cDNA clones representing eight specific mRNAs abundantly expressed in a dikaryon of but not in the progenitor monokaryons, were used to localize these mRNAs in whole colonies by hybridization. A genomic clone for 18S rRNA was used to probe for rRNA. The colonies were grown on hybridization membranes, fixed, and treated with RNAase-depleted wall-lytic enzymes from to facilitate permeation of the probes. RNAs in sectors of the colonies were then localized with [P]DNA probes. Hybridization at different developmental stages showed that the specific mRNAs were only formed in parts of the colony where fruit-body initials arose. At later stages these mRNAs disappeared from abortive fruit bodies but remained high in fruit bodies continuing development.


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