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Volume 135, Issue 6

Ultrastructural Details of the Apparatus of Gliding Motility of (Myxobacterales) Free

Abstract

Cell was preparations of the myxobacterium were fractionated by ion-exchange chromatography. Highly ordered chain-like structures were found in certain fractions when negatively stained specimens were inspected in the transmission electron microscope. The chain-like structures, which we call ‘strands’, were built up of two structural components: (1) a ring with an outer diameter of 12 to 15 nm which was regularly stacked with a periodicity of 14 nm. and (2) an elongated component, measuring 11·0 × 2·8 nm. Each ring appeared to be linked to the next one by two elongated components. Their orientation towards the elongated components was somewhat variable, which suggests a certain flexibility and a potential for conformational changes of the ‘strands’. Three or more of the ‘strands’ appeared to form a superstructure, which is defined as ‘ribbon’, which might undergo conformational changes leading to the observed strinkage in the transverse direction of about 40%. We suggest that the structures described crease contraction waves in the surface of the myxobacterial cell, and that those waves drag the cell along. The organization of the apparatus of gliding motility in the cell surface could only very rarely be seen. An exceptionally well-preserved whole cell in a negatively stained specimen showed a surface pattern consisting of parallel ridges, indicated by dark stain lines and 40 to 50 nm wade running helically around the cell pole. At a higher magnification these parallel ridges showed a distinct, periodic substructure normal to their long axis and with a spacing of 14 nm. which corresponds exactly to the periodicity of the stacked rings within the ‘strand’.

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© Society for General Microbiology 1989
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1989-06-01
2025-04-21
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References

  1. Bisalputra T., Oakley B.R., Walker D.C., Shields C.M. 1975; Microtubular complexes in blue-green algae. Protoplasma 86:19–28
     [Google Scholar]
  2. Burchard R.P. 1981; Gliding motility of procaryotes: ultrastructure, physiology and genetics. Annual Review of Microbiology 35:497–529
     [Google Scholar]
  3. Burchard R.P. 1984; Gliding motility and taxes. In Myxobacteria: Development and Cell Interactions, pp 139–161 Rosenberg E. Edited by Berlin: Springer Verlag;
     [Google Scholar]
  4. Burchard A.C., Burchard R.P., Kloetzel J.A. 1977; Intracellular, periodic structures in the gliding bacterium Myxococcus xanthus.. Journal of Bacteriology 132:666–672
     [Google Scholar]
  5. Castenholz R.W. 1982; Motility and taxes. In The Biology of Cyanobacteria, pp 413–439 Carr N.G., Whitton B. A. Edited by Oxford: Blackwell Scientific Publications;
     [Google Scholar]
  6. Doetsch R.N., Hageage G.J. 1968; Motility in procaryotic organisms: problems, points of view, and perspectives. Biological Reviews 43:317–362
     [Google Scholar]
  7. Duxburry T., Humphrey B.A., Marshall K.C. 1980; Continuous observations of bacterial gliding motility in a dialysis microchamber: the effects of inhibitors. Archives of Microbiology 124:169–175
     [Google Scholar]
  8. Dworkin M., Keller K.H., Weisberg D. 1983; Experimental observations consistent with a surface tension model of gliding motility of Myxococcus xanthus.. Journal of Bacteriology 155:1367–1371
     [Google Scholar]
  9. Follett E.A.C., Webley D.M. 1965; An electron microscopic study of the cell surface of Cytophaga johnsonii and some observations on related organisms. Antonie van Leeuwenhoek 31:361–382
     [Google Scholar]
  10. Gräf W. 1965; Bewegungsorganellen bei Myxobak- terien. Archiv für Hygiene und Bakteriologie 149:518–526
     [Google Scholar]
  11. Halfen L.N. 1973; Gliding motility of Oscillatoria:ultrastructural and chemical characterization of the fibrillar layer. Journal of Phycology 9:248–253
     [Google Scholar]
  12. Halfen L.N., Castenholz R.W. 1971; Gliding motility in the blue-green alga Oscillatoria princeps.. Journal of Phycology 7:133–145
     [Google Scholar]
  13. Henrichsen J. 1972; Bacterial surface translocation: a survey and a classification. Bacteriological Reviews 36:478–503
     [Google Scholar]
  14. Hodgkin J., Kaiser D. 1977; Cell-to-cell stimulation of movement in nonmotile mutants of Myxococcus.. Proceedings of the National Academy of Sciences of the United States of America 74:2938–2942
     [Google Scholar]
  15. Hodgkin J., Kaiser D. 1979a; Genetics of gliding motility in Myxococcus xanthus (Myxobacterales): genes controlling movements of single cells. Molecular and General Genetics 171:167–176
     [Google Scholar]
  16. Hodgkin J., Kaiser D. 1979b; Genetics of gliding motility in Myxococcus xanthus (Myxobacterales): two gene systems control movement. Molecular and General Genetics 171:177–191
     [Google Scholar]
  17. Humphrey B.A., Dickson M.R., Marshall K.C. 1979; Physicochemical and in situ observations on the adhesion of gliding bacteria to surfaces. Archives of Microbiology 120:231–238
     [Google Scholar]
  18. Jahn E. 1924; Beitrage zur botanischen Protistologie. I. Die Polyangiden. Leipzig: Gebr. Borntraeger.
     [Google Scholar]
  19. Jarosch R. 1967; Studien zur Bewegungsmechanik der Bakterien und Spirochaten des Hochmoores. Østerreichische botanische Zeitschrift 114:256–306
     [Google Scholar]
  20. Kaiser D. 1979; Social gliding is correlated with the presence of pili in Myxococcus xanthus.. Proceedings of the National Academy of Sciences of the United States of Amercia 76:5952–5956
     [Google Scholar]
  21. Kaiser D., Manoil C., Dworkin M. 1979; Myxobacteria: cell interactions, genetics and development. Annual Review of Microbiology 33:595–639
     [Google Scholar]
  22. Keller K.H., Grady M., Dworkin M. 1983; Surface tension gradients: feasible model for gliding motility of Myxococcus xanthus.. Journal of Bacteriology 155:1358–1366
     [Google Scholar]
  23. Lapidus I.R., Berg H.C. 1982; Gliding motility of Cytophaga spec, strain U67. Journal of Bacteriology 151:383–398
     [Google Scholar]
  24. Lünsdorf H., Spiess E. 1986; A rapid method of preparing perforated supporting foils for the thin carbon films used in high resolution transmission electron microscopy. Journal of Microscopy 144:211–213
     [Google Scholar]
  25. MacRae T.H., McCurdy H.D. 1975; Ultrastructural studies of Chondromyces crocatus vegetative cells. Canadian Journal of Microbiology 21:1815–1826
     [Google Scholar]
  26. MacRae T.H., McCurdy H.D. 1976; Evidence for motility-related fimbriae in the gliding microorganism Myxococcus xanthus.. Canadian Journal of Microbiology 22:1589–1593
     [Google Scholar]
  27. Pate J.L. 1985; Gliding motility in Cytophaga.. Microbiological Sciences 2:289–295
     [Google Scholar]
  28. Pate J.L., Chang L.Y.E. 1979; Evidence that gliding motility in procaryotic cells is driven by rotary assemblies in the cell envelopes. Current Microbiology 2:59–64
     [Google Scholar]
  29. Pate J.L., Ordal E.J. 1967; The fine structure of Chondrococcus columnaris. III. The surface layers of Chondrococcus columnaris.. Journal of Cell Biology 35:37–51
     [Google Scholar]
  30. Reichenbach H. 1981; Taxonomy of the gliding bacteria. Annual Review of Microbiology 35:339–364
     [Google Scholar]
  31. Ridgeway H.F., Lewin R.A. 1973; Globlet- shaped subunits from the wall of a marine gliding microbe. Journal of General Microbiology 79:119–128
     [Google Scholar]
  32. Schmidt-Lorenz W. 1969; The fine structure of the swarm cells of myxobacteria. Journal of Applied Bacteriology 32:22–23
     [Google Scholar]
  33. Schmidt-Lorenz W., Kühlwein H. 1968; Intra-cellulare Bewegungsorganellen der Myxobakterien. Archiv für Mikrobiologie 60:95–98
     [Google Scholar]
  34. Schmidt-Lorenz W., Kühlwein H. 1969; Beitrage zur Kenntnis der Myxobakterienzelle. 2. Mitteilung. Oberflächenstrukturen der Schwarmzel- len. Archiv für Microbiologie 68:405–426
     [Google Scholar]
  35. Stackebrandt E. 1985; Phylogeny and phylogenetic classification of prokaryotes. In Evolution of Prokaryotes (FEMS Symposium 29), pp 309–334 Schleifer K. H., Stackebrandt E. Edited by London: Academic Press;
     [Google Scholar]
  36. Valentine R.C., Shapiro B.M., Stadtman E.R. 1968; Regulation of glutamine synthetase; XII. Electron microscopy of the enzyme from Escherichia coli.. Biochemistry 7:2143–2152
     [Google Scholar]
/content/journal/micro/10.1099/00221287-135-6-1633
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Ultrastructural Details of the Apparatus of Gliding Motility of Myxococcus fulvus (Myxobacterales)
Microbiology 135, 1633 (1989); https://doi.org/10.1099/00221287-135-6-1633
/content/journal/micro/10.1099/00221287-135-6-1633
/content/journal/micro/10.1099/00221287-135-6-1633
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