1887

Abstract

Intracellular pH (pH) was determined during arrest and recovery of temperature sensitive-cell division cycle mutants of In all mutants, pH decreased during arrest; but when the mutants were released from arrest a rapid increase in pH ensued in only - and -arrested cells. Both of these mutations cause arrest at ‘start’, the sole regulatory point in the cell cycle. In cells with or mutations, which arrest past start, pH remained constant and exhibited a decrease, respectively, upon recovery of growth. The activity of plasma membrane ATPase decreased during the first 30 min of recovery of -arrested cells, concomitant with the rise in pH. During the same period, there was no significant change in activity in -bearing cells, whereas an increase was observed for -bearing cells. Increase in pH may be used as a specific signal by for start traversal and commitment to a new cycle.

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1989-08-01
2021-08-03
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