1887

Abstract

A gene coding for endo-l,4--glucanase activity has been isolated from subsp. by cloning in . After restriction mapping of a 6·4 kb insert, a 2·2 kb DNA fragment was sub-cloned in pUC19 to produce the enzymically active clone pJW3. Recloning of the gene fragment in the reverse orientation in pUC18 (clone pJW4) indicated that a gene promoter was present in the cloned fragment and was able to function in . The clone pJW4 displayed increased activity which was attributed to expression from the promoter of pUC18. The enzyme encoded by pJW4 was optimally active at pH 5·5–6·0, and in the temperature range 37–42 °C. The preferred substrate was carboxymethylcellulose, but the enzyme displayed 50–60% of maximal activity on both acid-swollen cellulose and soluble xylan. No significant activity was detected on ball-milled filter paper or particulate xylan. Deletion experiments confirmed that both cellulase and xylanase activities were altered to a similar extent by deletion of DNA from the 3′ end of the gene, suggesting that both are a function of the same polypeptide product.

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1989-09-01
2021-07-31
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