The metabolism of [2-C]acetate by M27(Icl) and MA(Icl) was studied using C-NMR spectroscopy. The flux of C-label into bicarbonate, glutamate and citrate was observed in both organisms. In addition C-labelled α,α-trehalose was synthesized as a major metabolite by M27 but not by MA. The presence of this disaccharide in cell extracts of AM1(Icl) grown with [C]methanol was also observed. The data from analysis of the trehalose multiplet signal observed in the spectra of M27 cell extracts were consistent with the absence of the glyoxylate cycle in this methylotroph.


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