1887

Abstract

At elevated temperatures, yeast cells of . synthesized nine heat-shock proteins (HSPs) with apparent molecular masses of 98, 85, 81, 76, 72, 54, 34, 26 and 18 kDa. The optimum temperature for the heat-shock response was 45C although HSPs were detected throughout the range 41–46 °C. Protein synthesis was not observed in cells kept at 48 °C. Yeast cells survived exposure to an otherwise lethal temperature of 55C when they had previously been exposed to 45 °C. The thermotolerance induced during incubation at 45 °C required protein synthesis, since protection was markedly reduced by trichodermin. Mercury ions induced a set of three stress proteins, one of which corresponded in size to an HSP, and cadmium ions evoked one stress protein seemingly unrelated to the HSPs observed after temperature shift.

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1989-09-01
2021-05-17
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