1887

Abstract

Proteinase activity was detected in culture filtrates of eight wood-decaying basidiomycete fungi and compared in terms of ability to clear skimmed milk agar. All the fungi were proteolytic, but to differing extents. Five were compared using azocasein hydrolysis as a measure of proteolytic activity at the centres and margins of agar-grown colonies and it was found that in the marginal mycelium was the more strongly proteolytic, while in all the other fungi proteolysis by central mycelium was greater. The time course of changes in proteolytic activity in culture filtrates of and grown on the surface of liquid media was compared over 4 weeks, and differences were found which suggested that mycelium inactivates its proteinases after secreting them, but that does not. The results are interpreted in terms of the likely role of proteinases in the nitrogen economy of these fungi when growing on their wood substrates.

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1989-05-01
2021-08-01
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References

  1. Fenn P., Kirk T.K. 1981; Relationship of nitrogen to the onset and suppression of ligninolytic activity and secondary metabolism in Phanerochaete chrysosporium . Archives of Microbiology 130:59–65
    [Google Scholar]
  2. Kalisz H.M., Moore D., Wood D.A. 1986; Protein utilization by Basidiomycete fungi. Transactions of the British Mycological Society 86:519–525
    [Google Scholar]
  3. Kalisz H.M., Wood D.A., Moore D. 1987; Production, regulation and release of extracellular proteinase activity in basidiomycete fungi. Transactions of the British Mycological Society 88:221–227
    [Google Scholar]
  4. Laidlaw R.A., Smith G.A. 1965; The proteins of the timber of Scots pine (Pinus sylvestris). Hotzforschung 19:129–134
    [Google Scholar]
  5. North M.J. 1982; Comparative biochemistry of the proteinases of eukaryotic microorganisms. Microbiological Reviews 46:308–340
    [Google Scholar]
  6. Palmer J.G., Murmanis L., Highley T.L. 1983a; Visualization of hyphal sheath in wood-decay hymenomycetes. I. Brown-rotters. Mycologia 75:995–1004
    [Google Scholar]
  7. Palmer J.G., Murmanis L., Highley T.L. 1983b; Visualization of hyphal sheath in wooddecay hymenomycetes. 11. White-rotters. Mycologi 75:1005–1010
    [Google Scholar]
  8. Prestidge L., Gage V., Spizizen J. 1971; Protease activities during the course of sporulation in Bacillus subtilis . Journal of Bacteriology 107:815–823
    [Google Scholar]
  9. Staszczak M., Nowak G. 1984; Proteinase pattern in Trametes versicolor in response to carbon and nitrogen starvation. Acta biochimica polonica 31:431–438
    [Google Scholar]
  10. Venables C.E., Watkinson S.C. 1989; Medium-induced changes in patterns of free and combined amino acids in mycelium of Serpula lacrimans . Mycological Research (in the Press)
    [Google Scholar]
  11. Wood D.A. 1980; Inactivation of extracellular laccase during fruiting of Agaricus bisporus. Journal of General Microbiology 117:339–345
    [Google Scholar]
  12. Yanagita T., Nomarchi Y. 1967; Kinetic analysis of the region of protease formation in the hypha of Aspergillus niger . Journal of General and Applied Microbiology 13:227–235
    [Google Scholar]
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