Transformation in Restriction-deficient LT2 Free

Abstract

Stable restriction-deficient, modification-proficient (JR501) and F′ (JR502) strains of were constructed and the effects of restriction on transformation by plasmid pBR322 were tested. Several factors which affect transformation efficiency were systematically examined to determine optimum transformation conditions and a simplified method is presented.

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1989-09-01
2024-03-29
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References

  1. Bochner B., Huang G., Schieven G.L., Ames B.N. 1980; Positive selection for loss of tetracycline resistance. Journal of Bacteriology 143:926–933
    [Google Scholar]
  2. Bullas L.R., Colson C. 1975; DNA restriction and modification systems in Salmonella. III. SP, a Salmonella potsdam system allelic to the SB system in Salmonella typhimurium . Molecular and General Genetics 139:177–188
    [Google Scholar]
  3. Bullas L.R., Ryu J. 1983; Salmonella typhimurium LT2 strains which are rm+ for all three chromosomally located systems of DNA restriction and modification. Journal of Bacteriology 156:471–474
    [Google Scholar]
  4. Bullas L.R., Colson C., Neufeld B. 1980; Deoxyribonucleic acid restriction and modification systems in Salmonella: chromosomally located systems of different serotypes. Journal of Bacteriology 141:275–292
    [Google Scholar]
  5. Bursztyn H., Sgaramella V., Cifferri O., Lederberg J. 1975; Transfectability of rough strains of Salmonella typhimurium . Journal of Bacteriology 124:1630–1634
    [Google Scholar]
  6. Cohen S.N., Chang A.C.Y., Hsu L. 1972; Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proceedings of the National Academy of Sciences of the United States of America 69:2110–2114
    [Google Scholar]
  7. Colson C., Van Pel A. 1974; DNA restriction and modification systems in Salmonella. I. SA and SB, two Salmonella typhimurium systems determined by genes with a chromosomal location comparable to that of the Escherichia coli hsd genes. Molecular and General Genetics 129:325–337
    [Google Scholar]
  8. Dagert M., Ehrlich S.D. 1979; Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene 6:23–28
    [Google Scholar]
  9. Davis L.G., Dibner M.D., Battey J.F. 1986 Basic Methods in Molecular Biology pp. 89–104 New York:: Elsevier.;
    [Google Scholar]
  10. Davis R.W., Botstein D., Roth J.R. 1980 Advanced Bacterial Genetics. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  11. Haima P., Bron S., Venema G. 1987; The effect of restriction on shotgun cloning and plasmid stability in Bacillus subtilis Marburg. Molecular and General Genetics 209:335–342
    [Google Scholar]
  12. Hanahan D. 1983; Studies of transformation of Escherichia coli with plasmids. Journal of Molecular Biology 166:557–580
    [Google Scholar]
  13. Hanahan D. 1987; Mechanism of DNA transformation. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology 2 pp. 1177–1183 Neidhardt C. F. others Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  14. Kozdroj H., Klopotowski T. 1979; The smoB mutation suppressing cell filamentation and ability to support the multiplication of phage P22 in Salmonella typhimurium . Acta biochemica polonica 26:135–143
    [Google Scholar]
  15. Kushner S.R. 1978; An improved method for transformation of Escherichia coli with ColEl derived plasmid. In Proceedings of International Symposium on Genetic Engineering pp. 17–23 Boyer H. W., Ricosia S. Edited by Amsterdam: Elsevier/ North Holland Biomedical Press;
    [Google Scholar]
  16. Lederberg E.M., Cohen S.N. 1974; Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid. Journal of Bacteriology 119:1072–1074
    [Google Scholar]
  17. Maclachlan P.R., Sanderson K.E. 1985; Transformation of Salmonella typhimurium with plasmid DNA: differences between rough and smooth strains. Journal of Bacteriology 161:442–445
    [Google Scholar]
  18. Mandel M., Higa A. 1970; Calcium-dependent bacteriophage DNA infection. Journal of Molecular Biology 53:159–162
    [Google Scholar]
  19. Maniatis T., Fritsch E.F., Sambrook J. 1982 In Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  20. Matsushima P., Cox K.L., Balts R.H. 1987; Highly transformable mutants of Streptomyces fra-diae defective in several restriction systems. Molecular and General Genetics 206:393–400
    [Google Scholar]
  21. Merrick M.J., Gibbins J.R., Postgate J.R. 1987; A rapid and efficient method for plasmid transformation of Klebsiella pneumoniae and Escherichia coli . Journal of General Microbiology 133:2053–2057
    [Google Scholar]
  22. Mizuno T., Mutoh N., Panasenko S.M., Imae Y. 1986; Acquisition of maltose chemotaxis in Salmonella typhimurium by the introduction of the Escherichia coli chemosensory transducer gene. Journal of Bacteriology 165:890–895
    [Google Scholar]
  23. Nagaraja V., Shepherd J.C.W., Pripfl T., Bickle T.A. 1985; Two type I restriction enzymes from Salmonella species: purification and DNA recognition sequences. Journal of Molecular Biology 182:579–587
    [Google Scholar]
  24. Norgard M.V., Keem K., Monahan J.J. 1978; Factors affecting the transformation of Escherichia coli strain X1776 by pBR322 plasmid DNA. Gene 3:279–292
    [Google Scholar]
  25. Oishi M., Irbe R.M. 1977; Circular chromosomes and genetic transformation in Escherichia coli . In Modern Trends in Bacterial Transformation and Transfection pp. 121–134 Portoles A., Lopez R., Espinosa M. Edited by Amsterdam: North-Holland Publishing Co;
    [Google Scholar]
  26. Sanderson K.E., Hurley J.A. 1987; Linkage map of Salmonella typhimurium . In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology 2 pp. 877–918 Neidhardt F. C. others Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  27. Sanderson K.E., Stocker B.A.D. 1987; Salmonella typhimurium strains used in genetic analysis. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology 2 pp. 1220–1224 Neidhardt F. C. others Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  28. Saunders V.A., Saunders J.R. 1987 In Microbial Genetics Applied to Biotechnology. Principles and Techniques of Gene Transfer and Manipulation pp. 3–20 New York:: Macmillan Publishing Company.:
    [Google Scholar]
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