@article{mbs:/content/journal/micro/10.1099/00221287-135-9-2503, author = "Moldoveanu, Natalia and Kates, Morris", title = "Effect of Bacitracin on Growth and Phospholipid, Glycolipid and Bacterioruberin Biosynthesis in Halobacterium cutirubrum", journal= "Microbiology", year = "1989", volume = "135", number = "9", pages = "2503-2508", doi = "https://doi.org/10.1099/00221287-135-9-2503", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-135-9-2503", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = " Halobacterium cutirubrum was cultured in a complex medium designed for extreme halophiles containing 4 m-NaCl in the absence or presence of bacitracin (7, 14 or 28 μm) with shaking at 37 °C for 24 h. Bacitracin at 7 or 14 μm inhibited growth (50% and 100%, respectively), phospholipid synthesis (88% and 96%, respectively) and bacterioruberin synthesis (65% and 87%, respectively) relative to a control lacking bacitracin. After 5 h incubation in the presence of 7 or 28 μm-bacitracin, total lipid biosynthesis, as measured by incorporation of 14C from short-term labelling with [14C]glycerol, was inhibited by 26% and 66%, respectively. Bacitracin at 7 or 28μm inhibited biosynthesis of the major phospholipids (9% and 60%), glycolipids (25% and 85%) and polyisoprenyl pyrophosphate (75% at both concentrations). These results support the concept that polyisoprenyl pyrophosphate is involved directly in the biosynthesis of phospholipids, glycolipids and bacterioruberins in the extreme halophile H. cutirubrum. ", }