SUMMARY: The gene coding for protein A () has been mapped close to on the genetic map of the chromosome of 8325-4. A rapid mapping procedure has been developed which first allowed the region of the chromosome carrying the gene to be identified by blot hybridization of large DNA fragments which had been separated by pulsed-field gel electrophoresis. Restriction endonuclease fragment G was shown to carry the gene. An insertion mutation in was constructed by insertion of a fragment of DNA expressing resistance to kanamycin and neomycin. A ::KanNeo mutation was isolated in . 8325-4 by allele replacement. This provided a selectable marker which allowed the gene to be mapped by transformation analysis.


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