1887

Abstract

P NMR spectra were obtained from perchloric acid (PCA) and KOH extracts of mycelium. Signals indicating the presence of large amounts of short-chain polyphosphate were observed in the spectra of PCA extracts of mycelia grown under both low (0·1 m) and high (10 m) phosphate conditions. The mean chain length of polyphosphate was calculated from the relative areas of signals arising from terminal and internal P nuclei in the polyphosphate chain. The small amount of polyphosphate evident in the KOH extract had an average chain length similar to PCA-soluble polyphosphate. P tracer studies indicated that phosphorus in the PCA fraction accounted for between 50 and 60% of total phosphorus, the bulk of the remainder being divided between the lipid and KOH extracts. The presence of the fungicide phosphorous acid markedly reduced the average chain length of acid-soluble polyphosphate. This reduction occurred both under low-phosphate conditions, in which treatment with phosphorous acid retards growth, and under high-phosphate conditions, in which no significant growth retardation is observed. Treatment with phosphorous acid perturbed phosphorus distribution and lipid composition under low-phosphate conditions.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-1-147
1990-01-01
2021-07-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/1/mic-136-1-147.html?itemId=/content/journal/micro/10.1099/00221287-136-1-147&mimeType=html&fmt=ahah

References

  1. Bligh E. G., Dyer W. J. 1959; A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 37:911–917
    [Google Scholar]
  2. Bostock R. M., Creamer J. R. 1986; Characterization and biological activity of Phytophthora infestans phospholipids in the hypersensitive response of potato tuber. Physiological and Molecular Plant Pathology 28:215–225
    [Google Scholar]
  3. Bower L. A., Coffey M. D. 1985; Development of laboratory tolerance to phosphorous acid, Fosetyl-Al and Metalaxyl in Phytophthora capsici. Canadian Journal of Plant Pathology 7:1–6
    [Google Scholar]
  4. Cassone A., Carpinelli G., Angiolella L., Maddaluno G., Podo F. 1983; 31P nuclear magnetic resonance study of growth and dimorphic transition in Candida albicans. Journal of General Microbiology 129:1569–1575
    [Google Scholar]
  5. Chilvers G. A., Lapeyrie F. F., Douglass P. A. 1985; A contrast between oomycetes and other taxa of mycelial fungi in regard to metachromatic granule formation. New Phytologist 99:203–210
    [Google Scholar]
  6. Coffey M. D., Bower L. A. 1984; In vitro variability of eight Phytophthora species in response to phosphorous acid. Phytopathology 74:738–741
    [Google Scholar]
  7. Coffey M. D., Joseph M. C. 1985; Effects of phosphorous acid and Fosetyl-AI in the life cycle of Phytophthora cinnamomi and P. citricola. Phytopathology 75:1042–1045
    [Google Scholar]
  8. Cohen Y., Coffey M. D. 1986; Systemic fungicides and the control of Oomycetes. Annual Review of Phytopathology 24:311–338
    [Google Scholar]
  9. Dercks W., Buchenauer H. 1987; Comparative studies on the mode of action of aluminium ethyl phosphite in four Phytophthora species. Crop Protection 6:82–89
    [Google Scholar]
  10. Deslauriers R., Ekiel I., Byrd R. A., Jarrell H. C., Smith I.C.P. 1982; A 31P-NMR study of structural and functional aspects of phosphate and phosphonate distribution in Tetrahymena. Biochimica et Biophysica Acta 720:329–337
    [Google Scholar]
  11. Dietrich S.M.C. 1976; Presence of polyphosphate of low molecular weight in Zygomycetes. Journal of Bacteriology 127:1408–1413
    [Google Scholar]
  12. Dittmer J. C., Lester R. L. 1964; A simple specific spray for the detection of phospholipids on thin layer chromatograms. Journal of Lipid Research 5:126–127
    [Google Scholar]
  13. Dolan T. E., Coffey M. D. 1988; Correlative in vitro and in vivo behaviour of mutant strains of Phytophthora palmivoraexpressing different resistances to phosphorous acid and fosetyl-Al. Phytopathology 78:974–978
    [Google Scholar]
  14. Fenn M. E., Coffey M. D. 1984; Studies on the in vitro and in vivo antifungal activity of Fosetyl-Al and phosphorous acid. Phytopathology 74:606–611
    [Google Scholar]
  15. Glonek T., Lunde M., Mudgett M., Myers T. C. 1971; Studies of biological polyphosphate through the use of phosphorus-31 nuclear magnetic resonance. Archives of Biochemistry and Biophysics 142:508–513
    [Google Scholar]
  16. Goodman E. M., Sauer H. W., Sauer L., Rusch H. P. 1969; Polyphosphate and other phosphorus compounds during growth and differentiation of Physarum polycephalum. Canadian Journal of Microbiology 15:1325–1331
    [Google Scholar]
  17. Grant B. R., Griffith J. M., Irving H. R., Radda M. 1984; An improved method for the synchronous production of zoospores from Phytophthora palmivora. Experimental Mycology 8:382–385
    [Google Scholar]
  18. Guerrini A. M., Nazzarena B., Donini P. 1980; Chromatographic separation and identification of short-chain acid-soluble polyphosphates from Saccharomyces cerevisiae. Journal of Chromatography 189:440–444
    [Google Scholar]
  19. Guest D. I. 1984; Modification of defence responses in tobacco and capsicum following treatment with fosetyl-Al. Physiological Plant Pathology 24:125–134
    [Google Scholar]
  20. Harold F. M. 1960; Accumulation of inorganic polyphosphate in mutants of Neurospora crassa. Biochimica et Biophysica Acta 45:172–188
    [Google Scholar]
  21. Harold F. M. 1963; Accumulation of inorganic polyphosphates in Aerobacter aerogenes. Journal of Bacteriology 86:216–221
    [Google Scholar]
  22. Harold F. M. 1966; Inorganic polyphosphates in biology: structure, metabolism, and function. Bacteriological Reviews 30:772–794
    [Google Scholar]
  23. Hendrix J. W., Rouser G. 1976; Polar lipids of Phytophthora parasitica var. nicotianae, in comparison with those of selected other fungi. Mycologia 68:354–361
    [Google Scholar]
  24. Jacobson L., Halmann M., Yariv J. 1982; The molecular composition of the volutin granule in yeast. Biochemical Journal 201:473–479
    [Google Scholar]
  25. Kjeldstad B., Johnsson A. 1987; A 31P-NMR study of Propionibacterium acnes, including effects caused by near-ultraviolet irradiation. Biochimica et Biophysica Acta 927:184–189
    [Google Scholar]
  26. Kulaev I. S., Vagabov V. M. 1983; Polyphosphate metabolism in microorganisms. Advances in Microbial Physiology 24:83–171
    [Google Scholar]
  27. Langen P., Liss E. 1958; Uber Bildung und Umsatz der Polyphosphate der Hefe. Biochemische Zeitschrift 330:455–466
    [Google Scholar]
  28. Langen P., Liss E., Lohman K. 1962; Art, Bildung und Umsatz der Polyphosphate der Hefe. Colloques Intemationaux du Centre National de la Recherche Scientifique 106:603–612
    [Google Scholar]
  29. Ludwig J. R. II Oliver S. G., Mclaughlin C. S. 1977; The effect of aminoacids on growth and phosphate metabolism in a prototrophic yeast strain. Biochemical and Biophysical Research Communications 79:16–23
    [Google Scholar]
  30. Navon G., Shulman R. G., Yamane T., Eccleshall T. R., Lam K.-B., Baronofsky J. J., Marmur J. 1979; Phosphorus-31 nuclear magnetic resonance studies of wild-type and glycolytic pathway mutants of Saccharomyces cerevisiae. Biochemistry 18:4487–4499
    [Google Scholar]
  31. Nicolay K., Scheffers W. A., Bruinenberg P. M., Kaptein R. 1983; In vivo 31P NMR studies on the role of the vacuole in phosphate metabolism in yeasts. Archives of Microbiology 134:270–275
    [Google Scholar]
  32. Quin L. D. 1965; The presence of compounds with a carbon-phosphorus bond in some marine invertebrates. Biochemistry 4:324–330
    [Google Scholar]
  33. Ribeiro O. K., Erwin D. C., Zentmeyer G. A. 1965; An improved synthetic medium for oospore production and germination of several Phytophthora species. Mycologia 67:1012–1019
    [Google Scholar]
  34. Rosenberg H. 1964; Distribution and fate of 2-aminoethylphos-phonic acid in Tetrahymena. Nature; London: 203299–300
    [Google Scholar]
  35. Rosenberg H. 1966; The isolation and identification of ‘volutin’ granules from Tetrahymena. Experimental Cell Research 41:397–410
    [Google Scholar]
  36. Schwinn F. J., Urech P. A. 1986; Progress in the chemical control of diseases caused by Oomycetes. In Fungicide Chemistry: Advances and Practical Applications (ACS Symposium no.304) pp. 89–106 Green M. B., Spiller D. A. Edited by Washington, DC: American Chemical Society;
    [Google Scholar]
  37. Stillway L. W., Harmon S. J. 1980; A procedure for detecting phosphonolipids on thin layer chromatograms. Journal of Lipid Research 21:1141–1143
    [Google Scholar]
  38. Torriani A., Rao N. N., Roberts M. F. 1985; Amount and chain length of polyphosphates in Escherichia coli depend on cell growth conditions. Journal of Bacteriology 162:242–247
    [Google Scholar]
  39. Wassef M. K., Hendrix J. W. 1974; Lipid content of cured tobacco. Canadian Journal of Botany 52:1123–1126
    [Google Scholar]
  40. Wassef M. K., Hendrix J. W. 1977; Ceramide aminoethylphos-phonate in the fungus Pythium prolatum. Biochimica et Biophysica Acta 486:172–178
    [Google Scholar]
  41. Wood H. G., Clark J. E. 1988; Biological aspects of inorganic polyphosphates. Annual Review of Biochemistry 57:235–260
    [Google Scholar]
  42. Wood H. G., Clark J. E., Beegen H. 1986; Isolation of intact chains of polyphosphate from ‘Propionibacterium shermanii’ grown on glucose or lactate. Journal of Bacteriology 168:1212–1219
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-1-147
Loading
/content/journal/micro/10.1099/00221287-136-1-147
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error