1887

Abstract

A protoplast transformation system has been developed for MC14. Optimum regeneration conditions were achieved by raising the incubation temperature of the regeneration plates to 46.C. Regenerated transformed colonies could be isolated in 3 to 5 d under these conditions. Plasmids introduced by this method were stably maintained by MC14 and could be recovered and used to transform

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1989-08-01
2021-10-19
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