@article{mbs:/content/journal/micro/10.1099/00221287-135-7-1847, author = "Diaz, Luis A. and Hardisson, Carlos and Rodicio, M. Rosario", title = "Isolation and Characterization of Actinophages Infecting Streptomyces Species and Their Interaction with Host Restriction-Modification Systems", journal= "Microbiology", year = "1989", volume = "135", number = "7", pages = "1847-1856", doi = "https://doi.org/10.1099/00221287-135-7-1847", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-135-7-1847", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Nine different phages, øA1 to øA9, were isolated from soil samples on Streptomyces antibioticus ATCC 11891, a strain which produces the macrolide antibiotic oleandomycin. Each phage displayed a different host-range which did not extend beyond Streptomyces species. Host-range was mainly limited by adsorption specificity and host-controlled restriction-modification systems. All the phages except øA3 and øA9 formed turbid plaques on S. antibioticus, but did not lysogenize this host. However, three of the phages (øA5, øA7 and øA8) were identified as temperate, since they were able to lysogenize other Streptomyces strains. All of the phages were morphologically similar and belonged to group B of Bradley’s classification. They had polyhedral heads and long, non-contractile tails. øA5, øA6 and øA7 had a base plate at the terminal end of the tail. Analysis with restriction endonucleases indicated that the nine phages contained double-strandpd DNA. Hybridization studies between the phage genomes, together with results on genome structure, allowed classification of the phages into five groups: (I) øA2, øA4 and øA9, (II) øA3 and øA8, (III) øA7, (IV) øA5 and øA6, and (V) øA1.", }