1887

Abstract

Pigment was produced by cells carrying recombinant plasmids pNIL100, pNIL200 and pNIL400 containing DNA from sp. cells containing pNIL100 or pNIL200 (with DNA inserts from sp. JL10 and sp. ATCC 21145 respectively) produced both blue and pink pigments, while cells containing pNIL400 (with a DNA insert from sp. ATCC 21145) produced only pink pigment. Colonies of (pNIL100) and (pNIL200) were dark blue, whereas (pNIL400) colonies were pink. No pigment was detected in transformants containing pNIL100, pNIL200 or pNIL400. Restriction endonuclease mapping indicated that the cloned DNA fragments were different. The pigment gene(s) in pNIL200 producing both the blue and pink pigments were contained within a 2·8 kb DNA fragment. The pigments produced by transformants containing pNIL200 were characterized by visible and UV spectroscopy. No similar pigments were detected in sp. ATCC 21145.

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1989-06-01
2021-05-14
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References

  1. Apajalahti J.H.A., Salkinoja-Salonen M.S. 1986; Degradation of polychlorinated phenols by Rhodococcus chlorophenolicus.. Applied Microbiology and Biotechnology 25:62–67
    [Google Scholar]
  2. Clarke L., Carbon J. 1976; A colony bank containing synthetic ColEl hybrid plasmids representative of the entire Escherichia coli genome. Cell 9:91–99
    [Google Scholar]
  3. Cohen S.N., Chang A.C.Y., Hsu L. 1972; Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proceedings of the National Academy of Sciences of the United States of America 69:2110–2114
    [Google Scholar]
  4. Dagert M., Ehrlich S.D. 1979; Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene 6:23–28
    [Google Scholar]
  5. Dauenhauer S., Hull R.A., Williams R.P. 1984; Cloning and expression in Escherichia coli of Serratia marcescens genes encoding prodigiosin biosynthesis. Journal of Bacteriology 158:1128–1132
    [Google Scholar]
  6. Gerber N.N., Lechevalier M.P. 1976; Prodiginine (prodigiosin-like) pigments from Strep- tomyces and other aerobic actinomycetes. Canadian Journal of Microbiology 22:658–667
    [Google Scholar]
  7. Goodfellow M., Alderson G. 1977; The actinomycete genus Rhodococcus : a home for the ‘rhodochrous’ complex. Journal of General Microbiology 100:99–122
    [Google Scholar]
  8. Goodfellow M., Cross T. 1984; Classification. In The Biology of the Actinomycetes pp 7–164 Goodfellow M., Mordarski M., Williams S. T. Edited by London: Academic Press;
    [Google Scholar]
  9. Hill R.T., Illing N., Kirby R., Woods D.R. 1988; Development of pLR591, a Streptomyces- Escherichia coli positive selection shuttle vector. FEMS Microbiology Letters 57:223–226
    [Google Scholar]
  10. Hopwood D.A., Bibb M.J., Chater K.F., Kieser T., Bruton C.J., Kieser H.M., Lydiate D.T., Smith C.P., Ward J.M. 1985 Genetic Manipulation of Streptomyces: a Laboratory Manual Norwich, UK: The John Innes Foundation;
    [Google Scholar]
  11. Ish-Horowicz D., Burke J.F. 1981; Rapid and efficient cosmid cloning. Nucleic Acids Research 9:2989–2998
    [Google Scholar]
  12. King P.V., Blakesley R.W. 1986; Optimizing DNA ligations for transformation. Focus (Gaithersburg, Maryland Bethesda Research Laboratories) 8:1–3
    [Google Scholar]
  13. Kirby R., Usdin K. 1985; The isolation and restriction mapping of a miniplasmid from the actinomycete Nocardia corallina.. FEMS Microbiology Letters 21:57–59
    [Google Scholar]
  14. Kurane R., Suzuki T., Takahara Y. 1979; Removal of phthalate esters by activated sludge inoculated with a strain of Nocardia erythropolis.. Agricultural and Biological Chemistry 43:421–427
    [Google Scholar]
  15. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  16. Mikami Y., Takahashi K., Fukushima K., Yazawa K., Arai T. 1987; A new pigment from Streptomyces lavendulae.. Journal of Antibiotics 40:385–387
    [Google Scholar]
  17. Peczynska-Czoch W., Mordarski M. 1984; Transformation of xenobiotics. In The Biology of the Actinomycetes pp 287–336 Goodfellow M., Mordarski M., Williams S. T. Edited by London: Academic Press;
    [Google Scholar]
  18. Raymond R.L. 1971; Hydroxy-phenyl-α-ketobutyric acids. United States Patents Office, Patent no. 3592846.
    [Google Scholar]
  19. Rudd B.A.M., Hopwood D.A. 1980; A pigmented mycelial antibiotic in Streptomyces coeli- color: control by a chromosomal gene cluster. Journal of General Microbiology 119:333–340
    [Google Scholar]
  20. Southern E.M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  21. Tsao S-W., Rudd B.A.M., He D.-G., Chang C.-J., Floss H.G. 1985; Identification of a red pigment from Streptomyces coelicolor A3(2) as a mixture of prodigiosin derivatives. Journal of Antibiotics 38:128–131
    [Google Scholar]
  22. Vieira J., Messing J. 1982; The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
    [Google Scholar]
  23. Williams R. P., Qadri S. M. H. 1980; The pigments of Serratia.. In The Genus Serratia pp. 31–75 Edited by von Graevinitz A., Rubin S. J. Boca Raton: CRC Press;
    [Google Scholar]
  24. Zabeau M., Stanley K. 1982; Enhanced expression of cro-β-galactosidase fusion proteins under the control of the Pr promoter of bacteriophage X.. EMBO Journal 1:1217–1224
    [Google Scholar]
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