Relationship between Structure and Antigenicity of O1 Lipopolysaccharides Free

Abstract

The relationship between the release of fructose from 01 lipopolysaccharides (LPS) by dilute acetic acid hydrolysis and the decrease in their antigenicity was examined. Decrease in the intigenicity of LPS was not parallel with the release of fructose, and occurred very much later than the latter. Periodate oxidation of LPS resulted in the total elimination of the fructose and glucose, and two-thirds of the heptose constituents, but no difference in the antigenicity of LPS was observed before and after oxidation. These findings indicate that the fructose present in O1 LPS is not substantially involved in their specific antigenicity. In the O1 LPS, the fructose is in the branch structure, most probably in the core region.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-7-1901
1989-07-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/7/mic-135-7-1901.html?itemId=/content/journal/micro/10.1099/00221287-135-7-1901&mimeType=html&fmt=ahah

References

  1. Bagdian G., Droge W., Kotelko K., Luderitz O., Westphal O. 1966; Vorkommen zweier Hepto- sen in Lipopolysacchariden enterobacterieller Zell- wande: L-glycero- und D-glycero-D-mannoheptose.. Biochemische Zeitschrift 344:197–211
    [Google Scholar]
  2. Brade H., Galanos C. 1983a; A new lipopolysac- charide antigen identified in Acinetobacter calcoace- ticus: occurrence of widespread natural antibody.. Journal of Medical Microbiology 16:203–210
    [Google Scholar]
  3. Brade H., Galanos C. 1983b; Common lipopoly- saccharide specificity: new type of antigen residing in the inner core region of S- and R-form lipopolysac- charides from different families of gram-negative bacteria.. Infection and Immunity 42:250–256
    [Google Scholar]
  4. Chaby R., Szabo L. 1975; 3-Deoxy-2-octulosonic acid 5-phosphate: a component of the endotoxin of Bordetella pertussis.. European Journal of Biochemistry 59:277–280
    [Google Scholar]
  5. Finkelstein R.A. 1973; Cholera.. CRC Critical Reviews in Microbiology 2:553–623
    [Google Scholar]
  6. Freeman G.G. 1942; The preparation and properties of a specific polysaccharide from Bad. typhosum Ty2.. Biochemical Journal 36:340–355
    [Google Scholar]
  7. Hisatsune K., Kondo S. 1980; Lipopolysacchar- ides of R mutants isolated from Vibrio cholerae.. Biochemical Journal 185:77–81
    [Google Scholar]
  8. Hisatsune K., Kondo S., Kobayashi K. 1978; Lipopolysaccharides of Vibrio cholerae. (II). An immunochemical study on O-antigenic structure.. Japanese Journal of Medical Science and Biology 31:181–184
    [Google Scholar]
  9. Hisatsune K., Yamamoto F., Kondo S. 1985; Lipopolysaccharide of Vibrio cholerae: chemical and serological properties.. In Advances in Research on Cholera and Related Diarrheas pp. 17–24 Kuwahara S., Pierce N.F. Edited by Tokyo: KTK Scientific Publishers;
    [Google Scholar]
  10. Jann B., Jann K., Beyaert G.O. 1973; 2-Amino-2,6-dideoxy-D-glucose (D-quinovosamine): a constituent of the lipopolysaccharides of Vibrio cholerae.. European Journal of Biochemistry 37:531–534
    [Google Scholar]
  11. Kaca W., Brade L., Rietschel E.T., Brade H. 1986; The effect of removal of D-fructose on the antigenicity of the lipopolysaccharide from a rough mutant of Vibrio cholerae Ogawa.. Carbohydrate Research 149:293–298
    [Google Scholar]
  12. Kaca W., Jough-Leuvenink J., Zahringer U., Rietschel Th E., Brade H. 1988; Isolation and chemical analysis of 7-0-(2-amino-2-deoxy-D-gluco- pyranosyl)-L-glycero-D-manno-heptose as a constituent of the lipopolysaccharides of the UDP-galactose epimeraseless mutantJ-5 of Escherichia coli and Vibrio cholerae.. Carbohydrate Research 179:289–299
    [Google Scholar]
  13. Kenne L., Lindberg B. 1983; Bacterial polysaccharides.. In The Polysaccharides 2 pp. 287–363 Aspinall G.O. Edited by New York: Academic Press;
    [Google Scholar]
  14. Kondo S., Iguchi T., Hisatsune K. 1988; A comparative study of the sugar composition of lipopolysaccharides isolated from Vibrio cholerae,'Vibrio albensis' and Vibrio metschnikovii.. Journal of General Microbiology 134:1699–1705
    [Google Scholar]
  15. OSBORN M.J. 1963; Studies on the gram-negative cell walls. I. Evidence for the role of 2-keto-3-deoxyoctonate in the lipopolysaccharide of Salmonella typhimurium.. Proceedings of the National Academy of Sciences of the United States of America 50:499–506
    [Google Scholar]
  16. Redmond J.W., Korsch M.J. 1973; Immunochemical studies of the O-antigens of Vibrio cholerae: partial characterization of an acid-labile antigenic determinant.. Australian Journal of Experimental Biology and Medical Science 51:229–235
    [Google Scholar]
  17. Rodriguez M.L., Jann B., Jann K. 1988; Structure and serological characteristics of the capsular K4 antigen of Escherichia coli 05: K4: H4, a fructose-containing polysaccharide with a chondroi- tin backbone.. European Journal of Biochemistry 111:117–124
    [Google Scholar]
  18. Sakazaki R., Tamura K. 1971; Somatic antigen variation in Vibrio cholerae.. Japanese Journal of Medical Science and Biology 4:93–100
    [Google Scholar]
  19. Shimada T., Sakazaki R. 1973; R antigen of Vibrio cholerae.. Japanese Journal of Medical Science and Biology 26:155–160
    [Google Scholar]
  20. Weissbach A., Hurwitz J. 1959; The formation of 2-keto-3-deoxy-heptonic acid in extracts of Escherichia coli B.. Journal of Biological Chemistry 234:705–709
    [Google Scholar]
  21. Westphal O., Luderitz O., Bister R. 1952; Uber die Extraction von Bakterien mit Phenol/ Wasser.. Zeitschrift für Naturforschung 7b:148–155
    [Google Scholar]
  22. Wilkinson S.G. 1977; Composition and structure of bacterial lipopolysaccharides.. In surface Carbohydrates of the Prokaryotic Cell pp. 97–175 Sutherland I.W. Edited by New York: Academic Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-7-1901
Loading
/content/journal/micro/10.1099/00221287-135-7-1901
Loading

Data & Media loading...

Most cited Most Cited RSS feed