RT Journal Article SR Electronic(1) A1 Blackall, Linda L. A1 Parlett, J. H. A1 Hayward, A. C. A1 Minnikin, D. E. A1 Greenfield, P. F. A1 Harbers, Anne E.YR 1989 T1 Nocardia pinensis sp. nov., an Actinomycete Found in Activated Sludge Foams in Australia JF Microbiology, VO 135 IS 6 SP 1547 OP 1558 DO https://doi.org/10.1099/00221287-135-6-1547 PB Microbiology Society, SN 1465-2080, AB A new nocardioform actinomycete was isolated by filament micromanipulation during the course of a study into foaming in activated sludge plants in Australia. It constitutes the second most prevalent foaming organism in Australia after Nocardia amarae. These two foaming organisms can be differentiated morphologically, biochemically and chemotaxonomically. The microscopic appearance of the filaments of the new taxon resembles a pine tree. The filaments are Gram-positive, non-acid-fast, non-motile, non-sheathed and about 0·5–1·0 μm in diameter. On a complex medium, the colonies are orange, opaque, macroscopically dry and friable, microscopically moist and shiny, with a pasty texture and an entire edge. The strains are positive for catalase, oxidase and urease and are oxidative in their metabolism of glucose. No strain could degrade hypoxanthine, xanthine, tyrosine, casein, gelatin or aesculin and none could grow with lysozyme. The strains contain peptidoglycan type Alγ, cell wall type IV, whole cell sugar pattern type A, phospholipid type PII, menaquinones ω-cyclo-MK-8 (H4), fatty acids comprising straight chain saturated and unsaturated acids and tuberculostearic acid, and mycolic acids with 58–64 carbons containing substantial amounts of unsaturated chains in the 2-position. The name Nocardia pinensis has been chosen for the new taxon because of the pine tree like appearance of the organism on microscopy. The type strain, UQM3063, is deposited at the University of Queensland, Department of Microbiology Culture Collection., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-135-6-1547