RT Journal Article SR Electronic(1) A1 Narbad, Arjan A1 Hewlins, Michael J. E. A1 Callely, A. G.YR 1989 T1 13C-NMR Studies of Acetate and Methanol Metabolism by Methylotrophic Pseudomonas Strains JF Microbiology, VO 135 IS 6 SP 1469 OP 1477 DO https://doi.org/10.1099/00221287-135-6-1469 PB Microbiology Society, SN 1465-2080, AB The metabolism of [2-13C]acetate by Pseudomonas M27(Icl−) and Pseudomonas MA(Icl+) was studied in vivo using 13C-NMR spectroscopy. The flux of 13C-label into bicarbonate, glutamate and citrate was observed in both organisms. In addition 13C-labelled α, α-trehalose was synthesized as a major metabolite by Pseudomonas M27 but not by Pseudomonas MA. The presence of this disaccharide in cell extracts of Pseudomonas AM1(Icl−) grown with [13C]methanol was also observed. The data from analysis of the trehalose multiplet signal observed in the spectra of Pseudomonas M27 cell extracts were consistent with the absence of the glyoxylate cycle in this methylotroph., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-135-6-1469