@article{mbs:/content/journal/micro/10.1099/00221287-135-8-2249, author = "Sellwood, R. and Kent, K. A. and Burrows, M. R. and Lysons, R. J. and Bland, A. P.", title = "Antibodies to a Common Outer Envelope Antigen of Treponema hyodysenteriae with Antibacterial Activity", journal= "Microbiology", year = "1989", volume = "135", number = "8", pages = "2249-2257", doi = "https://doi.org/10.1099/00221287-135-8-2249", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-135-8-2249", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Outer envelopes of Treponema hyodysenteriae strains P18A and VS1 were prepared and characterized by SDS-PAGE. In Western blot analysis of eleven strains of T. hyodysenteriae and two intestinal non-pathogenic spirochaetes, polyclonal antiserum raised to the outer envelopes of strain P18A contained antibodies primarily to two polypeptides. A 45 kDa polypeptide was present in only two strains of T. hyodysenteriae, P18A and MC52/80, whereas another antigen of 16 kDa was common to all eleven strains of T. hyodysenteriae but was not present in the two non-pathogens. Immunogold labelling of whole organisms suggested that the 16 kDa antigen was present on the surface of the spirochaetes. In in vitro tests the serum agglutinated and inhibited growth of only the T. hyodysenteriae strains, suggesting that antibodies to the 16 kDa antigen were responsible for these activities. Serum from a gnotobiotic pig infected with T. hyodysenteriae strain P18A had antibodies to the 16 kDa antigen alone and also possessed agglutinating and growth-inhibitory activities.", }