1887

Abstract

The formation of gas vesicles in samples taken from phosphorus-limited cultures was studied after a phosphate pulse. A light period of at least 10 h after the pulse was needed before gas vesicles were synthesized , and formation did not occur in the dark. The length of the light period correlated with the increase in gas vacuolation. When samples were subjected to 5 h light periods at different times after the addition of phosphate, formation of gas vesicles was only observed when the light period started at least 5 h after the addition of phosphate. Gas vesicle formation was saturated at a photon flux density of approximately 50 μmol m s. Synthesis of gas vesicles was not detected when the cultures were treated with chloramphenicol, rifampicin or 3-(3,4-dichlorophenyl)-′-dimethylurea (DCMU); total gas vesicle volume did not decrease under these conditions, suggesting that turnover of gas vesicles occurs very slowly, if at all.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-7-1933
1989-07-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/7/mic-135-7-1933.html?itemId=/content/journal/micro/10.1099/00221287-135-7-1933&mimeType=html&fmt=ahah

References

  1. Armstrong R.E., Hayes P.K., Walsby A.E. 1983; Gas vacuole formation in hormogonia of Nostocmuscorum.. Journal of General Microbiology 129:263–270
    [Google Scholar]
  2. Damerval T., Houmard J., Guglielmi G., Csizar K., Tandeau De Marsac N. 1987; A developmentally regulated gvpABC operon is involved in the formation of gas vesicles in the cyanobacteriumCalothrix 7601.. Gene 54:83–92
    [Google Scholar]
  3. Hayes P.K., Walsby A.E. 1984; An investigation into the recycling of gas vesicle protein derived from collapsed gas vesicles.. Journal of General Microbiology 130:1591–1596
    [Google Scholar]
  4. Herbert D., Phipps P.J., Strange R.W. 1971; Chemical analyses of microbial cells.. Methods in Microbiology 5B:209–344
    [Google Scholar]
  5. Jones D.D., Jost M. 1970; Isolation and chemical characterization of gas-vacuole membranes from Microcystis aeruginosaKuetz emend. Elenkin.. Archiv für Mikrobiologie 70:43–64
    [Google Scholar]
  6. Klemer A.R. 1976; The vertical distribution of Oscillatoria agardhii var. isothrix.. Archiv für Hydrobiologie 78:343–362
    [Google Scholar]
  7. Klemer A.R. 1978; Nitrogen limitation of growth and gas vacuolation in Oscillatoria rubescens.. Verhandlungen der Internationalen Vereinigung für theoretische und angewandte Limnologie 20:2293–2297
    [Google Scholar]
  8. Konopka A.E., Staley J.T., Lara J.C. 1975; Gas vesicle assembly in Microcyclus aquaticus.. Journal of Bacteriology 122:1301–1309
    [Google Scholar]
  9. Konopka A., Lara J.C., Staley J.T. 1977; Isolation and characterization of gas vesicles from Microcyclus aquaticus.. Archives of Microbiology 112:133–140
    [Google Scholar]
  10. Konopka A., Kromkamp J., Mur L.R. 1987a; Regulation of gas vesicle content and buoyancy in light- or phosphate-limited cultures of Aphanizomenon flos-aquae (Cyanophyceae).. Journal of Phycology 23:70–78
    [Google Scholar]
  11. Konopka A., Kromkamp J.C., Mur L.R. 1987b; Buoyancy regulation in phosphate-limited cultures of Microcystis aeruginosa.. FEMS Microbiology Ecology 45:135–142
    [Google Scholar]
  12. Kromkamp J.C., Mur L.R. 1984; Buoyant density changes in the cyanobacterium Microcystis aeruginosa due to changes in the cellular carbohydrate content.. FEMS Microbiology Letters 25:105–109
    [Google Scholar]
  13. Kromkamp J., Konopka A., Mur L.R. 1986; Buoyancy regulation in a strain of Aphanizomenon flos-aquae (Cyanophyceae): the importance of carbohydrate accumulation and gas vesicle collapse.. Journal of General Microbiology 132:2113–2121
    [Google Scholar]
  14. Kromkamp J., Konopka A., Mur L.R. 1988; Buoyancy regulation in light-limited continuous cultures of Microcystis aeruginosa.. Journal of Plankton Research 10:171–183
    [Google Scholar]
  15. Lehmann H., Jost M. 1971; Kinetics of the assembly of gas vacuoles in the blue-green alga Microcystis aeruginosaKuetz emend. Elenkin.. Archiv für Mikrobiologie 79:59–68
    [Google Scholar]
  16. Oliver R.L., Walsby A.E. 1984; Direct evidence for the role of light-mediated gas-vesicle collapse in the buoyancy regulation of Anabaena flos-aquae(cyanobacteria).. Limnology and Oceanography 29:879–886
    [Google Scholar]
  17. Raj A.D. 1983; Proposal of Ancylobacter gen. nov., as a substitute for the bacterial genus Microcyclus Orskov 1928.. International Journal of Systematic Bacteriology 33:397–398
    [Google Scholar]
  18. Waaland J.R., Branton D. 1969; Gas vacuole development in a blue-green alga.. Science 163:1339–1341
    [Google Scholar]
  19. Walker J.E., Walsby A.E. 1983; Molecular weight of gas-vesicle protein from the planktonic cyanobacterium Anabaena flos-aquae and implications for the structure of the vesicles.. Biochemical Journal 209:809–815
    [Google Scholar]
  20. Walsby A.E. 1973; A portable apparatus for measuring relative gas vacuolation, the strength of gas vacuoles and turgor pressure in planktonic blue-green algae and bacteria.. Limnology and Oceanography 18:653–658
    [Google Scholar]
  21. Walsby A.E. 1982; The elastic compressibility of gas vesicles.. Proceedings of the Royal Society London B216:335–368
    [Google Scholar]
  22. Walsby A.E., Buckland B. 1969; Isolation and purification of intact gas vesicles from a blue-green alga.. Nature; London: 224716–717
    [Google Scholar]
  23. Zehnder A., Hughes E.O. 1958; The anti-algal activity of actidione.. Canadian Journal of Microbiology 4:399–408
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-7-1933
Loading
/content/journal/micro/10.1099/00221287-135-7-1933
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error