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Volume 135, Issue 5

Anaerobic Growth of on Glycerol by Importing Genes of the Regulon from Free

Abstract

The regulon of specifying fermentative dissimilation of glycerol was mobilized by the broad-host-range plasmid RP4:mini Mu and introduced conjugatively into . The recipient was enabled to grow anaerobically on glycerol without added hydrogen acceptors, although its cell yield was less than that of . The reduced cell yield was probably due to the lack of the coenzyme-B-dependent glycerol dehydratase of the system. This enzyme initiates the first step in an auxiliary pathway for disposal of the extra reducing equivalents from glycerol. The lack of this enzyme would also account for the absence of 1,3-propanediol (a hallmark fermentation product of glycerol) in the spent culture medium. In a control experiment, a large quantity of this compound was detected in a similar culture medium following the growth of . The other three known enzymes of the system, glycerol dehydrogenase, dihydroxyacetone kinase and 1,3-propanediol oxidoreductase, however, were synthesized at levels comparable to those found in . Regulation of the system in appeared to follow the same pattern as in : the three acquired enzymes were induced by glycerol, catabolite repressed by glucose, and glycerol dehydrogenase was post-translationally inactivated during the shift from anaerobic to aerobic growth. The means by which the recipient can achieve redox balance without formation of 1,3-propanediol during anaerobic growth on glycerol remains to be discovered.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
© Society for General Microbiology 1989
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1989-05-01
2024-04-18
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Anaerobic Growth of Escherichia coli on Glycerol by Importing Genes of the dha Regulon from Klebsiella pneumoniae
Microbiology 135, 1255 (1989); https://doi.org/10.1099/00221287-135-5-1255
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