1887

Abstract

The colicin A lysis protein, Cal, is synthesized at the same time as colicin A by harbouring plasmid pColA after induction by mitomycin C. Its function in the induced bacteria involves the release of colicin A, quasi-lysis, the death of the producing cells and the activation of the outer membrane phospholipase A. We have found that these various functions are affected differently by treatment of the induced cells with Triton X-100, divalent cations or EDTA. Triton X-100 and EDTA caused increased quasi-lysis and a higher level of mortality of the producing cells, but while Triton X-100 enhanced the release of colicin A, EDTA reduced it. Divalent cations protected the cells against both killing and quasi-lysis without greatly affecting colicin release. The effects of these agents were similar for both wild-type and phospholipase A mutants and depended only on the presence of a functional gene.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-6-1715
1989-06-01
2021-05-15
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/6/mic-135-6-1715.html?itemId=/content/journal/micro/10.1099/00221287-135-6-1715&mimeType=html&fmt=ahah

References

  1. Altieri M., Suit J.L., Fan M.L.J., Luria S.E. 1986; Expression of the cloned ColElkil gene in normal and kilR Escherichia coli.. Journal of Bacteriology 168:648–654
    [Google Scholar]
  2. Ames G.F. 1968; Lipids ofSalmonella typhimuriumand Escherichia coli: structure and metabolism. Journal of Bacteriology 95:833–843
    [Google Scholar]
  3. Baty D., Knibiehler M., Verheij H., Pattus F., Shire D., Bernadac A., Lazdunski C. 1987a; Site-directed mutagenesis of the COOH-terminal region of colicin A : effect on secretion and voltagedependent channel activity. Proceedings of the National Academy of Sciences of the United States of America 84:1152–1156
    [Google Scholar]
  4. Baty D., Lloubés R., Geli V., Lazdunski C., Howard S.P. 1987b; Extracellular release of colicin A is non-specific. EM BO Journal 6:2463–2468
    [Google Scholar]
  5. Cavard D., Lloubés R., Morlon J., Chartier M., Lazdunski C. 1985; Lysis protein encoded by plasmid ColA-CA31. Gene sequence and export. Molecular and General Genetics 199:95–100
    [Google Scholar]
  6. Cavard D., Crozel V., Gorvel J.P., Pattus F., Baty D., Lazdunski C. 1986; A molecular, genetic and immunological approach to the functioning of colicin A, a pore-forming protein. Journal of Molecular Biology 187:449–459
    [Google Scholar]
  7. Cavard D., Baty D., Howard S.P., Verheij H.M., Lazdunski C. 1987; Lipoprotein nature of the colicin A lysis protein: effect of amino acid substitutions at the site of modification and processing. Journal of Bacteriology 169:2187–2194
    [Google Scholar]
  8. Chakraborti A.S., Ishidate K., Cook W.R., Zrike J., Rothfield L.J. 1986; Accumulation of a murein-membrane attachment site fraction when cell division is blocked inIkyD and chamutants of Salmonella typhimurium and Escherichia coli.. Journal of Bacteriology 168:1422–1429
    [Google Scholar]
  9. DeGraaf F.K., Oudega B. 1986; Production and release of cloacin DF13 and related colicins. Current Topics in Microbiology and Immunology 125:183–205
    [Google Scholar]
  10. Howard S.P., Leduc M., Van Heijenoort J., Lazdunski C. 1987; Lysis and release of colicin A in colicinogenic autolytic deficientEscherichia colimutants. FEMS Microbiology Letters 42:147–151
    [Google Scholar]
  11. Howard S.P., Cavard D., Lazdunski C. 1989; Amino acid sequence and length requirements for assembly and function of the colicin A lysis protein. Journal of Bacteriology 171:410–418
    [Google Scholar]
  12. James R., Jarvis M., Barker D.F. 1987; Nucleotide sequence of the immunity and lysis region of the ColE9-J plasmid. Journal of General Microbiology 133:1553–1562
    [Google Scholar]
  13. Leduc M., Kasra R., VanHeijenoort J. 1982; Induction and control of the autolytic system ofEscherichia coli.. Journal of Bacteriology 152:26–34
    [Google Scholar]
  14. Leduc M., Kasra R., Singer H., VanHeijnoort J. 1984; EDTA induced autolysis inEscherichia coliand isolation of resistant mutants. FEMS Microbiology Letters 23:137–142
    [Google Scholar]
  15. Lloubés R., Baty D., Lazdunski C. 1986; The promoters of the genes for colicin production, release and immunity in the ColA plasmid: effects of convergent transcription and LexA protein. Nucleic Acids Research 14:2621–2636
    [Google Scholar]
  16. Luirink J., VanDerSande C., Tommassen J., Veltkamp E., DeGraaf F.K., Oudega B. 1986; Effects of divalent cations and of phospholipase A activity on excretion of cloacin DF13 and lysis of host cells. Journal of General Microbiology 132:825–834
    [Google Scholar]
  17. Luirink J., DeGraaf F.K., Oudega B. 1987; Uncoupling of synthesis and release of cloacin DF13 and its immunity protein byEscherichia coli.. Molecular and General Genetics 206:126–132
    [Google Scholar]
  18. Pugsley A.P. 1988; The immunity and lysis genes of ColN plasmid pCHAP4. Molecular and General Genetics 211:335–341
    [Google Scholar]
  19. Pugsley A.P., Schwartz M. 1983; A genetic approach to the study of mitomycin-induced lysis ofEscherichia coli K12 strains which produce colicin E2. Molecular and General Genetics 190:266–372
    [Google Scholar]
  20. Pugsley A.P., Schwartz M. 1984; Colicin E2 release: lysis, leakage or secretion? Possible role of a phospholipase. EMBO Journal 3:2393–2397
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-6-1715
Loading
/content/journal/micro/10.1099/00221287-135-6-1715
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error