1887

Abstract

At elevated temperatures, yeast cells of . synthesized nine heat-shock proteins (HSPs) with apparent molecular masses of 98, 85, 81, 76, 72, 54, 34, 26 and 18 kDa. The optimum temperature for the heat-shock response was 45C although HSPs were detected throughout the range 41–46 °C. Protein synthesis was not observed in cells kept at 48 °C. Yeast cells survived exposure to an otherwise lethal temperature of 55C when they had previously been exposed to 45 °C. The thermotolerance induced during incubation at 45 °C required protein synthesis, since protection was markedly reduced by trichodermin. Mercury ions induced a set of three stress proteins, one of which corresponded in size to an HSP, and cadmium ions evoked one stress protein seemingly unrelated to the HSPs observed after temperature shift.

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/content/journal/micro/10.1099/00221287-135-9-2509
1989-09-01
2023-12-01
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References

  1. Ahrens J.C., Daneo-Moore L., Buckley H.R. 1983; Differential protein synthesis in Candida albicans during blastospore formation at 24·5 °C and during germ tube formation at 37 °C. Journal of General Microbiology 129:1133–1139
    [Google Scholar]
  2. Ashburner M., Bonner J.J. 1979; The induction of gene activity in Drosophila by heat shock. Cell 17:241–254
    [Google Scholar]
  3. Atkinson B.G., Dean R.L. 1985; Effect of stress on the gene expression of amphibian, avian, and mammalian blood cells. In Changes in Eukaryotic Gene Expression in Response to Environmental Stress pp. 159–181 Atkinson B.G., Walden D.B. Edited by New York: Academic Press;
    [Google Scholar]
  4. Borg M., Ruchel R. 1988; Expression of extracellular proteinase by proteolytic Candida spp. during experimental infection of oral mucosa. Infection and Immunity 56:626–631
    [Google Scholar]
  5. Brown L.A., Chaffin W.L. 1981; Differential expression of cytoplasmic proteins during yeast bud and germ tube formation in Candida albicans . Canadian Journal of Microbiology 27:580–586
    [Google Scholar]
  6. Brummel M., Soll D.R. 1982; The temporal regulation of protein synthesis during synchronous bud or mycelium formation in the dimorphic yeast Candida albicans . Developmental Biology 89:211–224
    [Google Scholar]
  7. Carper S.W., Duffy J.J., Gerner E.W. 1987; Heat shock proteins in thermotolerance and other cellular processes. Cancer Research 47:5249–5255
    [Google Scholar]
  8. Dabrowa N., Howard D.H. 1983; Blastoconi-dium germination. In Fungi Pathogenic for Humans and Animals, Part A, Biology pp. 525–545 Howard D.H. Edited by New York: Marcel Dekker;
    [Google Scholar]
  9. Dabrowa N., Howard D.H. 1984; Heat shock and heat stroke proteins observed during germination of the blastoconidia of Candida albicans . Infection and Immunity 44:537–539
    [Google Scholar]
  10. Dabrowa N., Howard D.H. 1988; Nutritionally induced changes in protein synthesis of Candida albicans . Abstracts of the Annual Meeting of the American Society for Microbiology F2:392
    [Google Scholar]
  11. Dabrowa N., Howard D.H., Landau J.N., Schechter Y. 1970; Synthesis of nucleic acids and proteins in the dimorphic forms of Candida albicans . Sabouraudia 8:163–169
    [Google Scholar]
  12. Gibbons G.F., Howard D.H. 1986; Arginine auxotrophs of Candida albicans deficient in argino-succinate lyase. Journal of General Microbiology 132:263–268
    [Google Scholar]
  13. Gopal P., Sullivan P.A., Shepherd M.G. 1982; Enzymes of iV-acetylglucosamine metabolism during germ-tube formation in Candida albi-cans . Journal of General Microbiology 128:2319–2326
    [Google Scholar]
  14. Gottesman S. 1984; Bacterial regulation: global regulatory networks. Annual Review of Genetics 18:415–441
    [Google Scholar]
  15. Guy C.L., Plesofsky-Vig N., Brambl R. 1986; Heat shock protects germinating conidiospores of Neurospora crassa against freezing injury. Journal of Bacteriology 167:124–129
    [Google Scholar]
  16. Hedstrom R., Culpepper J., Harrison R.A., Agabian N., Newport G. 1987; A major immunogen in Schistosoma mansoni infections is homologous to the heat-shock protein Hsp 70. Journal of Experimental Medicine 165:1430–1435
    [Google Scholar]
  17. Howard D.H., Zeuthen M.L., Dabrowa N. 1986; Phenotypic characteristics of a slow-growing, nongerminative variant of Candida albicans . Journal of General Microbiology 132:2359–2366
    [Google Scholar]
  18. Ingolia T.D., Slater M.R., Craig E.A. 1982; Saccharomyces cerevisiae contains a complex multigene family related to the major heat-inducible gene of Drosophila . Molecular and Cellular Biology 2:1388–1398
    [Google Scholar]
  19. Jenkins D.E., Schultz J.E., Matin A. 1988; Starvation-induced cross protection against heat or H202 challenge in Escherichia coli . Journal of Bacteriology 170:3910–3914
    [Google Scholar]
  20. Kapoor M., Lewis J. 1987; Alteration of the protein synthesis pattern in Neurospora crassa cells by hyperthermal and oxidative stress. Canadian Journal of Microbiology 33:162–168
    [Google Scholar]
  21. Kelly P.M., Schlesinger M.J. 1982; Antibodies to two major heat-shock proteins cross-react with similar proteins in widely divergent species. Molecular and Cellular Biology 2:267–274
    [Google Scholar]
  22. Lambowitz A.M., Kobayashi G.S., Painter A., Medoff G. 1983; Morphogenesis in the pathogenic fungus Histoplasma capsulatum may be related to heat shock response. Nature; London: 303806–808
    [Google Scholar]
  23. Lee K.L., Buckley H.R., Campbell C.C. 1975; An amino acid liquid synthetic medium for the development of mycelial and yeast forms of Candida albicans . Sabouraudia 13:148–153
    [Google Scholar]
  24. Lejohn H.B., Braithwaite C.E. 1984; Heat and nutritional shock-induced proteins of the fungus Achlya are different and under independent tran-scriptional control. Canadian Journal of Biochemistry and Cell Biology 62:837–846
    [Google Scholar]
  25. Li G.C., Laszlo A. 1985; Thermotolerance in mammalian cells: a possible role for heat shock proteins. In Changes in Eukaryotic Gene Expression in Response to Environmental Stress pp. 227–254 Atkinson B.G., Walden D.B. Edited by New York: Academic Press;
    [Google Scholar]
  26. Lindquist S. 1986; The heat-shock response. Annual Review of Biochemistry 55:1151–1191
    [Google Scholar]
  27. Manning M., Mitchell T.G. 1980; Morphogenesis of Candida albicans and cytoplasmic proteins associated with differences in morphology, strain, or temperature. Journal of Bacteriology 144:258–273
    [Google Scholar]
  28. Morrisey J.H. 1981; Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Analytical Biochemistry 117:307–310
    [Google Scholar]
  29. Odds C. 1988 Candida and Candidosis, 2nd edn.. London: Bailliere Tindall;
    [Google Scholar]
  30. Plesofsky-VIG N., Brambl R. 1985; Heat shock response in Neurospora crassa: protein synthesis and induced thermotolerance. Journal of Bacteriology 162:1083–1091
    [Google Scholar]
  31. Plesset J., Palm C., Mclaughlin C.S. 1982; Induction of heat shock proteins and tolerance by ethanol in Saccharomyces cerevisiae . Biochemical and Biophysical Research Communications 10 8:1340–1345
    [Google Scholar]
  32. Ray T.L. 1988; Scanning electron microscopy of epidermal adherence and cavitation in murine candidiasis: a role for Candida acid proteinase. Infection and Immunity 56:1942–1949
    [Google Scholar]
  33. Schlesinger M.J. 1985; Stress response in avian cells. In Changes in Eukaryotic Gene Expression in Response to Environmental Stress pp. 183–195 Atkinson B.G., Walden D.B. Edited by New York: Academic Press;
    [Google Scholar]
  34. Schlesinger M.J. 1986; Heat shock proteins: the search for functions. Journal of Cell Biology 103:321–325
    [Google Scholar]
  35. Shepherd M.G., Yin CY.Y., Ram S.P., Sullivan P.A. 1980; Germ tube induction in Candida albicans . Canadian Journal of Microbiology 26:21–26
    [Google Scholar]
  36. Syverson R.E., Buckley H.R., Campbell C.C. 1975; Cytoplasmic antigens unique to the mycelial or yeast phase of Candida albicans . Infection and Immunity 12:1183–1188
    [Google Scholar]
  37. Wanner R., Forster H., Mendgen K., Staples R.C. 1985; Synthesis of differentiation-specific proteins in germlings of the wheat stem rust fungus after heat shock. Experimental Mycology 9:279–283
    [Google Scholar]
  38. Welch W.J., Feramisco J.R. 1984; Nuclear and nucleolar localization of the 72000-dalton heat shock protein in heat-shocked mammalian cells. Journal of Biological Chemistry 259:4501–4513
    [Google Scholar]
  39. Zeuthen M.L., Dabrowa N., Aniebo C.M., Howard D.H. 1988; Ethanol tolerance and the induction of stress proteins by ethanol in Candida albicans . Journal of General Microbiology 134:1375–1384
    [Google Scholar]
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