1887

Abstract

Staphylococcal DNA was digested with endonucleases and probed with labelled ribosomal RNA (rRNA) from Reproducible restriction patterns containing between seven and 22 bands were obtained for seven different species of staphylococci. These profiles were species-specific with different strains of a particular species sharing an identical or similar restriction pattern. The results reported here indicate that rRNA gene restriction pattern analyses have an application in the taxonomy of staphylococci.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-7-2093
1989-07-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/7/mic-135-7-2093.html?itemId=/content/journal/micro/10.1099/00221287-135-7-2093&mimeType=html&fmt=ahah

References

  1. Archer G.L., Karchmer A.W., Vishniavsky N., Johnston J.L. 1984; Plasmid pattern analysis for the differentiation of infecting from non-infecting Staphylococcus epidermidis.. Journal of Infectious Diseases 149:913–920
    [Google Scholar]
  2. Clink J., Pennington T.H. 1986; Staphylococcal whole-cell polypeptide analysis: evaluation as a taxonomic and typing tool.. Journal of Medical Microbiology 22:41–44
    [Google Scholar]
  3. Gelmi M., Foresti I., Ravizzola G., Bonfanti C., Verardi R., Caruso A., Turano A. 1987; Antibiotic resistance and plasmids from Staphylococcus aureus from Italian hospitals.. Journal of Medical Microbiology 23:111–118
    [Google Scholar]
  4. Grimont F., Grimont P.A.D. 1986; Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools.. Annales de l’Institut PasteurjMicrobiologie 137B:165–175
    [Google Scholar]
  5. Irino K., Grimont F., Casin I., Grimont P.A.D.The Brazilian Purpuric Fever Study Group 1988; rRNA gene restriction patterns of Haemophilus influenzae biogroup aegyptius strains associated with Brazilian purpuric fever.. Journal of Clinical Microbiology 26:1535–1538
    [Google Scholar]
  6. Johnson J.L. 1985; DNA reassociation and RNA hybridization of bacterial nucleic acids.. Methods in Microbiology 18:33–74
    [Google Scholar]
  7. Kloos W.E., Schleifer K.H. 1975; Simplified scheme for routine identification of human Staphylococcus species.. Journal of Clinical Microbiology 1:82–88
    [Google Scholar]
  8. Kocur M., Bergan T., Mortensen N. 1971; DNA base composition of Gram-positive cocci.. Journal of General Microbiology 69:167–183
    [Google Scholar]
  9. Magee B.B., D’Souza T.M., Magee P.T. 1987; Strain and species identification by restriction fragment length polymorphisms in the ribosomal DNA repeat of Candida species.. Journal of Bacteriology 169:1639–1643
    [Google Scholar]
  10. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  11. Owen R.J., Beck A., Dayal P.A., Dawson C. 1988; Detection of genomic variation in Providen- cia stuartii clinical isolates by analysis of DNA restriction fragment length polymorphisms containing rRNA cistrons.. Journal of Clinical Microbiology 26:2161–2166
    [Google Scholar]
  12. Desaxe M.J., Crees-Morris J.A., Marples R.R., Richardson J.F. 1981; Evaluation of current phage-typing systems for coagulase-negative staphylococci.. Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene suppl. 10:197–204
    [Google Scholar]
  13. Schito G.C., Varaldo P.E. 1988; Trends in the epidemiology and antibiotic resistance of clinical Staphylococcus strains in Italy - a review.. Journal of Antimicrobial Chemotherapy 21: suppl.C 67–78
    [Google Scholar]
  14. Southern E. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis.. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  15. Thomson-Carter F.M., Pennington T.H. 1989; Characterisation of methicillin-resistant isolates of Staphylococcus aureus by analysis of whole-cell and exported proteins.. Journal of Medical Microbiology 28:25–32
    [Google Scholar]
  16. Vindel A., Martin-Bourgon C., Saez-Nieto J.A. 1987; Characterization of non-typable strains of Staphylococcus aureus from cases of hospital infection.. Epidemiology and Infection 99:191–200
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-7-2093
Loading
/content/journal/micro/10.1099/00221287-135-7-2093
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error