1887

Abstract

was cultured in a complex medium designed for extreme halophiles containing 4 -NaCl in the absence or presence of bacitracin (7, 14 or 28 μ) with shaking at 37 °C for 24 h. Bacitracin at 7 or 14 μ inhibited growth (50% and 100%, respectively), phospholipid synthesis (88% and 96%, respectively) and bacterioruberin synthesis (65% and 87%, respectively) relative to a control lacking bacitracin. After 5 h incubation in the presence of 7 or 28 μ-bacitracin, total lipid biosynthesis, as measured by incorporation of C from short-term labelling with [C]glycerol, was inhibited by 26% and 66%, respectively. Bacitracin at 7 or 28μ inhibited biosynthesis of the major phospholipids (9% and 60%), glycolipids (25% and 85%) and polyisoprenyl pyrophosphate (75% at both concentrations). These results support the concept that polyisoprenyl pyrophosphate is involved directly in the biosynthesis of phospholipids, glycolipids and bacterioruberins in the extreme halophile .

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/content/journal/micro/10.1099/00221287-135-9-2503
1989-09-01
2021-07-25
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