1887

Abstract

SUMMARY: A polymerase chain reaction (PCR) using heat-stable polymerase is described for the specific detection of , the causative agent of leprosy. A set of primers was selected on the basis of the nucleotide sequence of a gene encoding the 36 kDa antigen of With this set of primers in the PCR, could be detected specifically with a detection limit approximating one bacterium. This PCR appears to meet the criteria of specificity and sensitivity required for a useful tool in epidemiology and eventually for the control of leprosy.

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/content/journal/micro/10.1099/00221287-135-9-2357
1989-09-01
2019-11-12
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-9-2357
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