SUMMARY: The gene encoding lipoamide dehydrogenase (dihydrolipoamide dehydrogenase; EC was isolated from a library of DNA cloned in TG2 by use of serum raised against lipoamide dehydrogenase from Large amounts (up to 15% of total cellular protein) of the lipoamide dehydrogenase were produced by the clone harbouring plasmid pCJB94 with the lipoamide dehydrogenase gene. The enzyme was purified to homogeneity by a three-step procedure. The gene was subcloned from plasmid pCJB94 and the complete nucleotide sequence of the subcloned fragment (3610 bp) was determined. The derived amino acid sequence of lipoamide dehydrogenase showed 84% and 42% homology when compared to the amino acid sequences of lipoamide dehydrogenase from and , respectively. The gene of . is clustered in the genome with genes for the other components of the 2-oxoglutarate dehydrogenase complex.


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