SUMMARY: Fourteen lipase low-producing (Lip) mutants of were obtained by UV mutagenesis. Complementation studies showed that ten of these mutants belonged to one complementation group. Two other mutants, Lip 132 and 223, complemented those of the first group. Lip 1123 complemented mutants of the first and second groups. Lip 1351 did not complement Lip 742, 417, 529 and 1133, which belonged to the first group. Genetic recombination analysis of the Lip mutants by random ascospore analysis showed that mutants of the first and second groups have lesions in the same gene, which is proposed to be the lipase structural gene. Inter-allelic complementation occurs between certain mutants of these groups. Lip 1123 has a lesion in a different gene.


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