1887

Abstract

Fourteen lipase low-producing (Lip) mutants of were obtained by UV mutagenesis. Complementation studies showed that ten of these mutants belonged to one complementation group. Two other mutants, Lip 132 and 223, complemented those of the first group. Lip 1123 complemented mutants of the first and second groups. Lip 1351 did not complement Lip 742, 417, 529 and 1133, which belonged to the first group. Genetic recombination analysis of the Lip mutants by random ascospore analysis showed that mutants of the first and second groups have lesions in the same gene, which is proposed to be the lipase structural gene. Inter-allelic complementation occurs between certain mutants of these groups. Lip 1123 has a lesion in a different gene.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-9-2439
1989-09-01
2024-12-13
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/9/mic-135-9-2439.html?itemId=/content/journal/micro/10.1099/00221287-135-9-2439&mimeType=html&fmt=ahah

References

  1. Barth G., Weber H. 1985; Improvement of sporulation in the yeast Yarrowia lipolytica . Antonie van Leeuwenhoek 51:167–177
    [Google Scholar]
  2. Beckerich J.M., Ceccaldi B.C., Lambert M., Heslot H. 1984; Evidence for the control of a mutation in lysine catabolism by the mating type in Yarrowia lipolytica . Current Genetics 8:531–536
    [Google Scholar]
  3. Davidow L.S., Apostolakos D., O’Donnell M.M., Proctor A.R., Ogrydziak D., Wing R.A., Stasko I., De Zeeuw J.R. 1985; Integrative transformation of the yeast Yarrowia lipolytica . Current Genetics 10:39–48
    [Google Scholar]
  4. Davidow L.S., O’Donnell M.D., Kacmarek F.S., Pereira D.A., De Zeeuw J.R., Franke A.E. 1987; Cloning and sequencing of the alkaline extracellular protease gene of Yarrowia lipolytica . Journal of Bacteriology 169:4621–4629
    [Google Scholar]
  5. Gaillardin C.M., Charoy V., Heslot H. 1973; A study of copulation, sporulation and meiotic segregation in Candida lipolytica . Archives of Microbiology 92:69–83
    [Google Scholar]
  6. Gaillardin C.M., Ribet A.M., Heslot H. 1985; Integrative transformation of the yeast Yarrowia lipolytica . Current Genetics 10:49–58
    [Google Scholar]
  7. Ogrydziak D.M., Scharf S.J. 1982; Alkaline extracellular protease produced by Saccharomycopsis lipolytica CX 161-IB. Journal of General Microbiology 128:1225–1234
    [Google Scholar]
  8. Ogrydziak D.M., Bassel J., Mortimer R. 1982; Development of the genetic map of the yeast Yarrowia lipolytica . Molecular and General Genetics 188:179–183
    [Google Scholar]
  9. Ota Y., Yamada K. 1967; Lipase from Candida paralipolytica. Part III. Further studies on the activation of the enzyme. Agricultural and Biological Chemistry 31:809–816
    [Google Scholar]
  10. Ota Y., Yoshioka K., Minoda Y., Yamada K. 1973; Demonstration of lipid lipase activators produced by Candida paralipolytica . Agricultural and Biological Chemistry 37:2879–2883
    [Google Scholar]
  11. Ota Y., Morimoto Y., Sugiura T., Minoda Y. 1978; Soybean fraction increasing the extracellular lipase production by Saccharomycopsis lipolytica (Candida paralipolytica) . Agricultural and Biological Chemistry 42:1937–1938
    [Google Scholar]
  12. Ota Y., Gomi K., Kato S., Sugijra T., Minoda Y. 1982; Purification and some properties of cell-bound lipase from Saccharomycopsis lipolytica . Agricultural and Biological Chemistry 46:2885–2893
    [Google Scholar]
  13. PALM OIL RESEARCH INSTITUTE OF MALAYSIA 1986; Data Sheet I.Palm Oil.
    [Google Scholar]
  14. Snow R. 1966; An enrichment method for auxotrophic yeast mutants using the antibiotic ‘nystatin’. Nature, London 211:206–207
    [Google Scholar]
  15. Sugiura T., Ota Y., Yamada K. 1976; Partial characterisation of cell-bound lipase of Candida paralipolytica . Agricultural and Biological Chemistry 40:2479–2480
    [Google Scholar]
/content/journal/micro/10.1099/00221287-135-9-2439
Loading
/content/journal/micro/10.1099/00221287-135-9-2439
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error