1887

Abstract

cDNA clones representing eight specific mRNAs abundantly expressed in a dikaryon of but not in the progenitor monokaryons, were used to localize these mRNAs in whole colonies by hybridization. A genomic clone for 18S rRNA was used to probe for rRNA. The colonies were grown on hybridization membranes, fixed, and treated with RNAase-depleted wall-lytic enzymes from to facilitate permeation of the probes. RNAs in sectors of the colonies were then localized with [P]DNA probes. Hybridization at different developmental stages showed that the specific mRNAs were only formed in parts of the colony where fruit-body initials arose. At later stages these mRNAs disappeared from abortive fruit bodies but remained high in fruit bodies continuing development.

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1989-06-01
2024-12-04
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References

  1. Akam M.E. 1983; The location of ultrabithorax transcripts inDrosophila tissue sections.. EMBO Journal 2:2075–2084
    [Google Scholar]
  2. Blanc H., Dujon J.B., Guerineau M., Slon-Imski P.P. 1978; Detection of specific DNA sequences in yeast by colony hybridization.. Molecular and General Genetics 161:311–315
    [Google Scholar]
  3. Cornish E.C., Pettitt J.M., Bonig I., Clarke A.E. 1987; Developmentally controlled expression of a gene associated with self-incompatibility inNicotiana alata.. Nature London: 326:99–102
    [Google Scholar]
  4. Cox K.H., DeLeon D.V., Angerer L.M., Angerer R.C. 1984; Detection of mRNAs in sea urchin embryos byin situ hybridization using asymmetric RNA probes.. Developmental Biology 101:485–502
    [Google Scholar]
  5. Crowley P., Oliver S.G. 1987; A microculture hybridization technique for the detection of specific DNA sequences in filamentous fungi.. Experimental Mycology 11:70–73
    [Google Scholar]
  6. DeVries O.M.H., Wessels J.G.H. 1972; Release of protoplasts fromSchizophyllum communeby a lytic enzyme preparation from Trichoderma viride.. Journal of General Microbiology 73:13–22
    [Google Scholar]
  7. DeVries O.M.H., Wessels J.G.H. 1973; Effectiveness of a lytic enzyme preparation fromTrichoderma viride in releasing spheroplasts from fungi, particularly basidiomycetes.. Antonie van Leeuwenhoek 39:397–400
    [Google Scholar]
  8. Hafen E., Levine M., Garber R.L., Gehring W.J. 1983; An improvedin situ hybridization method for the detection of cellular RNAs in Drosophila tissue sections and its application for localizing transcripts of the antennapedia gene complex.. EMBO Journal 2:617–623
    [Google Scholar]
  9. Hinnen A., Hicks J.B., Fink G.R. 1978; Transformation of yeast.. Proceedings of the National Academy of Sciences of the United States of America 75:1929–1933
    [Google Scholar]
  10. Martineau B., Taylor W.C. 1986; Cell-specific photosynthetic gene expression in maize determined using cell separation techniques and hybridizationin situ.. Physiologia plantarum 82:613–618
    [Google Scholar]
  11. Meinkoth J., Wahl G. 1984; Hybridization of nucleic acids bound to solid supports.. Analytical Biochemistry 138:267–284
    [Google Scholar]
  12. Mulder G.H., Wessels J.G.H. 1986; Molecular cloning of RNAs differentially expressed in mono- karyons and dikaryons ofSchizophyllum commune in relation to fruiting.. Experimental Mycology 10:214–227
    [Google Scholar]
  13. Raper J. R., Hoffman R. M. 1974 Schizophyllum commune. In Handbook of Genetics 1:pp.597–626 King R. C. New York:: Plenum.;
    [Google Scholar]
  14. Ruiters M. H. J., Wessels J. G. H. 1989; In situ localization of specific mRNAs in developing fruit bodies of the basidiomycete Schizophyllum commune.. Experimental Mycology 13: (in the Press).
    [Google Scholar]
  15. Ruiters M.H.J., Sietsma J.H., Wessels J.G.H. 1988; Expression of dikaryon-specific mRNAs ofSchizophyllum commune in relation to incompatibility genes, light, and fruiting.. Experimental Mycology 12:60–69
    [Google Scholar]
  16. Schmelzer E., Jahnen W., Hahlbrock K. 1988; In situ localization of light-induced chalcone synthetase mRNA, chalcone synthase, and flavonoid end products in epidermal cells of parsley leaves.. Proceedings of the National Academy of Sciences of the United States of America 85:2989–2993
    [Google Scholar]
  17. Smith A.G., Hinchee M., Horsch R. 1987; Cell and tissue specific expression localized byin situRNA hybridization in floral tissues.. Plant Molecular Biology Reporter 5:237–241
    [Google Scholar]
  18. Sonnenberg A.S.M., Wessels J.G.H. 1987; Heterokaryon formation in the basidiomyceteSchizophyllum commune by electrofusion of protoplasts.. Theoretical and Applied Genetics 74:654–658
    [Google Scholar]
  19. Stohl L.L., Lambowitz A.M. 1983; A colony- filter-hybridization procedure for the filamentous fungusNeurospora crassa.. Analytical Biochemistry 134:82–85
    [Google Scholar]
  20. Wessels J.G.H. 1965; Morphogenesis and biochemical processes inSchizophyllum commune.. Wen-tia 13:1–113
    [Google Scholar]
  21. Wessels J.G.H. 1987; Mating-type genes and the control of expression of fruiting genes in basidiomy- cetes.. Antonie van Leeuwenhoek 53:307–317
    [Google Scholar]
  22. Wessels J.G.H., Sietsma J.H. 1979; Wall structure and growth inSchizophyllum commune.. In Fungal Walls and Hyphal Growth (British Mycological Society Symposium 2), pp 27–48 Burnett J. H., Trinci A. P. J. Edited by Cambridge: Cambridge University Press;
    [Google Scholar]
  23. Wessels J.G.H., Mulder G.H., Springer J. 1987; Expression of dikaryon-specific mRNAs ofSchizophyllum commune in relation to environmental conditions and fruiting.. Journal of General Microbiology 133:2557–2561
    [Google Scholar]
  24. Yli-Mattila T., Ruiters M.H.J., Wessels J.G.H. 1989; Photoregulation of dikaryon specific mRNAs and proteins by UV-A light inSchizophyllum commune.. Current Microbiology 19: (in the Press).
    [Google Scholar]
  25. Yu S.-M., Gorovsky M. A. 1986; In situ dotblots: quantitation of mRNA in intact cells.. Nucleic Acids Research 14:7597–7615
    [Google Scholar]
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