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A wall-less ‘slime’ strain of Neurospora crassa was shown to be pleiotropically altered in the production and secretion of several enzymes related to carbon catabolism, including invertase, alkaline protease, aryl-β-glucosidase and cellobiase. Invertase and protease were produced constitutively. The two β-glucosidases required induction by cellobiose but were poorly affected by glucose repression. Exoenzymes were released in larger amounts by the ‘slime’ strain than by the wild-type. Two other carbon-regulated enzymes, isocitrate lyase and phosphoenolpyruvate carboxykinase, were normally regulated in the ‘slime’ strain. A study of recombinants from a cross of a ‘slime’-containing heterokaryon and wild-type suggested that the anomalies observed were inherent in the ‘slime’ phenotype.
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