RT Journal Article SR Electronic(1) A1 Jekkel, Antónia A1 Csajági, éva A1 Ilky, éva A1 Ambrus, GáborYR 1989 T1 Genetic Recombination by Spheroplast Fusion of Sterol-transforming Mycobacterium Strains JF Microbiology, VO 135 IS 6 SP 1727 OP 1733 DO https://doi.org/10.1099/00221287-135-6-1727 PB Microbiology Society, SN 1465-2080, AB Wall-deficient forms of fast-growing mycobacteria were produced in growth medium containing vancomycin and glycine, and spheroplasts were prepared by lysozyme treatment of wall-deficient cells. Spheroplasts gave rise to recombinants with high frequency (2-6%) when they were fused using polyethylene glycol 6000. The results demonstrated that in vivo genetic recombination could be used to produce genetically modified Mycobacterium strains with applications in transformation of steroids. Useful intermediates of steroid drug synthesis and new degradation products were obtained from sterols by selected recombinant strains., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-135-6-1727