Transport of Benzoic and Propanoic Acids by Free

Abstract

Uptake rates of benzoic and propanoic acid into were proportional to the concentration of undissociated acid and showed no indication of limitation at high concentrations. Benzoic acid permeated 27 times faster than propanoic acid. Other low- fatty acids were taken up at rates approximately related to their lipophilicity. Glucose stimulated uptake rate and inhibitors of glucose transport or metabolism removed the stimulation. It was concluded that the principal mechanism of uptake was diffusion of undissociated acid. The membrane had lower permeabilities than reported for other cell types and lipid bilayers. Growth in the presence of benzoic acid reduced permeability to benzoic acid further. Preservative-resistant yeast species had lower uptake rates of propanoic acid than sensitive ones. Degradation of the cell wall did not change the permeability to propanoic acid.

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1989-05-01
2024-03-28
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References

  1. Cole M.B., Keenan M.H.J. 1987; Effects of weak acids and external pH on the intracellular pH of Zygosaccharomyces bailii, and its implications in weak-acid resistance. Yeast 3:23–32
    [Google Scholar]
  2. Deuticke B. 1977; Properties and structural basis of simple diffusion pathways in the erythrocyte membrane. Review of Physiological and Biochemical Pharmacology 78:1–97
    [Google Scholar]
  3. Dickinson D.P., Isenberg I. 1982; Preparation of spheroplasts of Schizosaccharomyces pombe . Journal of General Microbiology 128:651–654
    [Google Scholar]
  4. Klocke R.A., Flasterstein F. 1982; Kinetics of erythrocyte penetration by aliphatic acids. Journal of Applied Physiology 53:1138–1143
    [Google Scholar]
  5. Macris B.J. 1975; Mechanism of benzoic acid uptake by Saccharomyces cerevisiae . Applied Microbiology 30:503–506
    [Google Scholar]
  6. Macris B.J., Markarkis P. 1974; Transport and toxicity of sulphur dioxide in the yeast Saccharomyces cerevisiae var. ellipsoideus . Journal of the Science of Food and Agriculture 25:21–29
    [Google Scholar]
  7. Stein W.D. 1981; Permeability for lipophilic molecules. In Membrane Transport, pp 1–28 Bonting S. L., De Pont J. J. H. H. M. Edited by Amsterdam: Elsevier;
    [Google Scholar]
  8. Stratford M., Rose A.H. 1986; Transport of sulphur dioxide by Saccharomyces cerevisiae . Journal of General Microbiology 132:1–6
    [Google Scholar]
  9. Thomas D.S., Davenport R.R. 1985; Zygosaccharomyces bailii — a profile of characteristics and spoilage activities. Food Microbiology 2:157–169
    [Google Scholar]
  10. Walter A., Gutknecht J. 1986; Permeability of small nonelectrolytes through lipid bilayer membranes. Journal of Membrane Biology 90:207–217
    [Google Scholar]
  11. Warth A.D. 1977; Mechanism of resistance of Saccharomyces bailii to benzoic, sorbic and other weak acids used as food preservatives. Journal of Applied Bacteriology 43:215–230
    [Google Scholar]
  12. Warth A.D. 1985; Resistance of yeast species to benzoic and sorbic acids and to sulfur dioxide. Journal of Food Protection 48:564–569
    [Google Scholar]
  13. Warth A.D. 1986a; Effect of nutrients and pH on the resistance of Zygosaccharomyces bailii to benzoic acid. International Journal of Food Microbiology 3:263–271
    [Google Scholar]
  14. Warth A.D. 1986b; Preservative resistance of Zygosaccharomyces bailii and other yeasts. CSIRO Food Research Quarterly 46:1–8
    [Google Scholar]
  15. Warth A.D. 1988; Effect of benzoic acid on the growth yield of yeasts differing in their resistance to preservatives. Applied and Environmental Microbiology 54:2091–2095
    [Google Scholar]
  16. Wolosin J.M., Ginsburg H. 1975; The permeation of organic acids through lecithin bilayers resemblance to diffusion in polymers. Biochimica et biophysica acta 389:20–33
    [Google Scholar]
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