1887

Abstract

A defined growth medium (designated AP), in which the concentrations of Na and K could be altered independently of one another, was developed for ATCC 25975. The addition of 100 m-Na to AP-medium containing 25 m-K initially reduced the rate of expression of extracellular glucosyltransferase (GTF). However, once had adaptated to grow in the presence of 100 m-Na, the rate of GTF expression was stimulated. In fact once adapted to the presence of Na in the environment the same increase in the rate of enzyme expression was observed in all batch cultures irrespective of the K concentration (2–50 m). At 2 m-K there was no change in the level of saturation of the membrane lipids when the Na concentration was increased from 0 m to 100 m. Na-stimulation of GTF expression was confirmed in non-proliferating cell suspensions at different K concentrations. In non-proliferating cell suspensions, GTF expression outlined a rectangular hyperbola with respect to K concentration when the K concentration was stepped up from 2 m. The increase in GTF synthesis and secretion was transient and was similar to that previously reported by us in Na-rich medium, though it did not reach the same high levels. The reduced transient stimulation of GTF expression correlated both with an enrichment in the saturated fatty acids of the membrane lipids of , and with the fact that the degree of saturation was only slightly reduced when the K concentration was stepped up from 2 m to 50 m. Needless to say, the final octadecenoic to octadecanoic (C/C) fatty acid ratio retained its direct correlation with the transient increase in GTF production following the step up in K concentration, giving rise to an apparent biphasic plot when combined with that previously reported.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-6-1431
1989-06-01
2021-10-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/6/mic-135-6-1431.html?itemId=/content/journal/micro/10.1099/00221287-135-6-1431&mimeType=html&fmt=ahah

References

  1. Bakker E.P., Randall L.L. 1984; The requirement for energy during export of -lactamase in Escherichia coli is fulfilled by the total proton- motive force.. EMBO Journal 3:895–900
    [Google Scholar]
  2. Bakker E.P., Mangerich W.E. 1981; Interconversion of components of the bacterial proton motive force by electrogenic potassium transport.. Journal of Bacteriology 147:820–826
    [Google Scholar]
  3. Chen L., Tai P.C. 1985; ATP is essential for protein translocation into Escherichia coli membrane vesicles.. Proceedings of the National Academy of Sciences of the United States of America 82:4384–4388
    [Google Scholar]
  4. Heefner D.L. 1982; Transport of H+, K+, Na+ and Ca+ in Streptococcus.. Molecular Cell Biochemistry 44:81–106
    [Google Scholar]
  5. Jacques N.A. 1983; Membrane perturbation by cerulenin modulates glucosyltransferase secretion and acetate uptake by Streptococcus salivarius.. Journal of General Microbiology 129:3293–3302
    [Google Scholar]
  6. Kakinuma Y. 1987; Sodium/proton antiporter in Streptococcus faecalis.. Journal of Bacteriology 169:3886–3890
    [Google Scholar]
  7. Kakinuma Y., Harold F.M. 1985; ATP-driven exchange of Na+ and K+ ions by Streptococcus faecalis.. Journal of Biological Chemistry 260:2086–2091
    [Google Scholar]
  8. Kashket E.R., Blanchard A.G., Metzger W.C. 1980; Protonmotive force during growth of Streptococcus cells.. Journal of Bacteriology 143:128–134
    [Google Scholar]
  9. Keevil C.W., West A.A., Bourne N., Marsh P.D. 1983; Synthesis of a fructosyltransferase by Streptococcus sanguis.. FEMS Microbiology Letters 20:155–157
    [Google Scholar]
  10. Keevil C.W., West A.A., Bourne N., Marsh P.D. 1984; Inhibition of the synthesis and secretion of extracellular glucosyl- and fructosyl- transferase in Streptococcus sanguis by sodium ions.. Journal of General Microbiology 130:77–82
    [Google Scholar]
  11. Markevics L.J., Jacques N.A. 1985; Enhanced secretion of glucosyltransferase by changes in potassium ion concentration is accompanied by an altered pattern of membrane fatty acids in Streptococcus salivarius.. Journal of Bacteriology 161:989–994
    [Google Scholar]
  12. Pagès J.M., Lazdunski C. 1982; Membrane potential (Δ) depolarizing agents inhibit maturation of exported proteins in Escherichia coli.. FEBS Letters 149:51–54
    [Google Scholar]
  13. Pirt S.J. 1975 Principles of Microbe and Cell Cultivation, p. 160. Melbourne:: Blackwell.;
    [Google Scholar]
  14. Pitty(Née Markevics) L.J., Jacques N.A. 1987; The influence of incorporation of octade- cenoic acid on the cell-associated fructosyltransfer- ase and the extracellular glucosyltransferase activities of Streptococcus salivarius.. Journal of General Microbiology 133:3565–3573
    [Google Scholar]
  15. Randall L.L., Hardy S.J.S., Thom J.R. 1987; Export of protein: a biochemical view.. Annual Review of Microbiology 41:507–541
    [Google Scholar]
  16. Rosen B.P. 1986; Recent advances in bacterial ion transport.. Annual Review of Microbiology 40:263–286
    [Google Scholar]
  17. Sato Y., Yamamoto Y., Suzuki R. 1987; Effects of potassium ions on lactate production and growth of Streptococcus mutans in relation to the proton motive force.. Bulletin of the Tokyo Dental College 28:99–109
    [Google Scholar]
  18. De Vrije T., De Swart R.L., Dowhan W., Tommassen J., De Kruijff B. 1988; Phospha- tidylglycerol is involved in protein translocation across Escherichia coli inner membranes.. Nature London: 334:173–175
    [Google Scholar]
  19. West A.A., Keevil C.W., Marsh P.D., Ellwood D.C. 1984; The effect of ionophores on growth and glycosyltransferase production of Streptococcus sanguis.. FEMS Microbiology Letters 25:133–137
    [Google Scholar]
  20. West A.A., Whiley R.A., Marsh P.D., Keevil C.W. 1987; The relationship between glycosyltransferase production and membrane fatty acid composition of Streptococcus sanguis WCTC 7865 grown in the presence of protonmotive force inhibitors.. Journal of General Microbiology 133:2601–2606
    [Google Scholar]
  21. Yamane K., Ichihara S., Mizushima S. 1987; In vitro translocation of protein across Escherichia colimembrane vesicles requires both the proton motive force and ATP.. Journal of Biological Chemistry 262:2358–2362
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-6-1431
Loading
/content/journal/micro/10.1099/00221287-135-6-1431
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error