Enzymes for biosynthesis de novo and elongation of fatty acids in mycobacteria grown in host cells: is Mycobacterium leprae competent in fatty acid biosynthesis?
Fatty acid synthetase activity in extracts of Mycobacterium leprae was equivalent to 1·7 pmol malonyl-CoA incorporated into fatty acid min-1 (mg protein)-1. This activity - if representative of living M. leprae organisms - is insufficient to enable them to synthesize their lipid requirements rapidly enough to support growth. The major activity for scavenging fatty acids in extracts of Mycobacterium microti and Mycobacterium avium, as well as in extracts of M. leprae, was acetyl-CoA-dependent fatty acyl-CoA ‘elongase’. This activity was about four times higher in M. avium and M. microti grown in a medium which contained lipids, or when grown in mice, than in medium without added lipids. In contrast, the de novo fatty acid synthetase activity was repressed in M. avium and M. microti when grown in medium that contained lipids, or when grown in mice. These results are consistent with the hypothesis that mycobacteria grown in vivo preferentially scavenge lipids from the host cells, and suggest that a source of lipid should be included in media for attempted axenic isolation of M. leprae.
AscenziJ. M.,
VestalV. R.1979; Regulation of fatty acid biosynthesis by carbon substrates in Mycobacterium convolutum.
. Journal of Bacteriology 137:384–390
BarclayR.,
WheelerP. R.1989; Metabolism of mycobacteria in tissues. In The Biology of the Mycobacteria,3 pp 37–106RatledgeC.,
StanfordJ.,
GrangeJ. M.
Edited by London: Academic Press;
KondoE.,
SuzukiK.,
KanaiK.,
YasudaT.1985; Liposomes- mycobacteria incubation systems as a partial model of host-parasite interaction at cell membrane level. Japanese Journal of Medical Science and Biology 38:169–180
LevyL.1976; Studies in the mouse footpad technique for cultivation of Mycobacterium leprae. 3. Doubling time during logarithmic multiplication. Leprosy Review 47:103–106
MedhiJ.,
MurthyP. S.,
VenkitasubramanianT. A.1979; Demonstration and purification of three fatty acid synthetases from Mycobacterium tuberculosis H37RV. Indian Journal of Biochemistry and Biophysics 16:216–222
MinnikinD. E.1982; Lipids: complex lipids, their chemistry, biosynthesis and roles. In The Biology of the Mycobacteria1 pp 95–184RatledgeC.,
StanfordJ.
Edited by London: Academic Press;
MorN.1983; Intracellular location of Mycobacterium leprae in macrophages of normal and immunodeficient mice and effect of rifampicin. Infection and Immunity 42:802–811
MukherjeeR. M.,
AntiaN. H.1985; Intracellular multiplication of leprosy-derived mycobacteria in Schwann cells of dorsal root ganglion cultures. Journal of Clinical Microbiology 21:208–212
PetersonK. M.,
AldereteJ. F.1984; Trichomonas vaginalis is dependent on uptake and degradation of human low density lipoproteins. Journal of Experimental Medicine 160:1261–1272
RatledgeC.1982; Lipids: cell composition, fatty acid biosynthesis. In The Biology of the Mycobacteria,1 pp 53–93RatledgeC.,
StanfordJ.
Edited by London: Academic Press;
SaitoH.,
TomiokaH.,
YoneyamaT.1984; Growth of Group IV mycobacteria on medium containing various saturated and unsaturated fatty acids. Antimicrobial Agents and Chemotherapy 26:164–169
WheelerP. R.1987a; Biosynthesis and scavenging of purines by pathogenic mycobacteria including Mycobacterium leprae.
. Journal of General Microbiology 133:2999–3011
WheelerP. R.1987b; Enzymes for purine synthesis and scavenging in pathogenic mycobacteria and their distribution in Mycobacterium leprae.
. Journal of General Microbiology 133:3013–3018
WheelerP. R.,
GregoryD.1980; Superoxide dismutase, peroxidatic activity and catalase in Mycobacterium leprae purified from armadillo liver. Journal of General Microbiology 121:457–464
WheelerP. R.,
RatledgeC.1988; Use of carbon sources for lipid biosynthesis in Mycobacterium leprae: a comparison with other pathogenic mycobacteria. Journal of General Microbiology 134:2111–2121
WheelerP. R.,
BharadwajV. P.,
GregoryD.1982; N-Acetyl- β-glucosaminidase, β-glucoronidase and acid phosphatase in Mycobacterium leprae.
. Journal of General Microbiology 128:1063–1071
World Health Organization1980UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases. Report of the Fifth Meeting on the Immunology of Leprosy (IMMLEP). TDRjIMMLEP-SWG (5)/80.3, Annex 4, p. 23. Geneva:: World Health Organization.;
Enzymes for biosynthesis de novo and elongation of fatty acids in mycobacteria grown in host cells: is Mycobacterium leprae competent in fatty acid biosynthesis?