1887

Abstract

Eighteen sequences complementary to less-conserved regions within the 16S and 23S ribosomal ribonucleic acid (rRNA) of were subcloned or chemically synthesized and used as probes in a dot-spot deoxyribonucleic acid (DNA): DNA hybridization format. Some of these probes exclusively detected nucleic acid, whereas others also showed hybridization signals with nucleic acid from other bacterial species. Our results indicate that rRNA-derived DNA-probes can be used to differentiate between very closely related taxa without the use of Southern-blot analysis.

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/content/journal/micro/10.1099/00221287-135-6-1735
1989-06-01
2019-11-22
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-6-1735
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