1887

Abstract

SUMMARY: T-2 completely converted -toluenesulphonic acid (TS) or -sulphobenzoic acid (PSB) to cell material, CO and sulphate, with growth yields of about 5 g protein (mol C). PSB and sulphite were excreted as transient intermediates during growth in TS-salts medium. All reactions of a catabolic pathway involving sidechain oxidation and cleavage of the sulphonate moiety as sulphite were measurable in the soluble portion of cell extracts. Degradation of TS and PSB was inducible and apparently involved at least two regulons. TS was converted to -sulphobenzyl alcohol in a reaction requiring NAD(P)H and 1 mol O (mol TS). This alcohol was in an equilibrium (in the presence of NAD) with -sulphobenzaldehyde, which was converted to PSB in an NAD(P)-dependent reaction. PSB was desulphonated to protocatechuic acid in a reaction requiring NAD(P)H and 1 mol O (mol PSB). Experiments with O confirmed involvement of a dioxygenase, because both atoms of this molecular oxygen were recovered in protocatechuate. Protocatechuate was converted to 2-hydroxy-4-carboxymuconate semialdehyde by a 4,5-dioxygenase.

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/content/journal/micro/10.1099/00221287-135-7-1969
1989-07-01
2019-10-17
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-7-1969
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