1887

Abstract

The total lipid content of (serotype A; NCPF 3153) exponential-phase mycelial cultures grown in tissue-culture medium 199 (containing 10%, v/v, foetal calf serum) was 29·8 ± 8 mg (g dry weight) (mean ± ). The weight ratios of phospholipid to neutral lipid and phospholipid to non-esterified sterol were 2·6 ± 0·4 and 24·9 ± 0·5, respectively. The major phospholipid was phosphatidylcholine with smaller amounts of phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidylglycerol and diphosphatidylglycerol; the most abundant fatty acids were palmitic, palmitoleic, oleic and linoleic acids. The major neutral lipids comprised esterified sterol, triacylglycerol and non-esterified fatty acid with a smaller amount of non-esterified sterol. The fatty acid compositions of the three fatty-acid-containing neutral lipids were distinct from each other and the phospholipids. Comparison with previous data on yeast cultures of C. A grown in glucose broth shows that mycelial cultures have a larger lipid content, lower phospholipid to neutral lipid ratio and higher phospholipid to non-esterified sterol ratio. We now show that mycelial cultures were more permeable to a [C]triazole antifungal antibiotic compared with exponentially growing yeast cultures of several azole-sensitive strains. Taken together these data are consistent with there being a relationship between the phospholipid/non-esterified sterol ratio of a culture and its ability to accumulate a triazole.

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/content/journal/micro/10.1099/00221287-135-7-1949
1989-07-01
2021-08-03
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References

  1. Bligh E.G., Dyer W.J. 1959; A rapid method of total lipid extraction and purification.. Canadian Journal of Biochemistry and Physiology 37:911–917
    [Google Scholar]
  2. Boiron P., Drouhet E., Dupont B., Improvisi L. 1987; Entry of ketoconazole into Candida albicans.. Antimicrobial Agents and Chemotherapy 31:244–248
    [Google Scholar]
  3. Borgers M. 1985; Antifungal Azole Derivatives.. In The Scientific Basis of Antimicrobial Chemotherapy pp 133–153 Greenwood D., O'Grady F. Edited by Cambridge: Cambridge University Press;
    [Google Scholar]
  4. Braun P.C. 1978; Chitin synthesis in Candida albicans ', comparison of yeast and hyphal forms.. Journal of Bacteriology 133:1472–1477
    [Google Scholar]
  5. Christie W.W. 1982 Lipid Analysis, 2nd edn.. Oxford: Pergamon Press;
    [Google Scholar]
  6. Davies R.R., Denning T.J.V. 1972; Growth and form in Candida albicans.. Sabouraudia 10:180–188
    [Google Scholar]
  7. Fromtling R.A. 1988; Overview of medically important antifungal azole derivatives.. Clinical Microbiology Reviews 1:187–217
    [Google Scholar]
  8. Ghannoum M.A., Janini G., Khamis L., Radwan S.S. 1986; Dimorphism-associated variations in the lipid composition of Candida albicans.. Journal of General Microbiology 132:2367–2375
    [Google Scholar]
  9. Grover A.K., Cushley R.J. 1979; Cholesterol ester distribution in lecithin bilayer membranes.. Atherosclerosis 32:87–91
    [Google Scholar]
  10. Hitchcock C.A., Barrett-Bee K.J., Russell N.J. 1986; The lipid composition of azole-sensitive and azole-resistant strains of Candida albicans.. Journal of General Microbiology 132:2421–2431
    [Google Scholar]
  11. Hitchcock C.A., Barrett-Bee K.J., Russell N.J. 1987a; The lipid composition and permeability to azole of an azole- and polyene-resistant mutant of Candida albicans.. Journal of Medical and Veterinary Mycology 25:29–37
    [Google Scholar]
  12. Hitchcock C.A., Russell N.J., Barrett-Bee K.J. 1987b; Sterols in Candida albicans mutants resistant to polyene or azole antifungals, and of a double mutant C. albicans 6.4.. CRC Critical Reviews in Microbiology 15:111–115
    [Google Scholar]
  13. Leyva A., Kelley W.N. 1974; Measurement of DNA in cultured human cells.. Analytical Biochemistry 62:173–179
    [Google Scholar]
  14. Marriott M.S. 1975; Isolation and chemical characterization of plasma membranes from the yeast and mycelial forms of Candida albicans.. Journal of General Microbiology 86:115–132
    [Google Scholar]
  15. Odds F.C. 1980; Laboratory evaluation of antifungal agents: a comparative study of five imidazole derivatives of clinical importance.. Journal of Antimicrobial Chemotherapy 6:749–761
    [Google Scholar]
  16. Odds F.C. 1985; Morphogenesis in Candida albicans.. CRC Critical Reviews in Microbiology 12:45–93
    [Google Scholar]
  17. Odds F.C. 1988 Candida and Candidosis, 2nd edn.. London: Balliere Tindall;
    [Google Scholar]
  18. Russell N.J. 1989; Functions of lipids: structural roles and membrane functions.. In Microbial Lipids 2 ch. 17. Ratledge C., Wilkinson S. G. Edited by London: Academic Press; (in the Press)
    [Google Scholar]
  19. Ryley J.F., Wilson R.G., Barrett-Bee K.J. 1984; Azole resistance in Candida albicans.. Sabouraudia 22:53–63
    [Google Scholar]
  20. Sandermann H. 1978; Regulation of membrane enzymes by lipids.. Biochimica et biophysica acta 515:209–237
    [Google Scholar]
  21. Shimokawa O., Kato Y., Nakayama H. 1986; Increased drug sensitivity in Candida albicans cells accumulating 14-methylated sterols.. Journal of Medical and Veterinary Mycology 24:481–483
    [Google Scholar]
  22. Sundaram S., Sullivan P.A., Shepherd M.G. 1981; Changes in lipid composition during starvation and germ-tube formation in Candida albicans.. Experimental Mycology 5:140–147
    [Google Scholar]
  23. Van den bossche H.V., Willemsens G., Cools W., Cornelissen F., Lauwers W.F., Van cutsem J.M. 1980; In vitro and in vivo effects of the antimycotic drug ketoconazole on sterol biosynthesis.. Antimicrobial Agents and Chemotherapy 17:922–928
    [Google Scholar]
  24. Van den bossche H.V., Willemsens G., Cools W., Marichal P., Lauwers W. 1983; Hypothesis on the molecular basis of the antifungal activity of V-substituted imidazoles and triazoles.. Biochemical Society Transactions 11:665–666
    [Google Scholar]
  25. Wells M.A., Dittmer J.C. 1963; The use of Sephadex for the removal of nonlipid contaminants from lipid extracts.. Biochemistry 2:1259–1263
    [Google Scholar]
  26. Yamaguchi H. 1974; Mycelial development and chemical alteration of Candida albicans from biotin insufficiency.. Sabouraudia 12:320–328
    [Google Scholar]
  27. Yano K., Yamada T., Banno Y., Sekiya T., Nozawa Y. 1982; Modification of lipid composition in a dimorphic fungus, Candida albicans during the yeast to hypha transformation.. Japanese Journal of Medical Mycology 23:159–165
    [Google Scholar]
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