Polymerase Chain Reaction for the Detection of Free

Abstract

A polymerase chain reaction (PCR) using heat-stable polymerase is described for the specific detection of , the causative agent of leprosy. A set of primers was selected on the basis of the nucleotide sequence of a gene encoding the 36 kDa antigen of With this set of primers in the PCR, could be detected specifically with a detection limit approximating one bacterium. This PCR appears to meet the criteria of specificity and sensitivity required for a useful tool in epidemiology and eventually for the control of leprosy.

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1989-09-01
2024-03-29
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