1887

Abstract

A convenient and reliable method has been established that allows a quantitative determination of -diamino[H]pimelic acid-labelled murein precursors in 1 ml culture samples of . Prior to separation by reversed-phase high-pressure liquid chromatography the lipid-linked intermediates were hydrolysed to release the muropeptides. The accuracy for the measurement of UDP--acetylmuramylpentapeptide (UDP-MurNAc-pentapeptide) was ± 1·9% (), for undecaprenyl-P-P-MurNAc-pentapeptide (lipid I) ± 10% () and for undecaprenyl-P-P-(GlcNAc-1→4)MurNAc-pentapeptide (lipid II) ± 5% (). The ratio of UDP-MurNAc-pentapeptide:lipid I:lipid II was about 300:1:3 for MC4100. The relative cellular concentrations of all three precursor molecules were found not to vary throughout the cell cycle. It is concluded that elongation and division of the murein sacculus is not controlled by oscillations in the concentrations of these late murein precursors.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-6-1499
1989-06-01
2021-05-13
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/6/mic-135-6-1499.html?itemId=/content/journal/micro/10.1099/00221287-135-6-1499&mimeType=html&fmt=ahah

References

  1. Anderson J.S., Matsuhashi M., Haskin M.A., Strominger J.L. 1967; Biosynthesis of the peptidoglycan of bacterial cell walls. II. Phospholipid carriers in the reaction sequence. Journal of Biological Chemistry 242:3180–3190
    [Google Scholar]
  2. Bochner B.R., Ames B.N. 1982; Complete analysis of cellular nucleotides by two-dimensional thin-layer chromatography. Journal of Biological Chemistry 257:9759–9769
    [Google Scholar]
  3. Dai D., Ishiguro E.E. 1988; murH, a new genetic locus in Escherichia coli involved in cell wall peptidoglycan biosynthesis. Journal of Bacteriology 170:2197–2201
    [Google Scholar]
  4. Donachie W.D., Begg K.J., Sullivan N.F. 1984; Morphogenes of Escherichia coli. In Microbial Development pp. 27–62 Losick R., Shapiro L. Edited by Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  5. Figdor C.G., Olijhoek A.J.M., Klencke S., Nanninga N., Bont W.S. 1981; Isolation of small cells from exponentially growing culture of Escherichia coli by centrifugal elutriation. FEMS Microbiology Letters 10:349–352
    [Google Scholar]
  6. Glauner B., Höltje J.-V., Schwarz U. 1988; The composition of the murein of Escherichia coli. Journal of Biological Chemistry 263:10088–10095
    [Google Scholar]
  7. Helmstetter C.E., Cooper S., Pierucci O. 1968; On the bacterial life sequence. Cold Spring Harbor Symposia on Quantitative Biology 33:809–822
    [Google Scholar]
  8. Hoffmann B., Messer W., Schwarz U. 1972; Regulation of polar cap formation in the life cycle of Escherichia coli. Journal of Supramolecular Structure 1:29–37
    [Google Scholar]
  9. Höltje J.-V., Nanninga N. 1984; The intracellular concentration of cyclic adenosine 3′,5′-monophosphate is constant throughout the cell cycle of Escherichia coli. FEMS Microbiology Letters 22:189–192
    [Google Scholar]
  10. Höltje J.-V., Schwarz U. 1985; Biosynthesis and growth of the murein sacculus. In Molecular Cytology of Escherichia coli pp. 77–119 Nanninga N. Edited by London: Academic Press;
    [Google Scholar]
  11. Ishiguro E.E., Ramey W.D. 1978; Involvement of the relA gene product and feedback inhibition in the regulation of UDP-N-acetylmuramyl-peptide synthesis in Escherichia coli. Journal of Bacteriology 135:766–774
    [Google Scholar]
  12. Kraus W., Höltje J.-V. 1987; Two distinct transpeptidation reactions during murein synthesis in Escherichia coli. Journal of Bacteriology 169:3099–3103
    [Google Scholar]
  13. Kubitscheck H., Pai S.R. 1988; Variation in precursor pool size during the division cycle of Escherichia coli: further evidence for linear cell growth. Journal of Bacteriology 170:431–435
    [Google Scholar]
  14. Mengin-Lecreulx D., Flouret B., Van Heijenoort J. 1982; Cytoplasmic steps of peptidoglycan synthesis in Escherichia coli. Journal of Bacteriology 151:1109–1117
    [Google Scholar]
  15. Mengin-Lecreulx D., Flouret B., Van Heijenoort J. 1983; Pool levels of UDP-N-acetylglucos-amine and UDP-N-acetyl-glucosamine-enolpyru-vate in Escherichia coli and correlation with peptidoglycan synthesis. Journal of Bacteriology 154:1284–1290
    [Google Scholar]
  16. Nanninga N., Woldringh C.L. 1985; Cell growth, genome duplication, and cell division. In Molecular Cytology of Escherichia coli pp. 259–318 Nanninga N. Edited by London: Academic Press;
    [Google Scholar]
  17. Olijhoek A.J.M., Klencke S., Pas E., Nanninga N., Schwarz U. 1982; Volume growth, murein synthesis, and murein cross-linkage during the division cycle of Escherichia coli PA 3092. Journal of Bacteriology 152:1248–1254
    [Google Scholar]
  18. Park J.T. 1952; Uridine-5′-pyrophosphate derivatives. Journal of Biological Chemistry 194:877–904
    [Google Scholar]
  19. Ramey W.D., Ishiguro E.E. 1978; Site of inhibition of peptidoglycan biosynthesis during the stringent response in Escherichia coli. Journal of Bacteriology 135:71–77
    [Google Scholar]
  20. Rogers H.J., Perkins H.R., Ward J.B. 1980 Microbial Cell Walls and Membranes pp. 246–248 London:: Chapman and Hall.;
    [Google Scholar]
  21. Rothfield L., Romeo D. 1971; Role of lipids in the biosynthesis of the bacterial cell envelope. Bacteriological Reviews 35:14–38
    [Google Scholar]
  22. Schwarz U., Asmus A., Frank H. 1969; Autolytic enzymes and cell division of Escherichia coli. Journal of Molecular Biology 41:419–429
    [Google Scholar]
  23. Spratt B.G. 1975; Distinct penicillin-binding proteins involved in the division, elongation, and shape of Escherichia coli K12. Proceedings of the National Academy of Sciences of the United States of America 72:2999–3003
    [Google Scholar]
  24. Stephens J.C., Artz S.W., Ames B.N. 1975; Guanosine 5′-diphosphate 3′-diphosphate (ppGpp): positive effector for histidine operon transcription and general signal for amino acid deficiency. Proceedings of the National Academy of Sciences of the United States of America 72:4389–4393
    [Google Scholar]
  25. Taschner P.E.M. 1988; Genetic and morphological analysis of cell division in Escherichia coli. Academisch Proefschrift, Universiteit van Amsterdam:
    [Google Scholar]
  26. Venkateswaran P.S., Lugtenberg E.J.J., Wu H.C. 1973; Inhibition of phosphoenolpyruvate: uridine diphosphate A-acetylglucosamine enol-pyruvyl-transferase by uridine diphosphate N-acetylmuramyl peptides. Biochimica et biophysica acta 293:570–574
    [Google Scholar]
  27. Vogel H.J., Bonner D.M. 1956; Acetylornithase of Escherichia coli, partial purification and some properties. Journal of Biological Chemistry 218:97–106
    [Google Scholar]
  28. Wientjes F.B., Olijhoek A.J.M., Schwarz U., Nanninga N. 1983; Labelling pattern of major penicillin-binding proteins of Escherichia coli during the division cycle. Journal of Bacteriology 153:1287–1293
    [Google Scholar]
  29. Wientjes F.B., Pas E., Taschner P.E.M., Woldringh C.L. 1985; Kinetics of uptake and incorporation of meso-diaminopimelic acid in different Escherichia coli strains. Journal of Bacteriology 164:331–337
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-6-1499
Loading
/content/journal/micro/10.1099/00221287-135-6-1499
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error