasmid 3:212–230
    [Google Scholar]
  11. Hooykaas P.J.J., Dendulk-Ras H., Ooms G., Schilperoort R. A. 1980; Interactions between octopine and nopaline plasmids in Agrobacterium tumefaciens. Journal of Bacteriology 143:1295–1306
    [Google Scholar]
  12. Kado C. I., Liu S.-T. 1981; Rapid procedure for detection and isolation of large and small plasmids. Journal of Bacteriology 145:1365–1373
    [Google Scholar]
  13. Klapwijk P. M., Scheulderman T., Schilperoort R. A. 1978; Coordinated regulation of octopine degradation and conjugative transfer of Ti plasmids in Agrobacterium tumefaciens : evidence for a common regulatory gene and separate operons. Journal of Bacteriology 136:775–785
    [Google Scholar]
  14. Lichtenstein C., Draper J. 1985; Genetic engineering of plants. In DNA Cloning, 2 pp 67–119 Glover D. M. Edited by Washington, DC: IRL Press;
    [Google Scholar]
  15. Machida Y., Sakurai M., Kiyokawa S., Ubasawa A., Suzuki Y., Ikeda J. 1984; Nucleotide sequence of the insertion sequence found in the T-DNA region of mutant Ti plasmid pTiA66 and distribution of its homologues in octopine Ti plasmid. Proceedings of the National Academy of Sciences of the United States of America 81:7495–7499
    [Google Scholar]
  16. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Merlo D. J., Nester E. W. 1977; Plasmids in avirulent strains of Agrobacterium. Journal of Bacteriology 129:76–80
    [Google Scholar]
  18. Montoya A. L., Moore L. W., Gordon M. P., Nester E. W. 1978; Multiple genes coding for octopine-degrading enzymes in Agrobacterium. Journal of Bacteriology 136:909–915
    [Google Scholar]
  19. Nester E. W., Gordon M. P., Amasino R. M., Yanofsky M. F. 1984; Crown gall: a molecular and physiological analysis. Annual Review of Plant Physiology 35:387–413
    [Google Scholar]
  20. Petit A., Tempe J. 1978; Isolation of Agrobacterium Ti-plasmid regulatory mutants. Molecular and General Genetics 167:147–155
    [Google Scholar]
  21. Petit A., David C., Dahl G. A., Ellis J. G., Guyon P. 1983; Further extension of the opine concept: plasmids in Agrobacterium rhizogenes cooperate for opine degradation. Molecular and General Genetics 190:204–214
    [Google Scholar]
  22. Schardl C. L., Kado C. I. 1983; A functional map of the nopaline catabolism genes on the Ti-plasmid of Agrobacterium tumefaciens C58. Molecular and General Genetics 191:10–16
    [Google Scholar]
  23. Sciaky D., Montoya A. L., Chilton M.-D. 1978; Fingerprints of Agrobacterium Ti plasmids. Plasmid 1:238–253
    [Google Scholar]
  24. Slota J. E., Farrand S. K. 1982; Genetic isolation and physical characterization of pAgK84, the plasmid responsible for agrocin 84 production. Plasmid 8:175–186
    [Google Scholar]
  25. Thomashow L. S., Reeves S., Thomashow M. F. 1984; Crown gall oncogenesis: evidence that a T-DNA gene from the Agrobacterium Ti plasmid pTiA6 encodes an enzyme that catalyses synthesis of indoleacetic acid. Proceedings of the National Academy of Sciences of the United States of America 81:5071–5075
    [Google Scholar]
  26. Unger L., Ziegler S. F., Huffman G. A., Knauf V. C., Peet R., Moore L. W., Gordon M. P., Nester E. W. 1985; New class of limited-host-range Agrobacterium mega-tumor-inducing plasmids lacking homology to the transferred DNA of a wide-host-range, tumor-inducing plasmid. Journal of Bacteriology 164:723–730
    [Google Scholar]
  27. Van Larebeke N., Engler G., Holsters M., Van Den Elsacker S., Zaenen I., Shilperpoort R. A., Schell J. 1974; Large plasmid in Agrobacterium tumefaciens is essential for crown gall-inducing ability. Nature, London 252:169–170
    [Google Scholar]
  28. Wabiko H., Kagaya M., Kodama I., Masuda K., Kodama Y., Yamamoto H., Shibano Y., Sano H. 1989; Isolation and characterization of diverse nopaline type Ti plasmids of Agrobacterium tumefaciens from Japan. Archives of Microbiology 152:119–124
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-1-97
Loading
/content/journal/micro/10.1099/00221287-136-1-97
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error