Enzymes for biosynthesis and elongation of fatty acids in mycobacteria grown in host cells: is competent in fatty acid biosynthesis? Free

Abstract

Fatty acid synthetase activity in extracts of was equivalent to 1·7 pmol malonyl-CoA incorporated into fatty acid min (mg protein). This activity - if representative of living organisms - is insufficient to enable them to synthesize their lipid requirements rapidly enough to support growth. The major activity for scavenging fatty acids in extracts of and , as well as in extracts of , was acetyl-CoA-dependent fatty acyl-CoA ‘elongase’. This activity was about four times higher in and grown in a medium which contained lipids, or when grown in mice, than in medium without added lipids. In contrast, the fatty acid synthetase activity was repressed in and when grown in medium that contained lipids, or when grown in mice. These results are consistent with the hypothesis that mycobacteria grown preferentially scavenge lipids from the host cells, and suggest that a source of lipid should be included in media for attempted axenic isolation of

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1990-01-01
2024-03-28
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References

  1. Ascenzi J. M., Vestal V. R. 1979; Regulation of fatty acid biosynthesis by carbon substrates in Mycobacterium convolutum. . Journal of Bacteriology 137:384–390
    [Google Scholar]
  2. Barclay R., Wheeler P. R. 1989; Metabolism of mycobacteria in tissues. In The Biology of the Mycobacteria, 3 pp 37–106 Ratledge C., Stanford J., Grange J. M. Edited by London: Academic Press;
    [Google Scholar]
  3. Bloch K. 1975; Fatty acid synthetases from Mycobacterium phlei. . Methods in Enzymology 35:85–90
    [Google Scholar]
  4. Bloch K. 1977; Control mechanisms for fatty acid synthesis in Mycobacterium smegmatis. . Advances in Enzymology 45:1–84
    [Google Scholar]
  5. Chadwick M. V. 1982; Mycobacteria. Institute of Medical Laboratory Sciences Monographs. Bristol:: P. S. G. Wright.;
    [Google Scholar]
  6. Gillin F. D., Gaulton J., Hoffmann A. F., Gurantz D., Sauch J. F. 1986; Biliary lipids support serum-free growth of Giardia lamblia. . Infection and Immunity 53:641–645
    [Google Scholar]
  7. Kondo E., Suzuki K., Kanai K., Yasuda T. 1985; Liposomes- mycobacteria incubation systems as a partial model of host-parasite interaction at cell membrane level. Japanese Journal of Medical Science and Biology 38:169–180
    [Google Scholar]
  8. Kusaka T. 1977; Fatty acid synthesizing enzyme activity of cultured Mycobacterium lepraemurium. . International Journal of Leprosy 45:132–144
    [Google Scholar]
  9. Levy L. 1976; Studies in the mouse footpad technique for cultivation of Mycobacterium leprae. 3. Doubling time during logarithmic multiplication. Leprosy Review 47:103–106
    [Google Scholar]
  10. Medhi J., Murthy P. S., Venkitasubramanian T. A. 1979; Demonstration and purification of three fatty acid synthetases from Mycobacterium tuberculosis H37RV. Indian Journal of Biochemistry and Biophysics 16:216–222
    [Google Scholar]
  11. Minnikin D. E. 1982; Lipids: complex lipids, their chemistry, biosynthesis and roles. In The Biology of the Mycobacteria 1 pp 95–184 Ratledge C., Stanford J. Edited by London: Academic Press;
    [Google Scholar]
  12. Mor N. 1983; Intracellular location of Mycobacterium leprae in macrophages of normal and immunodeficient mice and effect of rifampicin. Infection and Immunity 42:802–811
    [Google Scholar]
  13. Mukherjee R. M., Antia N. H. 1985; Intracellular multiplication of leprosy-derived mycobacteria in Schwann cells of dorsal root ganglion cultures. Journal of Clinical Microbiology 21:208–212
    [Google Scholar]
  14. Peterson K. M., Alderete J. F. 1984; Trichomonas vaginalis is dependent on uptake and degradation of human low density lipoproteins. Journal of Experimental Medicine 160:1261–1272
    [Google Scholar]
  15. Ratledge C. 1982; Lipids: cell composition, fatty acid biosynthesis. In The Biology of the Mycobacteria, 1 pp 53–93 Ratledge C., Stanford J. Edited by London: Academic Press;
    [Google Scholar]
  16. Saito H., Tomioka H., Yoneyama T. 1984; Growth of Group IV mycobacteria on medium containing various saturated and unsaturated fatty acids. Antimicrobial Agents and Chemotherapy 26:164–169
    [Google Scholar]
  17. Wheeler P. R. 1987a; Biosynthesis and scavenging of purines by pathogenic mycobacteria including Mycobacterium leprae. . Journal of General Microbiology 133:2999–3011
    [Google Scholar]
  18. Wheeler P. R. 1987b; Enzymes for purine synthesis and scavenging in pathogenic mycobacteria and their distribution in Mycobacterium leprae. . Journal of General Microbiology 133:3013–3018
    [Google Scholar]
  19. Wheeler P. R., Gregory D. 1980; Superoxide dismutase, peroxidatic activity and catalase in Mycobacterium leprae purified from armadillo liver. Journal of General Microbiology 121:457–464
    [Google Scholar]
  20. Wheeler P. R., Ratledge C. 1988; Use of carbon sources for lipid biosynthesis in Mycobacterium leprae: a comparison with other pathogenic mycobacteria. Journal of General Microbiology 134:2111–2121
    [Google Scholar]
  21. Wheeler P. R., Bharadwaj V. P., Gregory D. 1982; N-Acetyl- β -glucosaminidase, β -glucoronidase and acid phosphatase in Mycobacterium leprae. . Journal of General Microbiology 128:1063–1071
    [Google Scholar]
  22. World Health Organization 1980 UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases. Report of the Fifth Meeting on the Immunology of Leprosy (IMMLEP). TDRjIMMLEP-SWG (5)/80.3, Annex 4, p. 23. Geneva:: World Health Organization.;
    [Google Scholar]
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