1887

Abstract

The gene encoding lipoamide dehydrogenase (dihydrolipoamide dehydrogenase; EC 1.8.1.4) was isolated from a library of DNA cloned in TG2 by use of serum raised against lipoamide dehydrogenase from Large amounts (up to 15% of total cellular protein) of the lipoamide dehydrogenase were produced by the clone harbouring plasmid pCJB94 with the lipoamide dehydrogenase gene. The enzyme was purified to homogeneity by a three-step procedure. The gene was subcloned from plasmid pCJB94 and the complete nucleotide sequence of the subcloned fragment (3610 bp) was determined. The derived amino acid sequence of lipoamide dehydrogenase showed 84% and 42% homology when compared to the amino acid sequences of lipoamide dehydrogenase from and , respectively. The gene of . is clustered in the genome with genes for the other components of the 2-oxoglutarate dehydrogenase complex.

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1989-07-01
2024-12-07
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References

  1. Birnboim H.C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  2. Browning K.S., Uhlinger D,. J., Reed L.J. 1988; Nucleotide sequence for yeast dihydrolipextraction amide dehydrogenase. Proceedings of the National Academy of Sciences of the United States of America 85:1831–1834
    [Google Scholar]
  3. Dickinson J.R., Roy D.J., Dawes J.W. 1986; A mutation affecting lipoamide dehydrogenase, pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase activities in Saccharomyces cerevisiae. Molecular and General Genetics 204:103–107
    [Google Scholar]
  4. Fox B.S., Walsh C.T. 1982; Mercuric reductase - purification and characterization of transposon- encoded flavoprotein containing an oxidation- reduction-active disulfide. Journal of Biological Chemistry 257:2498–2503
    [Google Scholar]
  5. Gibson T.J. 1984 Studies on the Epstein-Bar virus genome. PhD thesis University of Cambridge; UK:
    [Google Scholar]
  6. Guest J.R., Cole S.T., Jeyaseelan K. 1981; Organization and expression of the pyruvate dehydrogenase complex genes of Escherichia coli K12. Journal of General Microbiology 127:65–79
    [Google Scholar]
  7. Hanemaaijer R., Janssen A., De kok A., Veeger C. 1988; The dihydrolipoyltransacetylase component of the pyruvate dehydrogenase complex of Azotobacter vinelandii. European Journal of Biochemistry 174:593–599
    [Google Scholar]
  8. Holmgren A. 1980; Pyridine nucleotide-disulfide oxidoreductases. Experientia, Supplement 36:149–180
    [Google Scholar]
  9. Howell L.G., Spector T., Massey V. 1972; Purification and properties of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens. Journal of Biological Chemistry 247:4340–4350
    [Google Scholar]
  10. Langley P., Guest J.R. 1977; Biochemical genetics of the a-ketoacid dehydrogenase complexes of Escherichia coli K12: isolation and biochemical properties of deletion mutants. Journal of General Microbiology 99:263–276
    [Google Scholar]
  11. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  12. Mccully V., Burns G., Sokatch J.R. 1986; Resolution of branched chain oxoacid dehydrogenase complex of Pseudomonas aeruginosa PAO. Biochemical Journal 233:737–742
    [Google Scholar]
  13. Norrander J., Kempe T., Messing J. 1983; Construction of improved Ml3 vectors using oligo- deoxynucleotide-directed mutagenesis. Gene 26:101–106
    [Google Scholar]
  14. Otulakowski G., Robinson B.H. 1987; Isolation and sequence determination of cDNA clones for porcine and human lipoamide dehydrogenases. Journal of Biological Chemistry 262:17313–17318
    [Google Scholar]
  15. Otulakowski G., Robinson B.H., Willard H.F. 1988; Gene for lipoamide dehydrogenase maps to human chromosome 7. Somatic Cell and Molecular Genetics 14:411–414
    [Google Scholar]
  16. Rice D.W., Schulz G.E., Guest J.R. 1984; Structural relationships between glutathione reductase and lipoamide dehydrogenase. Journal of Molecular Biology 174:483–496
    [Google Scholar]
  17. Rigby P.W.J., Dieckmann M., Rhodes C., Berg P. 1977; Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  18. Ross J., Reid G.A., Dawes J.W. 1988; The nucleotide sequence of the LPD1 gene encoding lipoamide dehydrogenase in Saccharomyces cerevisiae : comparison between eukaryotic and prokaryotic sequences for related enzymes and identification of potential upstream control sites. Journal of General Microbiology 134:1131–1139
    [Google Scholar]
  19. Sanger F., Nicklen S., Coulson A.R. 1977; DNA sequencing with chain terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 74:5463–5467
    [Google Scholar]
  20. Schierbeek A.J., Swarte B.M.A., Dljkstra B.W., Vriend G., Read R.J., Hol W.J.G., Drenth J., Betzel C. 1989; Structure of lipoamide dehydrogenase from Azotobacter vinelandii determined by a combination of molecular and isomorphous replacement techniques. Journal of Molecular Biology 206:365–379
    [Google Scholar]
  21. Scouten W.H., Mcmanus I.R. 1971; Microbial lipoamide dehydrogenase - purification and some characteristics of the enzyme derived from selected microorganisms. Biochimica et biophysica acta 227:248–263
    [Google Scholar]
  22. Shames S.L., Fairlamb A.H., Cerami A., Walsh C.T. 1986; Purification and characterization of trypanothione reductase from Chrithidia fasciculata, a newly discovered member of disulfide containing flavoprotein reductase. Biochemistry 25:3519–3526
    [Google Scholar]
  23. Sokatch J.R., Burns G. 1984; Oxidation of glycin by Pseudomonas putida requires a specific lipoamide dehydrogenase. Archives of Biochemistry and Biophysics 228:660–666
    [Google Scholar]
  24. Sokatch J.R., Mccully V., Gebroski J., Sokatch D.J. 1981; Isolation of a specific dehydrogenase for a branched chain ketoacid dehydrogenase from Pseudomonas putida. Journal of Bacteriology 148:639–646
    [Google Scholar]
  25. Southern E.M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  26. Spencer M.E., Darlison M.G., Stephens P.E., Duckenfield J.K., Guest J.R. 1984; Nucleotide sequence of the sucB gene encoding the dihydrolipoyl succinyl transferase of Escherichia coli K12 and homology with the corresponding acetyl transferase. European Journal of Biochemistry 141:361–374
    [Google Scholar]
  27. Staden R. 1982; Automation of the computer handling of gelreading data produced by the shotgun method of DNA sequencing. Nucleic Acids Research 10:4731–4751
    [Google Scholar]
  28. Staden R. 1984; A computer program to enter DNA gelreading data into a computer. Nucleic Acids Research 12:499–503
    [Google Scholar]
  29. Stephens P.E., Lewis H.M., Darlison M.G., Guest J.R. 1983; Nucleotide sequence of the lipoamide dehydrogenase gene of Escherichia coli K12. European Journal of Biochemistry 135:519–527
    [Google Scholar]
  30. Sykes P.J., Menard J., Mccully V., Sokatch J.R. 1985; Conjugative mapping of pyruvate, 2-ketoglutarate and branched chain ketoacid dehydrogenase genes in Pseudomonas putida mutants. Journal of Bacteriology 162:203–208
    [Google Scholar]
  31. Tinoco I.JR Borer P.N., Dengler B., Levine M.D., Uhlenbeck O.C., Crothers D.M., Gralla J. 1973; Improved estimation of secondary structure in ribonucleic acids. Nature New Biology 246:40–41
    [Google Scholar]
  32. Van Berkel W.J.H., Van den berg W.A.M., Müller F. 1988; Large scale preparation and reconstitution of apo-flavoproteins with special reference to butyryl-CoA dehydrogenase from Megasphaera elsdenii: hydrophobic interaction chromatography. European Journal of Biochemistry 178:197–207
    [Google Scholar]
  33. Van den broek H.W.J. 1971 Pyridine nucleotide transhydrogenase. PhD thesis Agricultural University of Wageningen; The Netherlands:
    [Google Scholar]
  34. Vieira J., Messing J. 1982; The pUC plasmids, an M13mp7 derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
    [Google Scholar]
  35. Westphal A.H., De kok A. 1988; Lipoamide dehydrogenase from Azotobacter vinelandii; molecular cloning, organization and sequence analysis of the gene. European Journal of Biochemistry 172:299–305
    [Google Scholar]
  36. Williams C.H.JR 1976; Flavin containing dehydrogenases. In The Enzymes 13 pp. Boyer P.D. Edited by New York: Academic Press;
    [Google Scholar]
  37. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved Ml3 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
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