1887

Abstract

The metabolism of [2-C]acetate by M27(Icl) and MA(Icl) was studied using C-NMR spectroscopy. The flux of C-label into bicarbonate, glutamate and citrate was observed in both organisms. In addition C-labelled α, α-trehalose was synthesized as a major metabolite by M27 but not by MA. The presence of this disaccharide in cell extracts of AM1(Icl) grown with [C]methanol was also observed. The data from analysis of the trehalose multiplet signal observed in the spectra of M27 cell extracts were consistent with the absence of the glyoxylate cycle in this methylotroph.

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1989-06-01
2022-01-24
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References

  1. Anthony C. 1982 The Biochemistry of Methylotrophs. London: Academic Press;
    [Google Scholar]
  2. Barton J.K., Den Hollander J.A., Hopfield J.J., Shulman R.G. 1982; 13C Nuclear magnetic resonance study of trehalose mobilization in yeast spores. Journal of Bacteriology 151:177–185
    [Google Scholar]
  3. Burton G., Baxter R.L., Gunn J.M., Sidebottom P.J., Fagerness P.E., Lee J.Y., Scott I. 1980; Direct non-invasive observation of metabolism in living cells by 13C nuclear magnetic resonance spectroscopy. Canadian Journal of Chemistry 58:1839–1846
    [Google Scholar]
  4. Cornish A., Nicholls K.M., Scott D., Hunter B.K., Aston W.J., Higgins I.J., Sanders J.K.M. 1984; In vivo 13C NMR investigation of methanol oxidation by the obligate methanotroph Methylosinus trichosporium OB3b. Journal of General Microbiology 130:2565–2575
    [Google Scholar]
  5. Dickinson J.R., Dawes I.W., Boyd A.S.F., Baxter R.L. 1983; 13C NMR studies of acetate metabolism during sporulation of Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences of the United States of America 80:5847–5851
    [Google Scholar]
  6. Dunstan P.M., Anthony C., Drabble W.T. 1972; Microbial metabolism of C1 and C2 compounds: involvement of glycollate in the metabolism of ethanol and acetate by Pseudomonas AM1. Biochemical Journal 128:99–106
    [Google Scholar]
  7. Elbein A.D. 1974; The metabolism of a,a-trehalose. Advances in Carbohydrate Chemistry and Biochemistry 30:227–256
    [Google Scholar]
  8. Den Hollander J.A., Brown T.R., Ugurbil K., Shulman R.G. 1979; 13C nuclear magnetic resonance studies of anaerobic glycolysis in suspensions of yeast cells. Proceedings of the National Academy of Sciences of the United States of America 76:6096–6100
    [Google Scholar]
  9. Den Hollander J.A., Ugurbil K., Brown T.R., Bednar M., Redfield C., Shulman R.G. 1986; Studies of anaerobic and aerobic glycolysis in Saccharomyces cerevisiae. Biochemistry 25:203–211
    [Google Scholar]
  10. Hou C.T. 1984 Methylotrophs: Microbiology, Biochemistry and Genetics. Boca Raton, Fla: CRC Press;
    [Google Scholar]
  11. Hunter B.K., Nicholls K.M., Sanders J.K.M. 1984; Formaldehyde metabolism by Escherichia coli. In vivo carbon, deuterium, and two-dimensional NMR observations of multiple detoxifying pathways. Biochemistry 23:508–514
    [Google Scholar]
  12. Kornberg H.L. 1966; Anaplerotic sequences and their role in metabolism. Essays in Biochemistry 2:1–31
    [Google Scholar]
  13. Kortstee G.J.J. 1980; The homoisocitrate-glyoxylate cycle in pink, facultative methylotrophs. FEMS Microbiology Letters 8:59–65
    [Google Scholar]
  14. Large P.J., Peel D., Quayle J.R. 1961; Microbial growth on C1 compounds. 2. Synthesis of cell constituents by methanol and formate grown Pseudomonas AM1 and methanol grown Hypho-microbium vulgare. Biochemical Journal 81:470–480
    [Google Scholar]
  15. Larsen P.I., Sydnes L.K., Landfald B., Strom A.R. 1987; Osmoregulation in Escherichia coli by accumulation of organic osmolytes: betains, glutAM1c acid and trehalose. Archives of Microbiology 147:1–7
    [Google Scholar]
  16. London R.E. 1988; 13C Labelling in studies of metabolic regulation. Progress in NMR Spectroscopy 20:337–383
    [Google Scholar]
  17. Mackay M.A., Norton R.S., Borowitzka L.J. 1984; Organic osmoregulatory solutes in cyanobacteria. Journal of General Microbiology 130:2177–2191
    [Google Scholar]
  18. Panek A.D. 1963; Function of trehalose in baker's yeast Saccharomyces cerevisiae. Archives of Biochemistry and Biophysics 100:422–425
    [Google Scholar]
  19. Reed R.H., Borowitzka L.J., Mackay M.A., Chudek J.A., Forster R., Warr S.R.C., Moore D.J., Stewart W.D.P. 1986; Organic solute accumulation in osmotically stressed cyanobacteria. FEMS Microbiology Reviews 39:51–56
    [Google Scholar]
  20. Salem A.R., Large P.J., Quayle J.R. 1972; Glycine formation during growth of Pseudomonas AM1 on methanol and succinate. Biochemical Journal 128:1203–1211
    [Google Scholar]
  21. Shulman R.G., Brown T.R., Ugurbil K., Ogawa S., Cohen S.M., Denhollander J.A. 1979; Cellular applications of 31P and 13C nuclear magnetic resonance. Science 205:160–166
    [Google Scholar]
  22. Thevelein J.M. 1984; Regulations of trehalose mobilisation in fungi. Microbiological Reviews 48:42–59
    [Google Scholar]
  23. Ugurbil K., Brown T.R., Den Hollander J.A., Glynn P., Shulman R.G. 1978; High-resolution 13C nuclear magnetic resonance studies of glucose metabolism in Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 75:3742–3746
    [Google Scholar]
  24. Walker T.E., Han C.H., Kollman V.H., London R.E., Matwiyoff N.A. 1982; 13C nuclear magnetic resonance studies of the biosynthesis by Microbacterium ammoniaphilum of L-glutamate selectively enriched with carbon-13. Journal of Biological Chemistry 257:1189–1195
    [Google Scholar]
  25. Walker T.E., London R.E. 1987; Biosynthetic preparation of L-[13C]-and [15N]glutamate by Brevibacterium flavum. Applied and Environmental Microbiology 53:92–98
    [Google Scholar]
  26. Walsh K., Koshland D.E. 1984; Determination of flux through the branch point of two metabolic cycles; the tricarboxylic acid cycle and the glyoxylate shunt. Journal of Biological Chemistry 259:9646–9654
    [Google Scholar]
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