1887

Abstract

Studies were made of the growth kinetics, morphology and phospholipid composition of two strains , a wild-type strain (A3/5) and a highly branched variant (C106) which arose spontaneously during cultivation of A3/5. No significant difference was observed between the hyphal diameters of the two strains and therefore increased branching of C106 could not be explained in the terms of an increase in hyphal radius in the absence of a change in hyphal growth unit volume. The two strains had the same specific growth rate in batch culture and this was not affected by the addition of up to 1·5 m-choline to the medium. However, choline increased the mean hyphal extension rate and colony radial growth rate of both strains and this response was correlated with the formation of mycelia which were more sparsely branched than mycelia grown on medium lacking choline. Addition of betaine, choline, ethanolamine, monomethylethanolamine or dimethylethanolamine (but not serine, glycine, dimethylglycine, methylamine, hydroxylamine or β-hydroxyethylhydrazine) to the medium also resulted in appreciable increases in the colony radial growth rates of A3/5 (increased by about 130 % for choline) and C106 (increased by about 25 % for choline). No significant difference was observed between the phospholipid compositions of the two strains, and the addition of 100 μ-choline to the medium had no significant effect on the phospholipid composition of either strain.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-8-2155
1989-08-01
2022-01-22
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/8/mic-135-8-2155.html?itemId=/content/journal/micro/10.1099/00221287-135-8-2155&mimeType=html&fmt=ahah

References

  1. Anderson C., Solomons G.L. 1984; Primary metabolism and biomass production from Fusarium.. In The Applied Mycology of Fusarium pp 231–250 Moss M. O., Smith J. E. Edited by London: British Mycological Society;
    [Google Scholar]
  2. Angus W.W., Lester R.L. 1972; Turnover of inositol and phosphorus containing lipids in Saccharomyces cerevisiae; extracellular accumulation of glycerophosphatidylinositol derived from phosphatidylinositol.. Archives of Biochemistry and Biophysics. 151:483–495
    [Google Scholar]
  3. Dittmer J.C., Lester R.L. 1964; A simple specific spray reagent for the detection of phospholipids on thin-layer chromatograms.. Journal of Lipid Research 5:126–127
    [Google Scholar]
  4. Edelman J., Fewell A., Solomons G.L. 1983; Myco-protein - a new food.. Nutrition Abstracts and Reviews 53:471–480
    [Google Scholar]
  5. Hicks C.R. 1982 Fundamental Concepts in the Design of Experiments. New York: Holt, Reinhart and Winston;
    [Google Scholar]
  6. Hubbard S.C., Brody S. 1975; Glycerophospho-lipid variation in choline and inositol auxotrophs of Neurospora crassa.. Journal of Biological Chemistry 250:7173–7183
    [Google Scholar]
  7. Humphreys A.M., Gooday G.W. 1984; Phospholipid requirement of microsomal chitinase from Mucormucedo.. Current Microbiology 11:187–190
    [Google Scholar]
  8. Jejelowo O.A., Trinci A.P.J. 1988; Effect of the paramorphogens 3-O-methylglucose, glucosamine and sorbose on growth and morphology of Botrytis fabae.. Transactions of the British Mycological Society 91:633–660
    [Google Scholar]
  9. Jukes T.H. 1947; Choline.. Annual Review of Biochemistry 16:193–222
    [Google Scholar]
  10. Kennedy E.P., Weiss S.B. 1956; The function of cytidine co-enzymes in the biosynthesis of phospholipids.. Journal of Biological Chemistry 222:193–214
    [Google Scholar]
  11. Markham P., Bainbridge B.W. 1979; Characterization of a new choline locus in Aspergillus nidulans and its significance for choline metabolism.. Genetical Research 32:303–310
    [Google Scholar]
  12. Matysiak Z., Radominska-Pyrek A., Chojnacki T. 1974; The cytidine mechanism and methylation pathway in the formation of Y-methylated ethanolamine phosphoglycerides in Neurospora crassa.. Molecular and Cellular Biochemistry 3:143–151
    [Google Scholar]
  13. Robson G.D., Kuhn P.J., Trinci A.P.J. 1988; Effects of validamycin A on the morphology, growth and sporulation of Rhizoctonia cerealis, Fusarium culmorum and other fungi.. Journal of General Microbiology 134:3187–3194
    [Google Scholar]
  14. Robson G.D., Kuhn P.J., Trinci A.P.J. 1989; Effect of validamycin A on the inositol content and branching of Rhizoctonia cerealis and other fungi.. Journal of General Microbiology 135:739–750
    [Google Scholar]
  15. Rouser G., Fleischer S., Yamamoto A. 1970; Two dimensional thin layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots.. Lipids 5:494–496
    [Google Scholar]
  16. Steele G.C., Trinci A.P.J. 1975; Morphology and growth kinetics of hyphae of differentiated and undifferentiated mycelia of Neurospora crassa.. Journal of General Microbiology 91:362–368
    [Google Scholar]
  17. Strange R.N., Smith H. 1978; Specificity of choline and betaine as stimulants of Fusarium graminearum.. Transactions of the British Mycotogical Society 70:187–192
    [Google Scholar]
  18. Strange R.N., Majer J.R., Smith H. 1974; The isolation and identification of choline and betaine as the two major components in anthers and wheat germ that stimulate Fusarium graminearum in vitro.. Physiological Plant Pathology 4:277–290
    [Google Scholar]
  19. Trinci A.P.J. 1972; Culture turbidity as a parameter of mould growth.. Transactions of the British Mycological Society 58:467–473
    [Google Scholar]
  20. Trinci A.P.J. 1974; A study of the kinetics of hyphal extension and branch initiation of fungal mycelia.. Journal of General Microbiology 81:225–236
    [Google Scholar]
  21. Trinci A.P.J. 1984; Antifungal agents which affect hyphal extension and hyphal branching.. In Mode of Action of Antifungal Agents, pp 113–134 Trinci A. P. J., Ryley J. F. Edited by London: British Mycological Society;
    [Google Scholar]
  22. Trinci A.P.J., Collinge A. 1973; Influence of L-sorbose on growth and morphology of Neurospora crassa.. Journal of General Microbiology 78:179–192
    [Google Scholar]
  23. Vermeulen C.A., Wessels J.G.H. 1983; Evidence for a phospholipid requirement of chitin synthase in Schizophyllum commune.. Current Microbiology 8:67–71
    [Google Scholar]
  24. Vogel H.J. 1956; A convenient growth medium for Neurospora (Medium N).. Microbial Genetics Bulletin 13:42–44
    [Google Scholar]
  25. Wilson A.C., Barran L.R. 1983; Effect of temperature on phosphatidylcholine synthesis by hyphae of Fusarium oxysporumf.sp.lycopersici.. Transactions of the British Mycological Society 80:113–116
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-8-2155
Loading
/content/journal/micro/10.1099/00221287-135-8-2155
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error