Using purine auxotrophic strains of with additional genetic lesions in the pathways of interconversion and salvage of purine compounds, we demonstrated the function of guanosine kinase and inosine kinase. Mutants with increased ability to utilize guanosine were isolated by plating cells on medium with guanosine as the sole purine source. These mutants had altered guanosine kinase activity and the mutations were mapped in the gene encoding guanosine kinase, . Some of the mutants had acquired an additional genetic lesion in the purine biosynthetic pathway, namely a , a or a mutation. A rev-recd map location of the gene is presented and the gene order established as .


Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error