1887

Abstract

Starvation of a marine sp. S14 for carbon, nitrogen and phosphorus resulted in a fourfold increase in cell number during the first 6 h in the starvation regime. This initial cell division of non-growing cells was dependent on both DNA and peptidoglycan synthesis as deduced from inhibition experiments using nalidixic acid and ampicillin. Inhibition of protein synthesis by the addition of chloramphenicol led to the cessation of both cell division and DNA synthesis after 40–60 min in the starvation regime. Starvation also induced resistance against autolytic cell wall degradation. Resistance to ampicillin-induced murein degradation was most extensive in the portion of the cell wall that was synthesized after the onset of starvation and was dependent on protein synthesis. The amount of -alanine per unit dry weight increased twofold during 24 h of starvation and an increased resistance to lysis induced by sonication was observed during this period. It is suggested that the fourfold increase in cell number during the first six hours of starvation requires proteins synthesized and that new rounds of DNA replication may be initiated during non-growth subsequent to 40–60 min of starvation. While the rate of DNA synthesis during the initial 40–60 min was unaffected by the blockage of protein synthesis, the mechanism conferring autolysis resistance was effectively inhibited.

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1989-06-01
2024-12-08
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References

  1. Amy P. S., Pauling C., Morita R. Y. 1983; Starvation-survival processes of a marine Vibrio. Applied and Environmental Microbiology 45:17481752
    [Google Scholar]
  2. Baker R. M., Singleton C., Hood M. A. 1983; Effects of nutrient deprivation on Vibrio cholerae. Applied and Environmental Microbiology 46:930–940
    [Google Scholar]
  3. Donachie W. D., Robinson A. C. 1987; Cell division: Parameter values and the process. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology 2 pp. 1578–1593 Neidhart F. C. Edited by Washington D.C: American Society for Microbiology;
    [Google Scholar]
  4. Donachie W. D., Begg K. J., Sullivan N. 1984; Morphogenes of Escherichia coli. In Microbial Development pp. 27–62 Losick R., Shapiro L. Edited by Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  5. Goodell W., Tomasz A. 1980; Alteration of Escherichia coli murein during amino acid starvation. Journal of Bacteriology 14:1009–1016
    [Google Scholar]
  6. Kusser W., Ishiguro E. E. 1985; Involvement of the relA gene in the autolysis of Escherichia coliinduced by inhibitors of peptidoglycan biosynthesis. Journal of Bacteriology 164:861–865
    [Google Scholar]
  7. Lark K. G., Repko T., Hoffman E. J. 1963; The effect of amino acid deprivation on subsequent deoxyribonucleic acid replication. Biochimica et biophysica acta 76:9–24
    [Google Scholar]
  8. Malmcrona-Friberg K., Tunlid A., Mårdén P., Kjelleberg S., Odham G. 1986; Chemical changes in cell envelope and poly-β-hydroxybutyrate during short term starvation of a marine bacterial isolate. Archives of Microbiology 144:340–345
    [Google Scholar]
  9. Mårdén P., Nystrøm T., Kjelleberg S. 1987; Uptake of leucine by a marine gram-negative heterotrophic bacterium during exposure to starvation conditions. FEMS Microbiology Ecology 45:233–241
    [Google Scholar]
  10. Mårdén P., Tunlid A., Malmcrona-Friberg K., Odham G., Kjelleberg S. 1985; Physiological and morphological changes during short term starvation of marine bacterial isolates. Archives of Microbiology 142:326–332
    [Google Scholar]
  11. Mårdén P., Hermansson M., Kjelleberg S. 1988; Incorporation of tritiated thymidine by marine bacterial isolates when undergoing a starvation survival response. Archives of Microbiology 149:421–432
    [Google Scholar]
  12. Messer W. 1972; Initiation of deoxyribonucleic acid replication in Escherichia coli B/r: chronology of events and transcriptional control of initiation. Journal of Bacteriology 112:7–12
    [Google Scholar]
  13. Von Meyenburg K., Hansen F. G. 1987; Regulation of chromosome replication. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology 2 pp. 1555–1577 Neidhardt F. C. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  14. Morita R. Y. 1982; Starvation survival of hetero-trophs in the marine environment. In Advances in Microbial Ecology 6 pp. 171–198 Marshall K. C. Edited by New York: Plenum Press;
    [Google Scholar]
  15. Morita R. Y. 1985; Starvation and miniaturisation of heterotrophs, with special emphasis on maintenance of the starved viable state. In Bacteria in Their Natural Environments pp. 111–130 Fletcher M. M., Floodgate G. D. Edited by London: Academic Press;
    [Google Scholar]
  16. Novitsky J. A., Morita R. Y. 1977; Survival of psycrophilic marine Vibrio under long term nutrient starvation. Applied and Environmental Microbiology 33:635–641
    [Google Scholar]
  17. Nystrøm T., Kjelleberg S. 1987; The effect of cadmium on starved heterotrophic bacteria isolated from marine waters. FEMS Microbiology Ecology 45:143–153
    [Google Scholar]
  18. Nystrøm T., Mårdén P., Kjelleberg S. 1986; Relative changes in incorporation rates of leucine and methionine during starvation survival of two bacteria isolated from marine waters. FEMS Microbiology Ecology 38:285–292
    [Google Scholar]
  19. Nystrøm T., Albertson N., Kjelleberg S. 1988; Synthesis of membrane and periplasmic proteins during starvation of a marine Vibrio sp. Journal of General Microbiology 134:1645–1651
    [Google Scholar]
  20. Pierucci O., Helmstetter C. E. 1969; Chromosome replication, protein synthesis and cell division in Escherichia coli. Federation Proceedings 28:1755–1760
    [Google Scholar]
  21. Pritchard R. H., Barth P. T., Collins J. 1969; Control of DNA synthesis in bacteria. London: Symposia of the Society for General Microbiology 19263–297
    [Google Scholar]
  22. Ryter A., Hirota Y., Schwarz U. 1973; Process of cellular division in Escherichia coli: growth pattern of E. coli murein. Journal of Molecular Biology 78:185–195
    [Google Scholar]
  23. Schaechter M. 1961; Patterns of cellular control during unbalanced growth. Cold Spring Harbor, NY: Cold Spring Harbor Symposia on Quantitative Biology; 26: pp. 53–62
    [Google Scholar]
  24. Sompayrac L., Maaløe O. 1973; Autorepressor model for control of DNA replication. Nature; London: 241133–135
    [Google Scholar]
  25. Tuomanen E. 1986; Newly made enzymes determine ongoing cell wall synthesis and the antibacterial effects of cell wall synthesis inhibitors. Journal of Bacteriology 167:535–543
    [Google Scholar]
  26. Tuomanen E., Tomasz A. 1986; Induction of autolysis in nongrowing Escherichia coli. Journal of Bacteriology 167:1077–1080
    [Google Scholar]
  27. Tuomanen E., Markiewicz Z., Tomasz A. 1988; Autolysis-resistant peptidoglycan of anomalous composition in amino acid starved Escherichia coli. Journal of Bacteriology 170:1373–1376
    [Google Scholar]
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