SUMMARY: Nine different phages, A1 to φA9, were isolated from soil samples on ATCC 11891, a strain which produces the macrolide antibiotic oleandomycin. Each phage displayed a different host-range which did not extend beyond species. Host-range was mainly limited by adsorption specificity and host-controlled restriction-modification systems. All the phages except φA3 and φA9 formed turbid plaques on , but did not lysogenize this host. However, three of the phages (φA5, φA7 and φA8) were identified as temperate, since they were able to lysogenize other strains. All of the phages were morphologically similar and belonged to group B of Bradley's classification. They had polyhedral heads and long, non-contractile tails. φA5, φA6 and φA7 had a base plate at the terminal end of the tail. Analysis with restriction endonucleases indicated that the nine phages contained double-strandpd DNA. Hybridization studies between the phage genomes, together with results on genome structure, allowed classification of the phages into five groups: (I) φA2, φA4 and φA9, (II) φA3 and φA8, (III) φA7, (IV) φA5 and φA6, and (V) φA1.


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