1887

Abstract

The gene coding for protein A () has been mapped close to on the genetic map of the chromosome of 8325–4. A rapid mapping procedure has been developed which first allowed the region of the chromosome carrying the gene to be identified by blot hybridization of large DNA fragments which had been separated by pulsed-field gel electrophoresis. Restriction endonuclease fragment G was shown to carry the gene. An insertion mutation in was constructed by insertion of a fragment of DNA expressing resistance to kanamycin and neomycin. A ::KanNeo mutation was isolated in . 8325–4 by allele replacement. This provided a selectable marker which allowed the gene to be mapped by transformation analysis.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-7-1799
1989-07-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/7/mic-135-7-1799.html?itemId=/content/journal/micro/10.1099/00221287-135-7-1799&mimeType=html&fmt=ahah

References

  1. Asheshov E.H. 1966; Loss of antibiotic resistance in Staphylococcus aureus resulting from growth at high temperature. Journal of General Microbiology 42:403–410
    [Google Scholar]
  2. Betley M.J., Mekalanos J.J. 1985; Staphylococcal enterotoxin A is encoded by a phage. Science 229:185–187
    [Google Scholar]
  3. Coleman D.C., Pomeroy H., Estridge J.K., Keane C.T., Cafferkey M.T., Hone R., Foster T.J. 1985; Susceptibility to antimicrobial agents and analysis of plasmids in gentamicin- and methicillin-resistant Staphylococcus aureus from Dublin hospitals. Journal of Medical Microbiology 20:157–167
    [Google Scholar]
  4. Evans J., Dyke K.G.H. 1988; Characterization of the conjugation system associated with the Staphylococcus aureus plasmid pJEl. Journal of General Microbiology 134:1–8
    [Google Scholar]
  5. Fosgren A., Ghetite V., Lindmark R., Sj𝜙quist J. 1983; Protein A and its exploitation. In Staphylococci and Staphylococcal Infections, 2 pp 429–478 Easmon C. S. F., Adlam C. Edited by London: Academic Press;
    [Google Scholar]
  6. Gardiner K., Laas W., Patterson D. 1986; Fractionation of large mammalian DNA restriction fragments using vertical pulsed-field gradient gel electrophoresis. Somatic Cell and Molecular Genetics 12:185–195
    [Google Scholar]
  7. Guss B., Uhlén M., Nilsson B., Lindberg M., Sj𝜙quist J., Sj𝜙pdahl J. 1984; Region X, the cell- wall-attachment part of staphylococcal protein A. European Journal of Biochemistry 138:413–420
    [Google Scholar]
  8. Kaida S., Miyata T., Yoshizawa Y., Kawabata S., Morita T., Igarashi H., Iwanaga S. 1987; Nucleotide sequence of the staphylocoagulase gene: its unique COOH-terminal 8 tandem repeats. Journal of Biochemistry 102:1177–1186
    [Google Scholar]
  9. Kuhl S.A., Pattee P.A., Baldwin J.N. 1978; Chromosomal map location of the methicillin resistance determinant in Staphylococcus aureus.. Journal of Bacteriology 135:460–465
    [Google Scholar]
  10. Langone J.J. 1982; Protein A of Staphylococcus aureus and related immunoglobulin receptors produced by streptococci and pneumococci. Advances in Immunology 32:157–252
    [Google Scholar]
  11. Lee C.Y., Iandolo J.J. 1986; Lysogenic conversion of staphylococcal lipase is caused by insertion of the bacteriophage L54a genome into the lipase structural gene. Journal of Bacteriology 166:385–391
    [Google Scholar]
  12. L𝜙fdahl S., Guss B., Uhlèn M., Philipson L., Lindberg M. 1983; Gene for staphylococcal protein A. Proceedings of the National Academy of Sciences of the United States of America 80:697–701
    [Google Scholar]
  13. Luchansky J.B., Pattee P.A. 1984; Isolation of transposon Tn551 insertions near chromosomal markers of interest in Staphylococcus aureus.. Journal of Bacteriology 159:894–899
    [Google Scholar]
  14. Mallonee D.H., Glatz B.A., Pattee P.A. 1982; Chromosomal mapping of a gene affecting enterotoxin A production in Staphylococcus aureus.. Applied and Environmental Microbiology 43:397–402
    [Google Scholar]
  15. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;
    [Google Scholar]
  16. Novick R.P. 1963; Properties of a cryptic high- frequency transducing phage in Staphylococcus aureus.. Virology 33:155–166
    [Google Scholar]
  17. O’Reilly M., DeAzavedo J.C.S., Kennedy S., Foster T.J. 1986; Inactivation of the alpha- haemolysin of Staphylococcus aureus 8325-4 by site- directed mutagenesis and studies on expression of its haemolysins. Microbial Pathogenesis 1:125–138
    [Google Scholar]
  18. Patel A.H., Nowlan P., Weavers E.D., Foster T.J. 1987; Virulence of protein A-deficient and alpha-toxin-deficient mutants of Staphylococcus aureus isolated by allele-replacement. Infection and Immunity 55:3103–3110
    [Google Scholar]
  19. Pattee P.A. 1981; Distribution of Tn551 insertion sites responsible for auxotrophy on the Staphylococcus aureus chromosome. Journal of Bacteriology 145:479–488
    [Google Scholar]
  20. Pattee P.A. 1986; Chromosome map location of the alpha-hemolysin structural gene in Staphylococcus aureus NCTC 8325. Infection and Immunity 54:593596
    [Google Scholar]
  21. Pattee P.A. 1987 Staphylococcus aureus. Genetic Maps 4:148–154
    [Google Scholar]
  22. Pattee P.A., Glatz B.A. 1980; Identification of a chromosomal determinant of enterotoxin A production in Staphylococcus aureus.. Applied and Environmental Microbiology 39:186–193
    [Google Scholar]
  23. Pattee P.A., Neveln D.S. 1975; Transformation analysis of three linkage groups in Staphylococcus aureus.. Journal of Bacteriology 124:201–211
    [Google Scholar]
  24. Peterson P.K., Verhoef J., Sabath L.D., Quiie P.G. 1977; Effect of protein A on staphylococcal opsonization. Infection and Immunity 15:760–764
    [Google Scholar]
  25. Recsei P., Kreiswirth B., O’Reilly M., Schlie-Vert P., Gruss A., Novick R.P. 1986; Regulation of exoprotein gene expression in Staphylococcus aureus by agr.. Molecular and General Genetics 202:58–61
    [Google Scholar]
  26. Rigby P.W.J., Diekman M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  27. Schroeder C.J., Pattee P.A. 1984; Transduction analysis of transposon Tn551 insertions in the trp-thy region of the Staphylococcus aureus chromosome. Journal of Bacteriology 157:533–537
    [Google Scholar]
  28. Smith C.L., Warburton P.E., Gaal A., Cantor C.R. 1986; Analysis of genome organization and rearrangement by pulsed-field gradient gel electrophoresis. In Genetic Engineering, Principles and Methods, pp. 45–70 Setlow J.K., Hollaender A. New York:: Plenum Press.;
    [Google Scholar]
  29. Southern E.M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  30. Stahl M.L., Pattee P.A. 1983a; Computer- assisted chromosome mapping by protoplast fusion in Staphylococcus aureus.. Journal of Bacteriology 154:395–405
    [Google Scholar]
  31. Stahl M.L., Pattee P.A. 1983b; Confirmation of protoplast fusion-derived linkages in Staphylococcus aureus by transformation with protoplast DNA. Journal of Bacteriology 154:406–412
    [Google Scholar]
  32. Trees D.L., Iandolo J.J. 1988; Identification of a Staphylococcus aureus transposon (Tn4291) that carries the methicillin resistance gene(s). Journal of Bacteriology 170:149–154
    [Google Scholar]
  33. Uhlén M., Guss B., Nilsson B., Gatenbeck S., Philipson L., Lindberg M. 1984; Complete sequence of the staphylococcal gene encoding protein A. A gene evolved through multiple duplications. Journal of Biological Chemistry 259:1695–1702
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-7-1799
Loading
/content/journal/micro/10.1099/00221287-135-7-1799
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error