Characterization of Methylammonium/Ammonium Transport in Mutant Strains of Resistant to Ammonium Analogues Free

Abstract

A methylamine-resistant mutant strain (4m3) of ATCC 29413 was capable of growth at pH 7·0, but not at pH 9·0, in the presence of concentrations of methylamine that totally inhibited growth of the parent strain. Strain 4m3 showed a marked reduction, at pH 7·0 but not at pH 9·0, in the initial rapid and slower second phases of uptake of [C]methylamine and ammonia, when compared with the parent strain, The rate of inhibition of nitrogenase activity, on adding ammonia at pH 7·0, was lower in strain 4m3 than in the parent strain and was attributable to a defect in the CHNH /NH transport system. Evidence that the second slower phase of [C]methylamine uptake is associated with metabolism via glutamine synthetase (GS) was obtained using mutant strains partially deficient in GS. Unlike these GS-deficient strains, strain 4m3 did not liberate ammonia extracellularly unless treated with -methionine-DL-sulphoximine. Growth of strain 4m3 in medium containing 3 m-methylamine resulted in a restoration of the first phase of [C]methylamine uptake, at pH 7·0, but not of ammonia uptake. However, growth in the presence of 3 m-ammonia did not enhance the uptake of either methylamine or ammonia. Strain 4m3 is apparently deficient in the active CHNH /NH transport system, but has a CHNH transport system which can be induced by growth in the presence of methylamine.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-6-1441
1989-06-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/6/mic-135-6-1441.html?itemId=/content/journal/micro/10.1099/00221287-135-6-1441&mimeType=html&fmt=ahah

References

  1. Alef K., Kleiner D. 1982; Evidence for an ammonium transport system in the N 2-fixing phototrophic bacterium Rhodospirillum rubrum.. Archives of Microbiology 132:79–81
    [Google Scholar]
  2. Bergman B. 1984; Photorespiratory ammonium release by the cyanobacterium Anabaena cylindrica in the presence of methionine sulphoximine.. Archives of Microbiology 137:21–25
    [Google Scholar]
  3. Boussiba S., Gibson J. 1985; The role of glutamine synthetase activity in ammonium and methylam- monium transport in Anacystis nidulans R-2.. FEBS Letters 180:13–16
    [Google Scholar]
  4. Boussiba S., Dilling W., Gibson J. 1984a; Methylammonium transport in Anacystis nidulans R-2.. Journal of Bacteriology 160:204–210
    [Google Scholar]
  5. Boussiba S., Resch C.M., Gibson J. 1984b; Ammonia uptake and retention in some cyanobacteria.. Archives of Microbiology 138:287–292
    [Google Scholar]
  6. Bradford M.M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principles of protein-dye binding.. Analytical Biochemistry 72:248–254
    [Google Scholar]
  7. Castorph H., Kleiner D. 1984; Some properties of a Klebsiella pneumoniae ammonium transport negative mutant (Amt).. Archives of Microbiology 139:245–247
    [Google Scholar]
  8. Eady R.R., Kahn D., Buchanan-Wollaston V. 1982; The molecular enzymology of nitrogen fixation.. Israel Journal of Botany 31:45–60
    [Google Scholar]
  9. Franco A.R., Cardenas J., Fernandez E. 1987; A mutant of Chlamydomonas reinhardtii altered in the transport of ammonium and methylammonium.. Molecular and General Genetics 206:414–418
    [Google Scholar]
  10. Hawkesford M.J., Rowell P., Stewart W.D.P. 1983; Energy transduction in cyanobacteria.. In Photosynthetic Prokaryotes: Cell Differentiation and Function pp 199–218 Papageorgiou G. C., Packer L. Edited by New York: Elsevier;
    [Google Scholar]
  11. Helber J., Johnson T.R., Yarbrough L.R., Hirschberg R. 1988; Effect of nitrogenous compounds on nitrogenase gene expression in anaerobic cultures of Anabaena variabilis.. Journal of Bacteriology 170:558–563
    [Google Scholar]
  12. Hien N.T., Kerby N.W., Machray G.C., Rowell P., Stewart W.D.P. 1988; Expression of glutamine synthetase in mutant strains of the cyanobacterium Anabaena variabilis which liberate ammonia.. FEMS Microbiology letters 56:337–342
    [Google Scholar]
  13. Kerby N.W., Rowell P., Stewart W.D.P. 1986a; The uptake and metabolism of methylamine by N2-fixing cyanobacteria.. Archives of Microbiology 143:353–358
    [Google Scholar]
  14. Kerby N.W., Musgrave S. C., Rowell P., Shestakov S. V., Stewart W.D.P. 1986b; Photoproduction of ammonium by immobilised mutant strains of Anabaena variabilis.. Applied Microbiology and Biotechnology 24:42–46
    [Google Scholar]
  15. Kerby N.W., Rowell P., Stewart W.D.P. 1987a; Cyanobacterial ammonium transport, ammonium assimilation and nitrogenase regulation.. New Zealand Journal of Marine and Freshwater Research 21:447–455
    [Google Scholar]
  16. Kerby N.W., Niven G. W., Rowell P., Stewart W.D.P. 1987b; Photoproduction of amino acids by mutant strains of N2-fixing cyanobacteria.. Applied Microbiology and Biotechnology 25:547–552
    [Google Scholar]
  17. Kleiner D. 1985; Energy expenditure for cyclic retention of NH3/NH4 + during N2 fixation by Klebsiella pneumoniae.. FEBS Letters 187:237–239
    [Google Scholar]
  18. Mackinney G. 1941; Absorption of light by chlorophyll solutions.. Journal of Biological Chemistry 140:315–322
    [Google Scholar]
  19. Murry M.A., Benemann J.R. 1979; Nitrogenase regulation in Anabaena cylindrica.. Plant and Cell Physiology 20:1391–1401
    [Google Scholar]
  20. Ohmori M., Ohmori K., Strotmann H. 1977; Inhibition of nitrate uptake by ammonia in a blue- green alga Anabaena cylindrica.. Archives of Microbiology 114:225–229
    [Google Scholar]
  21. Polukhina L.E., Sakhurieva G.N., Shestakov S.V. 1982; Ethylenediamine resistant Anabaena variabilis mutants with derepressed nitrogen fixing system.. Microbiology 51:90–95
    [Google Scholar]
  22. Rai A.N., Bergman B. 1986; Modification of NO3 metabolism in heterocysts of the N2-fixing cyanobacterium Anabaena 7120 (ATCC 27893).. FEMS Microbiology Letters 36:133–137
    [Google Scholar]
  23. Rai A.N., Rowell P., Stewart W.D.P. 1984; Evidence for an ammonium transport system in free living and symbiotic cyanobacteria.. Archives of Microbiology 137:241–246
    [Google Scholar]
  24. Ramos J.L., Madueno F., Guerrero M. 1985; Regulation of nitrogenase levels in Anabaena sp. ATCC 33047 and other filamentous cyanobacteria.. Archives of Microbiology 141:105–111
    [Google Scholar]
  25. Reed R.H., Kerby N.W., Stewart W.D.P. 1987; Particle size analysis of unicells formed by mild sonication of filaments of the blue-green alga Anabaena variabilis.. Phycologia 26:391–393
    [Google Scholar]
  26. Reich S., Almon H., Boger P. 1986; Short-term effect of ammonia on nitrogenase activity of Anabaena variabilis (ATCC 29413).. FEMS Microbiology Letters 34:53–56
    [Google Scholar]
  27. Rippka R., Deruelles J., Waterbury J.B., Herdman M., Stanier R.Y. 1979; Generic assignments, strain histories and properties of pure cultures of cyanobacteria.. Journal of General Microbiology 111:1–61
    [Google Scholar]
  28. Rowell P., Enticott S., Stewart W.D.P. 1977; Glutamine synthetase and nitrogenase activity in the blue-green alga Anabaena cylindrica.. New Phytologist 79:41–54
    [Google Scholar]
  29. Servin-Gonzalez L., Ortiz M., Gonzalez A., Bastarrachea F. 1987; glnA mutations conferring resistance to methylammonium in Escherichia coli K12.. Journal of General Microbiology 133:1631–1639
    [Google Scholar]
  30. Singh D.T., Rai A.N., Singh H.N. 1985; Methylammonium (ammonium) uptake in a glutamine auxotroph of the cyanobacterium Anabaena cycadae.. FEBS Letters 186:51–53
    [Google Scholar]
  31. Singh D.T., Ghosh R., Singh H.N. 1987; Physiological characterisation of the ammonium transport system in the free-living diazotrophic cyanobacterium Anabaena cycadae.. Journal of Plant Physiology 127:231–239
    [Google Scholar]
  32. Singh H.N., Rai U.N., Rao V.V., Bagchi S.N. 1983; Evidence for ammonia as an inhibitor of heterocyst and nitrogenase formation in the cyanobacterium Anabaena cycadae.. Biochemical and Biophysical Research Communications 111:180–187
    [Google Scholar]
  33. Solorzano L. 1969; Determination of ammonia in natural waters by the phenolhypochlorite method.. Limnology and Oceanography 14:799–801
    [Google Scholar]
  34. Stewart W.D.P., Rowell P. 1975; Effects of l- methionine-dl-sulphoximine on the assimilation of newly fixed NH3, acetylene reduction and heterocyst production in Anabaena cylindrica.. Biochemical and Biophysical Research Communications 65:846–857
    [Google Scholar]
  35. Stewart W.D.P., Fitzgerald G.P., Burris R.H. 1967; In situ studies on N2-fixation using the acetylene reduction technique.. Proceedings of the National Academy of Sciences of the United States of America 58:2071–2078
    [Google Scholar]
  36. Stewart W.D.P., Rowell P, Kerby N.W. 1985; Physiological studies on Infixing cyanobacteria.. In Nitrogen Fixation and CO2 Metabolism pp 269–279 Ludden P. W., Burris J. E. Edited by New York: Elsevier;
    [Google Scholar]
  37. Turpin D.H., Edie S.A., Canvin D.T. 1984; In vivo nitrogenase regulation by ammonium and the effect of MSX on ammonium transport in Anabaena flos-aquae.. Plant Physiology 74:701–704
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-6-1441
Loading
/content/journal/micro/10.1099/00221287-135-6-1441
Loading

Data & Media loading...

Most cited Most Cited RSS feed