1887

Abstract

Phototaxis by slugs was disoriented in the presence of pertussis toxin, suggesting a role for pertussis-toxin-sensitive G-proteins in slug behaviour. The decrease in orientation accuracy was dependent on the concentration of this bacterial toxin. Pertussis toxin did not significantly affect slug thermotaxis, while cholera toxin had no significant effects on either slug phototaxis or thermotaxis. The addition of micromolar concentrations of lithium salts (LiCl or LiBr) resulted in disorientation of both phototaxis and positive thermotaxis by slugs. The corresponding sodium salts had no effect on slug behaviour, confirming that the effects were specific to lithium ions. The effects of lithium ions suggest a role for inositol phosphates as possible second messengers in slug behaviour. Except for slight impairment of thermotaxis by -sphingosine, we found no effects on slug behaviour of either phorbol 12-myristate 13-acetateorsphingolipids. We thus found little pharmacological evidence for a role in slug signal transduction of the diacylglycerol branch of the inositol lipid pathway.

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1989-07-01
2021-10-25
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References

  1. Dohrmann U., Fisher P.R., Brüderlein M., Williams K.L. 1984a; Transitions in Dictyostelium discoideum behaviour: influence of calcium and fluoride on slug phototaxis and thermotaxis.. Journal of Cell Science 65:111–121
    [Google Scholar]
  2. Dohrmann U., Fisher P.R., Brüderlein M., Lackner B., Williams K.L. 1984b; Arachi-donic acid and related molecules affect the behaviour of Dictyostelium discoideum slugs.. Journal of General Microbiology 130:2685–2698
    [Google Scholar]
  3. Europe-Finner G.N., Newell P.C. 1985; Inositol 1,4,5-triphosphate induces cyclic GMP formation in Dictyostelium discoideum.. Biochemical and Biophysical Research Communications 130:1115–1122
    [Google Scholar]
  4. Europe-Finner G.N., Newell P.C. 1986a; Inositol 1,4,5-triphosphate induces calcium release from a nonmitochondrial pool in amoebae of Dictyostelium.. Biochimica et biophysica acta 887:335–340
    [Google Scholar]
  5. Europe-Finner G.N., Newell P.C. 1986b; Inositol 1,4,5-triphosphate and calcium stimulate actin polymerization in Dictyostelium discoideum.. Journal of Cell Science 82:41–51
    [Google Scholar]
  6. Europe-Finner G.N., Newell P.C. 1987; GTP analogues stimulate inositol triphosphate formation in Dictyostelium.. Journal of Cell Science 87:513–518
    [Google Scholar]
  7. Europe-Finner G.N., Ludérus M.E.E., Small N.V., vanDriel R., Reymond C.D., Firtel R.A., Newell P.C. 1988; Mutant ras gene induces elevated levels of inositol tris- and hexakis- phosphates in Dictyostelium.. Journal of Cell Science 89:13–20
    [Google Scholar]
  8. Fisher P.R., Williams K.L. 1982; Thermotactic behaviour of Dictyostelium discoideum slug phototaxis mutants.. Journal of General Microbiology 128:965–971
    [Google Scholar]
  9. Fisher P.R., Smith E., Williams K.L. 1981; An extracellular chemical signal controlling phototactic behaviour by D. discoideum slugs.. Cell 23:799–807
    [Google Scholar]
  10. Fisher P.R., Dohrmann U., Williams K.L. 1984; Signal processing in Dictyostelium discoideumslugs.. Modern Cell Biology: A Review Series 3:197–248
    [Google Scholar]
  11. Gerisch G. 1987; Cyclic AMP and other signals controlling development and differentiation in Dictyostelium.. Annual Review of Biochemistry 56:853–879
    [Google Scholar]
  12. Hannun Y.A., Bell R.M. 1987; Lysosphingo- lipids inhibit protein kinase C: implications for the sphingolipidoses.. Science 235:670–673
    [Google Scholar]
  13. Jannsens P.M.W., vanHaastert P.J.M. 1987; Molecular basis of transmembrane signal transduction in Dictyostelium discoideum.. Microbiological Reviews 51:396–418
    [Google Scholar]
  14. Kesbeke F., Snaar-Jagalska B.E., vanHaastert P.J.M. 1988; Signal transduction in Dictyostelium fgdA mutants with a defective interaction between surface cAMP receptor and a GTP- binding regulatory protein.. Journal of Cell Biology 107:521–528
    [Google Scholar]
  15. Kikkawa U., Nishizuka Y. 1986; The role of protein kinase C in transmembrane signalling.. Annual Review of Cell Biology 2:149–178
    [Google Scholar]
  16. Leichtling B.H., Coffman D.S., Yaeger E.S., Rickenberg H. 1981; Occurrence of the adenylate cyclase ‘G-protein’ in membranes of Dictyostelium discoideum.. Biochemical and Biophysical Research Communications 102:1187–1195
    [Google Scholar]
  17. Leitz T., Muller W.A. 1986; Evidence for the involvement of PI-signalling and diacyl glycerol second messengers in the initiation of metamorphosis in the hydroid Hydractinia echinata Fleming.. Developmental Biology 121:82–89
    [Google Scholar]
  18. vanLookerencampagne M.M., Erneux C., Vaneijk R., vanHaastert P.J.M. 1988; Two dephosphorylation pathways of inositol 1,4,5-triphosphate in Dictyostelium discoideum homogenates.. Biochemical Journal 254:343–350
    [Google Scholar]
  19. Loomis W.F. (editor) 1982 The Development of Dictyostelium discoideum. New York:: Academic Press.;
    [Google Scholar]
  20. Ludérus M.E.E., Reymond C.D., vanHaastert P.J.M., vanDriel R. 1988; Expression of a mutated ras gene in Dictyostelium discoideum alters the binding of cyclic AMP to its chemotactic receptor.. Journal of Cell Science 90:701–706
    [Google Scholar]
  21. McRobbie S.J. 1986; Chemotaxis and cell motility in the cellular slime moulds.. CRC Critical Reviews in Microbiology 13:335–375
    [Google Scholar]
  22. Newell P.C., Europe-Finner G.N., Small N.V., Liu G. 1988; Inositol phosphates, G-proteins and ras genes involved in chemotactic signal transduction of Dictyostelium discoideum.. Journal of Cell Science 89:123–127
    [Google Scholar]
  23. Snaar-Jagalska B.E., Jokabs K.H., vanHaastert P.J.M. 1988; Agonist stimulated high affinity GTP-ase in Dictyostelium membranes.. FEBS Letters 236:139–144
    [Google Scholar]
  24. Sternweis P.C., Gilman A.G. 1982; Aluminum: a requirement for activation of the regulatory component of adenylate cyclase by fluoride.. Proceedings of the National Academy of Sciences of the United States of America 79:4888–4891
    [Google Scholar]
  25. Stryer L., Bourne H.R. 1986; G proteins: a family of signal transducers.. Annual Review of Cell Biology 2:391–419
    [Google Scholar]
  26. Thiery R., Klein R., Tatischeff I. 1988; Phorbol 12-myristate 13-acetate modulates the cAMP-induced light-scattering response of a Dictyostelium discoideum cell population.. FEBS tetters 241:149–153
    [Google Scholar]
  27. Worley P.F., Heller W.A., Synder S.H., Baraban J.M. 1988; Lithium blocks a phospho- inositode-mediated cholinergic response in hippocampal slices.. Science 239:1428–1429
    [Google Scholar]
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