1887

Abstract

The levels of several enzymes involved in assimilation of different nitrogen compounds were investigated in in relation to the nitrogen source supplied to the cultures. Threonine dehydratase, serine dehydratase, proline dehydrogenase, histidase and urocanase were not decreased in the presence of ammonium. The latter two enzymes were induced by histidine in the culture medium, while proline dehydrogenase was induced by proline. Glutamine synthetase, urease and ornithine aminotransferase levels were higher with poor nitrogen sources and were repressed by ammonium. Arginase was induced by arginine and repressed by ammonium. Glutamine synthetase was rapidly inactivated upon addition of ammonium to the culture, and could be reactivated by treatment with snake venom phosphodiesterase, which suggested that adenylylation is involved in the inactivation. Three previously isolated mutants with abnormal glutamine synthetase activities showed pleiotropic effects on urease formation. All these data point to a mechanism controlling preferential utilization of some nitrogen sources in this species.

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1989-09-01
2022-01-18
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References

  1. Aharonowitz Y., Demain A.L. 1977; Influence of inorganic phosphate and organic buffers on cephalosporin production by Streptomyces clavuligerus . Archives of Microbiology 115:169–173
    [Google Scholar]
  2. BascarÁn V., Hardisson C., Braña A.F. 1989; Isolation and characterization of nitrogen-deregulated mutants of Streptomyces clavuligerus . Journal of General Microbiology 135:2475–2482
    [Google Scholar]
  3. Baumberg S., Harwood C.R. 1979; Carbon and nitrogen repression of arginine catabolic enzymes in Bacillus subtilis . Journal of Bacteriology 137:189–196
    [Google Scholar]
  4. Braña A.F., Demain A.L. 1988; Nitrogen control of antibiotic biosynthesis in actinomycetes. In Nitrogen Source Control of Microbial Processes pp. 99–119 Sánchez-Esquivel S. . Edited by Boca Ratón, Fla: CRC Press;
    [Google Scholar]
  5. Braña A.F., Wolfe S., Demain A.L. 1985; Ammonium repression of cephalosporin production by Streptomyces clavuligerus . Canadian Journal of Microbiology 31:736–743
    [Google Scholar]
  6. Braña A.F., Paiva N., Demain A.L. 1986; Pathways and regulation of ammonium assimilation in Streptomyces clavuligerus . Journal of General Microbiology 132:1305–1317
    [Google Scholar]
  7. Broman K., Lauwers N., Stalon V., Wiame J.M. 1978; Oxygen and nitrate in utilization by Bacillus licheniformis of the arginase and arginine deiminase routes of arginine catabolism and other factors affecting their synthesis. Journal of Bacteriology 135:920–927
    [Google Scholar]
  8. Castro J.M., Liras P., Cortes J., Martin J.F. 1985; Regulation of a-aminoadiphyl-cysteinyl-valine, isopenicillin N synthetase, isopenicillin N isomerase and deacetoxycephalosporin C synthetase by nitrogen sources in Streptomyces lactamdurans . Applied Microbiology and Biotechnology 21:32–40
    [Google Scholar]
  9. Dendinger S., Brill W.J. 1970; Regulation of proline degradation in Salmonella typhimurium . Journal of Bacteriology 103:144–152
    [Google Scholar]
  10. Dyson P., Schrempf H. 1987; Genetic instability and DNA amplification in Streptomyces lividans 66. Journal of Bacteriology 169:4796–4803
    [Google Scholar]
  11. Fornwald J.A., Schmidt F.J., Adams C.W., Rosenberg M., Brawner M.E. 1987; Two promoters, one inducible and one constitutive, control transcription of the Streptomyces lividansgalactose operon. Proceedings of the National Academy of Sciences of the United States of America 84:2130–2134
    [Google Scholar]
  12. Friedrich B., Magasanik B. 1977; Urease of Klebsiella aerogenes: control of its synthesis by glutamine synthetase. Journal of Bacteriology 131:446–452
    [Google Scholar]
  13. Friedrich B., Friedrich C.G., Magasanik B. 1978; Catabolic N2 -acetylornithine 5-aminotransferase of Klebsiella aerogenes:control of synthesis by induction, catabolite repression, and activation by glutamine synthetase. Journal of Bacteriology 133:686–691
    [Google Scholar]
  14. Gottesman S. 1984; Bacterial regulation: global regulatory networks. Annual Review of Genetics 18:415–441
    [Google Scholar]
  15. GrÄfe U., Bocker H., Thrum H. 1979; Regulation der Glutamin-synthetasebildung in Streptomyceten. Zeitschrift für allgemeine Mikrobiologie 19:663–666
    [Google Scholar]
  16. Hill D.W., Walters F.H., Wilson D., Stuart J.D. 1979; High performance liquid chromatographic determination of amino acids in the pico-mole range. Analytical Chemistry 51:1338–1341
    [Google Scholar]
  17. Hodgson D.A. 1982; Glucose repression of carbon source uptake and metabolism in Streptomyces coelicolorA3(2) and its perturbation in mutants resistant to 2-deoxyglucose. Journal of General Microbiology 128:2417–2430
    [Google Scholar]
  18. Ikeda H., Seno E.T., Bruton C.J., Chater K.F. 1984; Genetic mapping, cloning and physiological aspects of the glucose kinase gene of Streptomyces coelicolor . Molecular and General Genetics 196:501–507
    [Google Scholar]
  19. Janssen D.B., Herst P.M., Joosten H.M.L.J., Van Der Drift C. 1981; Nitrogen control in Pseudomonas aeruginosa: a role for glutamine in the regulation of the synthesis of NADP-dependent glutamate dehydrogenase, urease and histidase. Archives of Microbiology 128:398–402
    [Google Scholar]
  20. Kaltwasser H., Kramer J., Conger W.R. 1972; Control of urease formation in certain aerobic bacteria. Archives of Microbiology 81:178–196
    [Google Scholar]
  21. Kendrick K.E., Wheelis M.L. 1982; Histidine dissimilation in Streptomyces coelicolor . Journal of General Microbiology 128:2029–2040
    [Google Scholar]
  22. Kominek L.A. 1972; Biosynthesis of novobiocin by Streptomyces niveus . Antimicrobial Agents and Chemotherapy 1:123–134
    [Google Scholar]
  23. Kroening T.A., Kendrick K.E. 1987; In vivo regulation of histidine ammonia-lyase activity from Streptomyces griseus . Journal of Bacteriology 169:823–829
    [Google Scholar]
  24. Magasanik B. 1982; Genetic control of nitrogen assimilation in bacteria. Annual Review of Genetics 16:135–168
    [Google Scholar]
  25. Martin J.F., Demain A.L. 1980; Control of antibiotic biosynthesis. Microbiological Reviews 44:230–251
    [Google Scholar]
  26. MartÍn M.C., Diaz L.A., Manzanal M.B., Hardisson C. 1986; Role of trehalose in the spores of Streptomyces . FEMS Microbiology Letters 35:49–54
    [Google Scholar]
  27. Meile L., Soldati L., Leisinger T. 1982; Regulation of proline catabolism in Pseudomonas aeruginosa PAO. Archives of Microbiology 132:189–193
    [Google Scholar]
  28. Merrick M.J. 1988; Regulation of nitrogen assimilation by bacteria. In The Nitrogen and Sulphur Cycles pp. 331–361 Cole J. A., Ferguson S. J. Edited by Cambridge: Cambridge University Press;
    [Google Scholar]
  29. Newman E.B., Kapoor V., Potter R. 1976; Role of l-threonine dehydrogenase in the catabolism of threonine and synthesis of glycine by Escherichia coli . Journal of Bacteriology 126:1245–1249
    [Google Scholar]
  30. Omura S., Tanaka Y. 1985; Biosynthesis of tylosin and its regulation by ammonium and phosphate. In Regulation of Secondary Metabolite Formation pp. 305–332 Kleinkauf H., Von Döhren H., Dornauer H., Nesemann G. Edited by Weinheim, FRG: VCH Verlagsgesellschaft;
    [Google Scholar]
  31. Pahel G., Zelenetz A.D., Tyler B. 1978; gltBgene and regulation of nitrogen metabolism by glutamine synthetase in Escherichia coli . Journal of Bacteriology 133:139–148
    [Google Scholar]
  32. Pardee A.B., Prestidge L.S. 1955; Induced formation of serine and threonine deaminases by Escherichia coli . Journal of Bacteriology 70:667–674
    [Google Scholar]
  33. Paress P.S., Streicher S.L. 1985; Glutamine synthetase of Streptomyces cattleya: purification and regulation of synthesis. Journal of General Microbiology 131:1903–1910
    [Google Scholar]
  34. Patterson J.A., Hespell R.B. 1985; Glutamine synthetase activity in the ruminal bacterium Succini-vibrio dextrinosolvens . Applied and Environmental Microbiology 50:1014–1020
    [Google Scholar]
  35. Romero J., Liras P., Martin J.F. 1986; Utilization of ornithine and arginine as specific precursors of clavulanic acid. Applied and Environmental Microbiology 52:892–897
    [Google Scholar]
  36. Seno E.T., Chater K.F. 1983; Glycerol catabolic enzymes and their regulation in wild-type and mutant strains of Streptomyces coelicolorA3(2). Journal of General Microbiology 129:1403–1413
    [Google Scholar]
  37. Shapiro S., Vining L.C. 1983; Nitrogen metabolism and chloramphenicol production in Streptomyces venezuelae . Canadian Journal of Microbiology 29:1706–1714
    [Google Scholar]
  38. Shapiro S., Vining L.C. 1984; Suppression of nitrate utilization by ammonium and its relationship to chloramphenicol production in Streptomyces venezuelae . Canadian Journal of Microbiology 30:798–804
    [Google Scholar]
  39. Shapiro S., Vining L.C. 1985; Effect of ammonium on chloramphenicol production by Streptomyces venezuelae . Canadian Journal of Microbiology 31:119–123
    [Google Scholar]
  40. Smith C.J., Hespell R.B., Bryant M.P. 1981; Regulation of urease and ammonia assimilatory enzymes in Selenomonas ruminantium . Applied and Environmental Microbiology 42:89–96
    [Google Scholar]
  41. Streicher S.L., Tyler B. 1981; Regulation of glutamine synthetase activity by adenylylation in the Gram-positive bacterium Streptomyces cattleya . Proceedings of the National Academy of Sciences of the United States of America 78:229–233
    [Google Scholar]
  42. Tyler B. 1978; Regulation of the assimilation of nitrogen compounds. Annual Review of Biochemistry 47:1127–1162
    [Google Scholar]
  43. VancurÁ A., Rezanka T., Marslalek J., Melzoch K., Basarova G., Kristan V. 1988; Metabolism of l-threonine and fatty acids and tylosin biosynthesis in Streptomyces fradiae . FEMS Microbiology tetters 49:411–415
    [Google Scholar]
  44. Vancurová I., Vancura A., Volc J., Neuzil J., Flieger M., Basarova G., Behal V. 1988; Isolation and characterization of valine dehydrogenase from Streptomyces aureofaciens . Journal of Bacteriology 170:5192–5196
    [Google Scholar]
  45. Virolle M.J., Bibb M.J. 1988; Cloning, characterization and regulation of an α-amylase gene from Streptomyces limosus . Molecular Microbiology 2:197–208
    [Google Scholar]
  46. Weatherburn M.W. 1967; Phenol-hypochlorite reaction for determination of ammonia. Analytical Chemistry 39:971–974
    [Google Scholar]
  47. Wray L.V., Fisher S.H. 1988; Nitrogen regulation in Streptomyces coelicolor . 7th International Symposium on Biology of ActinomycetesMay 1988 Tokyo, Japan: abstract Sla-3.
    [Google Scholar]
  48. Xia T., Jiao R.S. 1986; Studies on glutamine synthetase from Streptomyces hygroscopicus var. jinggangensis . Scientia sinica(Series B) 29:379–388
    [Google Scholar]
  49. Zimmermann F.K., Eaton N.R. 1974; Genetics of induction and catabolite repression of maltase synthesis in Saccharomyces cerevisiae . Molecular and General Genetics 134:261–272
    [Google Scholar]
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