1887

Abstract

A protoplast transformation system has been developed for MC14. Optimum regeneration conditions were achieved by raising the incubation temperature of the regeneration plates to 46.C. Regenerated transformed colonies could be isolated in 3 to 5 d under these conditions. Plasmids introduced by this method were stably maintained by MC14 and could be recovered and used to transform

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-8-2283
1989-08-01
2024-10-10
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/8/mic-135-8-2283.html?itemId=/content/journal/micro/10.1099/00221287-135-8-2283&mimeType=html&fmt=ahah

References

  1. Barany F., Boeke J.D., Tomasz A. 1982; Staphylococcal plasmids that replicate and express erythromycin resistance in both Streptococcus pneumoniae and Escherichia coli.. Proceedings of the National Academy of Sciences of the United States of America 79:2991–2995
    [Google Scholar]
  2. Bernhard K., Schrempf H., Goebel W. 1978; Bacteriocin and antibiotic resistance plasmids in Bacillus cereus and Bacillus subtilis.. Journal of Bacteriology 133:897–903
    [Google Scholar]
  3. Bourne N., Dancer B.N. 1986; Regeneration of protoplasts of Bacillus subtilis 168 and closely related strains.. Journal of General Microbiology 132:251–255
    [Google Scholar]
  4. Chang S., Cohen S.N. 1979; High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA.. Molecular and General Genetics 168:111–115
    [Google Scholar]
  5. Chen Z., Wojcik S.F., Welker N.E. 1986; High frequency transformation of Bacillus stearothermophilus by protoplast fusion.. Journal of Bacteriology 165:994–1001
    [Google Scholar]
  6. Decastro-Costa M., Landman O. 1977; Inhibitory protein controls the reversion of protoplasts and L forms of Bacillus subtilis to the walled state.. Journal of Bacteriology 129:678–689
    [Google Scholar]
  7. Gonzalez J.M.JR 1982; Plasmid transfer in Bacillus thuringiensis.. In Genetic Exchange, pp 85–95 Streips U. N., Goodgal S. H., Guild W. R., Wilson G. A. Edited by New York: Marcel Dekker;
    [Google Scholar]
  8. Gray O., Chang S. 1981; Molecular cloning and expression of Bacillus licheniformis/3-lactamase gene in Escherichia coliand Bacillus subtilis.. Journal of Bacteriology 145:422–428
    [Google Scholar]
  9. Grosch J.C., Wollweber K.L. 1982; Transformation of Bacillus licheniformis and Bacillus amyloliquefaciens protoplasts by plasmid DNA.. In Genetic Exchange, pp 97–105 Streips U. N., Goodgal S. H., Guild W. R., Wilson G. A. Edited by New York: Marcel Dekker;
    [Google Scholar]
  10. Gryczan T.J., Contente S., Dubnau D. 1978; Characterization of Staphylococcus aureus plasmids, introduced by transformation into Bacillus subtilis.. Journal of Bacteriology 134:318–329
    [Google Scholar]
  11. Hulett F.M. 1987; Alkaline phosphatase from Bacillus licheniformis: proteins and genes.. In Phosphate Metabolism and Cellular Regulation in Microorganisms, pp 43–48 Torriani-Gorini A., Rothman F., Silver S., Wright A., Yagil E. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  12. Hulett F.M., Campbell L.L. 1971; Purification and properties of an alkaline phosphatase of Bacillus licheniformis.. Biochemistry 10:1364–1370
    [Google Scholar]
  13. Landman O.E., Pepin R.A. 1982; Bacillus subtilismating system: dicaryotic recombinants and suppression of the prototropic phenotype.. In Molecular Cloning and Gene Regulation in the Bacilli, pp 25–39 Ganesman A. T., Chang S., Hoch J. A. Edited by New York: Academic Press;
    [Google Scholar]
  14. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning: a laboratory Manual p. 440 Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  15. Schlief R.F., Wensink P. 1981; Working with nucleic acids.. In Practical Methods in Molecular Biology, pp 98–99 New York: Springer-Verlag;
    [Google Scholar]
  16. Spencer D.B., Chen C.-P., Hulett F.M. 1981; Effect of cobalt on synthesis and activation of Bacillus licheniformis phosphatase.. Journal of Bacteriology 145:926–933
    [Google Scholar]
  17. Van Randen J., Venema G. 1984; Direct plasmid transfer from replica plated E. coli colonies to competent Bacillus subtilis cells.. Molecular and General Genetics 195:57–61
    [Google Scholar]
/content/journal/micro/10.1099/00221287-135-8-2283
Loading
/content/journal/micro/10.1099/00221287-135-8-2283
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error